Results 321 to 330 of about 828,765 (377)

Three-dimensional hepatocyte differentiated MSCs vs. 2D cell culture in induction of hepatic regeneration in an experimental model

, 2018
Manal Kamel
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Drug Discovery Approaches Utilizing Three-Dimensional Cell Culture

ASSAY and Drug Development Technologies, 2016
Historically, two-dimensional (2D) cell culture has been the preferred method of producing disease models in vitro. Recently, there has been a move away from 2D culture in favor of generating three-dimensional (3D) multicellular structures, which are thought to be more representative of the in vivo environment.
S. Ryan, A. Baird, Gisela M. F. Vaz, A. Urquhart, hias Senge, D. Richard, K. O'Byrne, A. Davies   +7 more
semanticscholar   +4 more sources

Three-dimensional cell-culture platform based on hydrogel with tunable microenvironmental properties to improve insulin-secreting function of MIN6 cells.

Biomaterials, 2021
Pancreatic β-cells have been reported to be mechanosensitive to cellular microenvironments, and subjecting the cells to more physiologically relevant microenvironments can produce better results than when subjecting them to the conventional two ...
Miao Zhang, Sen Yan, Xueqin Xu, Ting-ting Yu, Zhaobin Guo, Ming Ma, Yi Zhang, Zhuxiao Gu, Yiwei Feng, Chunyue Du, Mengqi Wan, Ke Hu, Xiao Han, N. Gu   +13 more
semanticscholar   +1 more source

Self-Assembly of Dendritic DNA into a Hydrogel: Application in Three-Dimensional Cell Culture.

ACS Applied Materials and Interfaces, 2021
With inherent biocompatibility, biodegradability, and unique programmability, hydrogels with a DNA framework show great potential in three-dimensional (3D) cell culture. Here, a DNA hydrogel was assembled by a dendritic DNA with four branches.
Jingyuan Wu, Bella Rosa Liyarita, Haishuang Zhu, Ming Liu, Xiao Hu, Fangwei Shao   +5 more
semanticscholar   +1 more source

Three-dimensional perfused cell culture

Biotechnology Advances, 2014
Compelling evidence suggests the limitation and shortcomings of the current and well established cell culture method using multi-well plates, flasks and Petri dishes. These are particularly important when cell functions are sensitive to the local microenvironment, cell-cell and cell-extracellular matrix interactions.
Zhaohui, Li, Zhanfeng, Cui
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Three‐dimensional Cell Cultures Mimic Tissuesa

Annals of the New York Academy of Sciences, 1992
Three-dimensional cell culture using gels of type I collagen is a flexible method for studying cell behavior in a tissuelike environment. With only small changes in the basic protocol, we were able to encapsulate neutrophils, hepatocytes, and PC12 cells.
W M, Saltzman, M R, Parkhurst, P, Parsons-Wingerter, W H, Zhu   +3 more
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Three-dimensional microwell arrays for cell culture

Lab Chip, 2011
We propose the concept of three-dimensional (3D) microwell arrays for cell culture applications and highlight the importance of oxygen diffusion through pores in all three dimensions to enhance cell viability.
Christina L, Randall, Yevgeniy V, Kalinin, Mustapha, Jamal, Tanmay, Manohar, David H, Gracias   +4 more
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Three-dimensional cell culturing by magnetic levitation

Nature Protocols, 2013
Recently, biomedical research has moved toward cell culture in three dimensions to better recapitulate native cellular environments. This protocol describes one method for 3D culture, the magnetic levitation method (MLM), in which cells bind with a magnetic nanoparticle assembly overnight to render them magnetic. When resuspended in medium, an external
William L, Haisler, David M, Timm, Jacob A, Gage, Hubert, Tseng, T C, Killian, Glauco R, Souza   +5 more
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Fabrication techniques of biomimetic scaffolds in three‐dimensional cell culture: A review

Journal of Cellular Physiology, 2020
In the last four decades, several researchers worldwide have routinely and meticulously exercised cell culture experiments in two‐dimensional (2D) platforms.
A. K. Badekila, S. Kini, Amit K Jaiswal
semanticscholar   +1 more source

Analysis of Cell Proliferation by Three-Dimensional Culture

2022
Three-dimensional (3D) cell culture technology is a powerful tool in cancer research and drug development. It retains several critical components of the in vivo environment, including cell-cell and cell-extracellular matrix (ECM) interactions in a 3D fashion, gradients of oxygen, nutrients and metabolic waste, and it is thus more physiologically ...
Xiaoyun, Tang, David N, Brindley
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