CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point +7 more
wiley +1 more source
Dynamic connectivities of plant metacommunities at a millennial time-scale: the Beringia testbed
Herzschuh U +23 more
europepmc +1 more source
Long-Time Scale Simulations Reveal Key Dynamics That Drive the Onset of the N State in the Proteorhodopsin Photocycle. [PDF]
Billings KR, Faramarzi S, Mertz B.
europepmc +1 more source
Sustainable bioplastics manufacturing from renewable sources
Bioplastics are manufactured by using polymers from different bio‐based sources. These novel materials not only offer biodegradability but also possess various functional properties that make them suitable for diverse applications. Recent developments in the preparation of bioplastics are reported, highlighting the distinct properties of each type of ...
C. Valeria L. Giosafatto +6 more
wiley +1 more source
Partial asynchrony of coniferous forest carbon sources and sinks at the intra-annual time scale. [PDF]
Silvestro R +72 more
europepmc +1 more source
Aged human bmMSCs are seeded in the scaffold. Osteoblastic induction can slightly increase cell's bone‐forming activity to produce bone‐like tissues, shown as the sporadic xylenol orange‐stained spots (the lower left image). Notably, pioglitazone plus EGCG co‐treatment dramatically increases cell's bone‐forming activity and bone‐like tissue production (
Ching‐Yun Chen +6 more
wiley +1 more source
Atomistic Mechanism of Lipid Membrane Binding for Blood Coagulation Factor VIII with Molecular Dynamics Simulations on a Microsecond Time Scale. [PDF]
Avery NG +3 more
europepmc +1 more source
Enzymatic degradation of biopolymers in amorphous and molten states: mechanisms and applications
This review explains how polymer morphology and thermal state shape enzymatic degradation pathways, comparing amorphous and molten biopolymer structures. By integrating structure–reactivity principles with insights from thermodynamics and enzyme engineering, it highlights mechanisms that enable efficient polymer breakdown.
Anđela Pustak, Aleksandra Maršavelski
wiley +1 more source
Joint modeling in presence of informative censoring on the retrospective time scale with application to palliative care research. [PDF]
Wu Q +4 more
europepmc +1 more source

