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Polylysine effect on thylakoid membranes

Biophysical Chemistry, 2020
Thylakoid membranes of photosynthetic plant chloroplasts are involved in a wide range of energy producing pathways. Their stacking can be employed in order to provide increased surface area for biocatalytic purposes. Here we probe the aggregate formation of higher plants' thylakoids using low-molecular poly-l-lysine as an electrostatic polymer linker ...
Virjinia, Doltchinkova   +1 more
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Differential scanning calorimetric investigation of pea chloroplast thylakoids and thylakoid fractions

Archives of Biochemistry and Biophysics, 1992
High sensitivity differential scanning calorimetry (DSC) was employed to study the thermal denaturation of components of pea chloroplast thylakoid membranes. In contrast to previous reports utilizing spinach thylakoids, several transitions are reversible, and deconvolution of the calorimetric curves indicates nine transitions in both first and second ...
W G, Nolan, H P, Hopkins, S A, Kalini
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Patterns of thylakoid system formation

Journal of Ultrastructure Research, 1979
Plastid differentiation has been studied in leaves of both tobacco and wheat. We could point out a correlation between the differentiation speed of the plastids and that of the cell containing them. The ontogenetic patterns of both tobacco and wheat plastids appear to be different from the standard ones. The significance of these particular ontogeneses
CASADORO, GIORGIO, RASCIO, NICOLETTA
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Thylakoid membranes: The translational site of chloroplast DNA-regulated thylakoid polypeptides

Archives of Biochemistry and Biophysics, 1984
Stromal ribosomes and those bound to thylakoid membranes were prepared from intact spinach chloroplasts which were purified on Percoll gradients. The products of read-out translation of these ribosomes supplemented with an Escherichia coli extract were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
E, Minami, A, Watanabe
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Thylakoid membrane architecture

Australian Journal of Plant Physiology, 1999
Confocal scanning laser microscopic observations were made on live chloroplasts in intact cells and on mechanically isolated, intact chloroplasts. Chlorophyll fluorescence was imaged to observe thylakoid membrane architecture. C3 plant species studied included Spinacia oleracea L., Spathiphyllum sp. Schott, cv. ‘Mauna Loa’, and Pisum sativum L.
Mehta, M., Sarafis, V., Critchley, C.
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Chloroplast thylakoid membrane‐stabilised emulsions

Journal of the Science of Food and Agriculture, 2010
AbstractBACKGROUND: Thylakoid‐stabilised emulsions have been reported to possess satiety‐promoting effects and inhibit pancreatic lipase–colipase activity in vitro, which prompted the investigation of their interfacial properties.RESULTS: Thylakoid membranes isolated from spinach were used as an emulsifier/stabiliser in oil (triglyceride)‐in‐water ...
Marilyn, Rayner   +5 more
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Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids

2004
The chloroplasts contain an extensive system of internal membranes or thylakoids in which all the light-harvesting and energy-transducing processes of the photosynthesis are located. Thylakoids are differentiated into stacked membrane regions (or grana thylakoids) and nonstacked membranes (or stroma thylakoids), each with a specialized structure and ...
Juan, Cuello, María José, Quiles
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Sub-thylakoid fractions containing ribosomes

Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1980
A sub-membrane fraction which contains a large portion of any thylakoid-bound ribosomes can be obtained when thylakoids are treated with the detergents Nonidet P-40, or Triton X-100. These 'pseudopolysome' fractions contain 50% of thylakoid-bound ribosomes, but less than 0.5% of thylakoid chlorophyll. Triton and Nonidet psuedopolysomes contain about 10%
M M, Margulies, J S, Weistrop
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