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Photosynthesis Research, 2003
Proteomics seeks to monitor the global complement of proteins within a cell or organism and accompanying plasticity with respect to development and environment. The proteome is dynamic, the product of current and past gene expression, countless protein-protein interactions and selective proteolytic systems.
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Proteomics seeks to monitor the global complement of proteins within a cell or organism and accompanying plasticity with respect to development and environment. The proteome is dynamic, the product of current and past gene expression, countless protein-protein interactions and selective proteolytic systems.
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Physical Chemistry Chemical Physics, 2014
Thylakoid membranes from spinach were separated into grana and stroma thylakoid fractions which were characterized by several methods (pigment content, protein gel electrophoresis, photosystem activities, and electron microscopy analysis) to confirm that the intact thylakoids were differentiated into the two domains. The results of photoelectrochemical
Michelle, Rasmussen, Shelley D, Minteer
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Thylakoid membranes from spinach were separated into grana and stroma thylakoid fractions which were characterized by several methods (pigment content, protein gel electrophoresis, photosystem activities, and electron microscopy analysis) to confirm that the intact thylakoids were differentiated into the two domains. The results of photoelectrochemical
Michelle, Rasmussen, Shelley D, Minteer
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Properties of thylakoids and thylakoid particles derived from structurally different chloroplasts
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1981Abstract Structurally and functionally different tobacco chloroplasts were subjected to digitonin treatment and subsequent fractional centrifugation. The light-harvesting chlorophyll a chlorophyll b- protein complex was found to be enriched in the most dense fraction regardless of the presence of grana in the original preparation.
Peter H. Homann, Yuan-Yuan Liu
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Proteomic Analysis of Thylakoid Membranes
2010Chlamydomonas is a model organism to study photosynthesis. Thylakoid membranes comprise several proteins belonging to photosystems I and II. In this chapter, we show the accurate proteomic measurements in thylakoid membranes. The chlorophyll-containing membrane protein complexes were precipitated using chloroform/methanol solution. These complexes were
Venkateswarlu, Yadavalli +2 more
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Trends in Cell Biology, 1999
A dynamic synergy currently holds sway in the study of protein export across the bacterial inner membrane and chloroplast thylakoids. Initially, ideas flowed from the eubacterial to the thylakoid field, with chloroplast workers confirming the presence of Sec, signal-recognition particle (SRP) and spontaneous pathways in their system.
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A dynamic synergy currently holds sway in the study of protein export across the bacterial inner membrane and chloroplast thylakoids. Initially, ideas flowed from the eubacterial to the thylakoid field, with chloroplast workers confirming the presence of Sec, signal-recognition particle (SRP) and spontaneous pathways in their system.
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Thylakoid Phosphoproteins: Identification of Phosphorylation Sites
2010Redox-dependent thylakoid protein phosphorylation regulates both the short- and long-term acclimation of the photosynthetic apparatus to changes in environmental conditions. The major thylakoid phosphoproteins belong to photosystem II (D1, D2, CP43, PsbH) and its light-harvesting antenna (Lhcb1, Lhcb2, CP29), but a number of minor phosphoproteins have ...
Rokka, A, Aro, EM, Vener, A
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Photoelectrochemistry of thylakoid membranes
Bioelectrochemistry and Bioenergetics, 1997Abstract A one-compartment three-electrode photoelectrochemical cell, first designed by Allen and Crane and recently modified by Carpentier, was used to study the chronoamperometric behavior of a suspension of thylakoids under saturating light conditions. The experimental results were modeled successfully by considering the processes occurring in the
AGOSTIANO, Angela, M. CASELLI
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Carbonic Anhydrase Activities in Pea Thylakoids
Photosynthesis Research, 2004Pea thylakoids with high carbonic anhydrase (CA) activity (average rates of 5000 micromol H(+) (mg Chl)(-1) h(-1) at pH 7.0) were prepared. Western blot analysis using antibodies raised against the soluble stromal beta-CA from spinach clearly showed that this activity is not a result of contamination of the thylakoids with the stromal CA but is derived
O V, Moskvin +7 more
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Subfractionation of Cyanobacterial Thylakoids
1989Cyanobacteria are free-living procaryotes capable of oxygenic photosynthesis, containing both photosystem I and photosystem II [1] including the oxygen evolving complex. Cyanobacteria as a model system for studying the oxygenic photosynthesis has a great advantage compared to plant chloroplasts; being a procaryote organism it permits the use of modern ...
Fredrik Nilsson +3 more
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Evolution of Thylakoid Structure
2005Photosynthesis, an ancient process, originated among the earliest forms of life. Its broad distribution through at least half of the eubacterial phyla is an indication of this antiquity and, as stated by Woese (1987), the complexity of this process deems it unlikely that such a process arose on multiple occasions.
Gregory R. Wolfe, J. Kenneth Hoober
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