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Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids

2004
The chloroplasts contain an extensive system of internal membranes or thylakoids in which all the light-harvesting and energy-transducing processes of the photosynthesis are located. Thylakoids are differentiated into stacked membrane regions (or grana thylakoids) and nonstacked membranes (or stroma thylakoids), each with a specialized structure and ...
Juan, Cuello, María José, Quiles
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Differential scanning calorimetric investigation of pea chloroplast thylakoids and thylakoid fractions

Archives of Biochemistry and Biophysics, 1992
High sensitivity differential scanning calorimetry (DSC) was employed to study the thermal denaturation of components of pea chloroplast thylakoid membranes. In contrast to previous reports utilizing spinach thylakoids, several transitions are reversible, and deconvolution of the calorimetric curves indicates nine transitions in both first and second ...
W G, Nolan, H P, Hopkins, S A, Kalini
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Thylakoid membranes: The translational site of chloroplast DNA-regulated thylakoid polypeptides

Archives of Biochemistry and Biophysics, 1984
Stromal ribosomes and those bound to thylakoid membranes were prepared from intact spinach chloroplasts which were purified on Percoll gradients. The products of read-out translation of these ribosomes supplemented with an Escherichia coli extract were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
E, Minami, A, Watanabe
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Redox regulation in the thylakoid lumen

Journal of Plant Physiology, 2016
Higher plants need to balance the efficiency of light energy absorption and dissipative photo-protection when exposed to fluctuations in light quantity and quality. This aim is partially realized through redox regulation within the chloroplast, which occurs in all chloroplast compartments except the envelope intermembrane space.
Zhen-Hui, Kang, Gui-Xue, Wang
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Thylakoid membrane proteomics

Photosynthesis Research, 2003
Proteomics seeks to monitor the global complement of proteins within a cell or organism and accompanying plasticity with respect to development and environment. The proteome is dynamic, the product of current and past gene expression, countless protein-protein interactions and selective proteolytic systems.
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Getting into thylakoids

Trends in Cell Biology, 1999
A dynamic synergy currently holds sway in the study of protein export across the bacterial inner membrane and chloroplast thylakoids. Initially, ideas flowed from the eubacterial to the thylakoid field, with chloroplast workers confirming the presence of Sec, signal-recognition particle (SRP) and spontaneous pathways in their system.
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Macroorganisation and flexibility of thylakoid membranes

Biochemical Journal, 2019
Abstract The light reactions of photosynthesis are hosted and regulated by the chloroplast thylakoid membrane (TM) — the central structural component of the photosynthetic apparatus of plants and algae. The two-dimensional and three-dimensional arrangement of the lipid–protein assemblies, aka macroorganisation, and its dynamic responses ...
Petar H. Lambrev, Parveen Akhtar
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PROTEIN TARGETING TO THE THYLAKOID MEMBRANE

Annual Review of Plant Physiology and Plant Molecular Biology, 1998
▪ Abstract  The assembly of the photosynthetic apparatus at the thylakoid begins with the targeting of proteins from their site of synthesis in the cytoplasm or stroma to the thylakoid membrane. Plastid-encoded proteins are targeted directly to the thylakoid during or after synthesis on plastid ribosomes.
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Properties of thylakoids and thylakoid particles derived from structurally different chloroplasts

Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1981
Abstract Structurally and functionally different tobacco chloroplasts were subjected to digitonin treatment and subsequent fractional centrifugation. The light-harvesting chlorophyll a chlorophyll b- protein complex was found to be enriched in the most dense fraction regardless of the presence of grana in the original preparation.
Peter H. Homann, Yuan-Yuan Liu
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The Electrochemical Properties of the Thylakoid Membrane

1999
This chapter is focused on the electrochemical properties of the thylakoid membrane and illustrates their dependence on energization. Topics include primary reactions, charge separation, charge recombination, secondary electron flow, protonmotive force, charge dissipation and membrane conductance.
Snel, J.F.H., Vredenberg, W.J.
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