Results 41 to 50 of about 424,131 (275)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

A case of orbital extra-adrenal paraganglioma in a quarter horse

Brazilian Journal of Veterinary Pathology, 2019
Paragangliomas are tumors originating from paraganglia cells which represent a considerable constituent of the dispersed neuroendocrine system. Rarely these tumors are found within the orbital region. This report documents a case of primary extra-adrenal
Letizia Tomassini, Ilaria Pettinari, Elvio Lepri, Rodolfo Gialletti, Enrico Bellezza, Leonardo Leonardi   +5 more
doaj   +1 more source

BCG vaccination potentiates oxidative phosphorylation in neonatal myeloid‐derived suppressor cells

FEBS Open Bio, EarlyView.
BCG vaccination enhances oxidative phosphorylation in neonatal MDSCs, impairing their immunosuppressive function. It upregulates electron transport chain genes and mitochondrial activity, increasing ATP and oxygen consumption. Pharmacological OXPHOS inhibition partially restores suppressive capacity, confirming causality.
Yingying Chen, Hui Li
wiley   +1 more source

IGF2 knockout reduces but does not abolish osteosarcoma growth in vitro and in vivo

FEBS Open Bio, EarlyView.
To test whether endogenous IGF2 promotes osteosarcoma growth, IGF2 was knocked out in Saos2 cells via CRISPR‐Cas9. KO cells showed reduced proliferation in vitro, and knockout xenografts in mice reached only ~25% of wild‐type tumor volume. Insulin‐like growth factor 2 (IGF2) is implicated in osteosarcoma, but direct functional evidence of its role is ...
Shun Yao, Marco Archetti
wiley   +1 more source

Influences on the Decision to Euthanize a Compromised Pig

Animals
The decision to euthanize a compromised pig can be challenging for pig farmers and veterinarians. To understand more about the challenges in Germany, a cross-sectional online survey was conducted.
Julia Stoffregen, Tristan Winkelmann, Bettina Schneider, Michel Fehrmann, Kathrin Gerdes, Moana Miller, Jennifer Reinmold, Isabel Hennig-Pauka, Nicole Kemper, Christin Kleinsorgen, Karl-Heinz Tölle, Lothar Kreienbrock, Michael Wendt, Elisabeth grosse Beilage   +13 more
doaj   +1 more source

A Holistic Platform Approach for Developing Robust Pulmonary mRNA Delivery: Integrating Novel Phenolic Acid‐Derived Ionizable Lipids and Device Engineering

Advanced Functional Materials, EarlyView.
An integrated pulmonary mRNA delivery platform combining novel biodegradable syringic acid‐derived ionizable lipids, design‐of‐experiments formulation optimization, and vibrating‐mesh nebulizer engineering enabled stable aerosolization and efficient lung delivery.
Neha Kaushal, Younan Ma, Asad Khanmohammed, Dipen Parande, Hanming Zhang, Saket Dolare, Juliet Chen, Apiwat Wangweerawong, Saswata Karmakar, Hongfeng Deng, Frank Koppenhagen, Shraddha Sharma, Eric Reyes, Sherry He, Lianne Boeglin, Caroline Woo, Azadeh Paksa, Ayed Allawzi, Anusha Dias, Gregory Troiano, Shrirang Karve, Frank DeRosa   +21 more
wiley   +1 more source

Electrocution as an alternative euthanasia method to blunt force trauma to the head followed by exsanguination for non-viable piglets

Acta Veterinaria Scandinavica, 2020
Background On farms, the currently approved and most widely practised method of euthanising non-viable piglets is blunt force trauma to the head followed by exsanguination.
Johannes Husheer, Matthias Luepke, Peter Dziallas, Karl-Heinz Waldmann, Alexandra von Altrock   +4 more
doaj   +1 more source

A survey of dairy cattle veterinarians’ perspectives on timely management and euthanasia for common adult cow conditions

, 2019
Timely euthanasia of compromised animals on dairy farms can reduce the poor welfare outcomes when recovery is prolonged or impossible. However, little to now research exists regarding euthanasia decision-making.
Proudfoot, Kathryn L., Dietsch, Alia M., Shearer, Jan K., Pairis-Garcia, Monique D., Cramer, M. Caitlin   +4 more
core   +1 more source

Protein‐Polymer Dynamic Hydrogels Utilizing Native Ligand Binding for Injectable Therapeutic Delivery and 3D Printing

Advanced Healthcare Materials, EarlyView.
Dynamic nanocolloidal hydrogels were synthesized via the co‐assembly of ligand‐modified spheroidal hyperbranched poly(glycerol) (HPG) and soluble albumin nanoparticles (SANs). Co‐assembly occurred through self‐association of the HPG, as well as its interaction with SANs via native ligand binding.
S. Cem Millik, Yonghui Wang, Kefan Song, Jessica M. Snyder, LeAnn Le, Lauren G. Brown, Shanny Hsuan Kuo, Ashleigh B. Theberge, Suzie H. Pun, Alshakim Nelson   +9 more
wiley   +1 more source

Clinical, laboratory and ultrasonographic findings in 87 cows with type-4 abomasal ulcer

BMC Veterinary Research, 2019
Background This study evaluated the clinical, laboratory, ultrasonographic and pathological findings in 87 cows aged 2 to 10 years (4.5 ± 1.5 years) with type-4 abomasal ulcer.
Ueli Braun, Christina Reif, Karl Nuss, Monika Hilbe, Christian Gerspach   +4 more
doaj   +1 more source