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Fractionation of Thylakoid Membranes Into Grana and Stroma Thylakoids

2004
The chloroplasts contain an extensive system of internal membranes or thylakoids in which all the light-harvesting and energy-transducing processes of the photosynthesis are located. Thylakoids are differentiated into stacked membrane regions (or grana thylakoids) and nonstacked membranes (or stroma thylakoids), each with a specialized structure and ...
Juan Cuello, María José Quiles
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Differential scanning calorimetric investigation of pea chloroplast thylakoids and thylakoid fractions

Archives of Biochemistry and Biophysics, 1992
High sensitivity differential scanning calorimetry (DSC) was employed to study the thermal denaturation of components of pea chloroplast thylakoid membranes. In contrast to previous reports utilizing spinach thylakoids, several transitions are reversible, and deconvolution of the calorimetric curves indicates nine transitions in both first and second ...
William G. Nolan   +2 more
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Patterns of thylakoid system formation

Journal of Ultrastructure Research, 1979
Plastid differentiation has been studied in leaves of both tobacco and wheat. We could point out a correlation between the differentiation speed of the plastids and that of the cell containing them. The ontogenetic patterns of both tobacco and wheat plastids appear to be different from the standard ones. The significance of these particular ontogeneses
CASADORO, GIORGIO, RASCIO, NICOLETTA
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Polylysine effect on thylakoid membranes

Biophysical Chemistry, 2020
Thylakoid membranes of photosynthetic plant chloroplasts are involved in a wide range of energy producing pathways. Their stacking can be employed in order to provide increased surface area for biocatalytic purposes. Here we probe the aggregate formation of higher plants' thylakoids using low-molecular poly-l-lysine as an electrostatic polymer linker ...
Victoria Vitkova, Virjinia Doltchinkova
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Thylakoid membranes: The translational site of chloroplast DNA-regulated thylakoid polypeptides

Archives of Biochemistry and Biophysics, 1984
Stromal ribosomes and those bound to thylakoid membranes were prepared from intact spinach chloroplasts which were purified on Percoll gradients. The products of read-out translation of these ribosomes supplemented with an Escherichia coli extract were analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
Akira Watanabe, Eiichi Minami
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Getting into thylakoids

Trends in Cell Biology, 1999
A dynamic synergy currently holds sway in the study of protein export across the bacterial inner membrane and chloroplast thylakoids. Initially, ideas flowed from the eubacterial to the thylakoid field, with chloroplast workers confirming the presence of Sec, signal-recognition particle (SRP) and spontaneous pathways in their system.
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Thylakoid membrane proteomics

Photosynthesis Research, 2003
Proteomics seeks to monitor the global complement of proteins within a cell or organism and accompanying plasticity with respect to development and environment. The proteome is dynamic, the product of current and past gene expression, countless protein-protein interactions and selective proteolytic systems.
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Redox regulation in the thylakoid lumen

Journal of Plant Physiology, 2016
Higher plants need to balance the efficiency of light energy absorption and dissipative photo-protection when exposed to fluctuations in light quantity and quality. This aim is partially realized through redox regulation within the chloroplast, which occurs in all chloroplast compartments except the envelope intermembrane space.
Guixue Wang, Zhen-Hui Kang
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Photoelectrochemistry of thylakoid membranes

Bioelectrochemistry and Bioenergetics, 1997
Abstract A one-compartment three-electrode photoelectrochemical cell, first designed by Allen and Crane and recently modified by Carpentier, was used to study the chronoamperometric behavior of a suspension of thylakoids under saturating light conditions. The experimental results were modeled successfully by considering the processes occurring in the
AGOSTIANO, Angela, M. CASELLI
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Thylakoid membrane architecture

Functional Plant Biology, 1999
Confocal scanning laser microscopic observations were made on live chloroplasts in intact cells and on mechanically isolated, intact chloroplasts. Chlorophyll fluorescence was imaged to observe thylakoid membrane architecture. C3 plant species studied included Spinacia oleracea L., Spathiphyllum sp. Schott, cv. ‘Mauna Loa’, and Pisum sativum L.
Mehta, M., Sarafis, V., Critchley, C.
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