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Development of Three-Dimensional Cell Culture Scaffolds using Laminin Peptide-Conjugated Agarose Microgels.

Biomacromolecules, 2020
Three-dimensional (3D) cell scaffolds are essential for tissue engineering. So far, various polymer hydrogels have been utilized to design 3D scaffolds as a biomaterial. In this study, we focused on a biocompatible polysaccharide, agarose, as a potential
Yuji Yamada   +4 more
semanticscholar   +1 more source

Studying centrosome function using three-dimensional cell cultures

2015
Three-dimensional (3D) cell cultures have long been recognized as a tool for the study of tissue architecture, polarity, and invasion. However, only recently these systems have been used to study centrosome and cilia functions. Studying these organelles in 3D cultures has elucidated new functions that otherwise would have been overlooked, demonstrating
Teresa, Arnandis, Susana A, Godinho
openaire   +2 more sources

Convenient Three-Dimensional Cell Culture in Supermolecular Hydrogels

ACS Applied Materials & Interfaces, 2014
A convenient three-dimensional cell culture was developed by employing high swelling property of hybrid hydrogels coassembled from C2-phenyl-based supermolecular gelators and sodium hyaluronate. Imaging and spectroscopic analysis by scanning electron microscopy (SEM), atomic force microscopy (AFM), transform infrared (FT-IR) spectra confirm that the ...
Ping, Li   +4 more
openaire   +2 more sources

Electrokinetic Perfusion Through Three-Dimensional Culture Reduces Cell Mortality

Tissue Engineering Part A, 2021
Cell proliferation and survival are dependent on mass transfer. In vivo, fluid flow promotes mass transfer through the vasculature and interstitial space, providing a continuous supply of nutrients and removal of cellular waste products. In the absence of sufficient flow, mass transfer is limited by diffusion and poses significant challenges to cell ...
Anyesha, Sarkar, Mark A, Messerli
openaire   +2 more sources

Alginate Microencapsulation for Three-Dimensional In Vitro Cell Culture

ACS Biomaterials Science & Engineering, 2020
Advances in microscale 3D cell culture systems have helped to elucidate cellular physiology, understand mechanisms of stem cell differentiation, produce pathophysiological models, and reveal important cell-cell and cell-matrix interactions. An important consideration for such studies is the choice of material for encapsulating cells and associated ...
Sung-Min Kang   +3 more
openaire   +2 more sources

In-situ forming thermosensitive hydroxypropyl chitin-based hydrogel crosslinked by Diels-Alder reaction for three dimensional cell culture.

Carbohydrate Polymers, 2019
Injectable thermosensitive hydrogels crosslinked physically have been extensively studied as scaffolds in biomedical field, however, their low gel stability with weak strength limits their potential applications.
Bo Bi   +4 more
semanticscholar   +1 more source

Three-dimensional culture of mesenchymal stem cells

Tissue Engineering and Regenerative Medicine, 2015
Mesenchymal stem cells (MSCs) are partially defined by their ability to differentiate into different tissues in vitro–including the bone, cartilage, and adipose tissue, as well as their ability to secrete bioactive factors in response to local microenvironmental cues.
Jong Kyu Hong   +3 more
openaire   +1 more source

Injectable hydrogels based on glycyrrhizin, alginate, and calcium for three-dimensional cell culture in liver tissue engineering.

Journal of Biomedical Materials Research. Part A, 2018
Injectable hydrogels have been paid more attentions on cell therapy and tissue regeneration resulting from the applications in minimally invasive surgical procedures with ease of handling and complete filling of defect area.
Xiao-fang Tong   +5 more
semanticscholar   +1 more source

Microfluidic engineered high cell density three-dimensional neural cultures

Journal of Neural Engineering, 2007
Three-dimensional (3D) neural cultures with cells distributed throughout a thick, bioactive protein scaffold may better represent neurobiological phenomena than planar correlates lacking matrix support. Neural cells in vivo interact within a complex, multicellular environment with tightly coupled 3D cell-cell/cell-matrix interactions; however, thick 3D
D Kacy, Cullen   +3 more
openaire   +2 more sources

A thixotropic nanocomposite gel for three-dimensional cell culture

Nature Nanotechnology, 2008
Thixotropic materials, which become less viscous under stress and return to their original state when stress is removed, have been used to deliver gel-cell constructs and therapeutic agents. Here we show that a polymer-silica nanocomposite thixotropic gel can be used as a three-dimensional cell culture material.
Y Shona, Pek   +4 more
openaire   +2 more sources

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