Culture of periprosthetic tissue in blood culture bottles for diagnosing periprosthetic joint infection [PDF]
Background: The purpose of this meta-analysis was to evaluate the diagnostic accuracy of periprosthetic tissue culture in blood culture bottles (BCB) for periprosthetic joint infection (PJI).
Li, Cheng+2 more
core +1 more source
Plants regenerated from tissue culture contain stable epigenome changes in rice. [PDF]
Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps of DNA methylation in several regenerated rice ...
Bellizzi, Maria+8 more
core +2 more sources
High-throughput design of cultured tissue moulds using a biophysical model [PDF]
The technique presented here identifies tethered mould designs, optimised for growing cultured tissue with very highly-aligned cells. It is based on a microscopic biophysical model for polarised cellular hydrogels. There is an unmet need for tools to assist mould and scaffold designs for the growth of cultured tissues with bespoke cell organisations ...
arxiv
Maintenance of head and neck tumor on-chip: gateway to personalized treatment? [PDF]
Aim: Head and neck squamous cell carcinomas (HNSCC) are solid tumors with low overall survival (40–60%). In a move toward personalized medicine, maintenance of tumor biopsies in microfluidic tissue culture devices is being developed. Methodology/ results:
Bower, Ruth+7 more
core +1 more source
A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson+31 more
core +2 more sources
Epigenetics in plant tissue culture [PDF]
Plants produced vegetatively in tissue culture may differ from the plants from which they have been derived. Two major classes of off-types occur: genetic ones and epigenetic ones. This review is about epigenetic aberrations.
Klerk, G.J.M., de, Smulders, M.J.M.
core +6 more sources
Occurrence of spontaneous polyploids in Citrus
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman+3 more
doaj +1 more source
Neuronal micro-culture engineering by microchannel devices of cellular scale dimensions [PDF]
Purpose: The purpose of the current study was to investigate the effect of microchannel geometry on neuronal cultures and to maintain these cultures for long period of time (over several weeks) inside the closed microchannels of cellular scale dimensions.
arxiv +1 more source
Probing the pH microenvironment of mesenchymal stromal cell cultures on additive-manufactured scaffolds [PDF]
Despite numerous advances in the field of tissue engineering and regenerative medicine, monitoring the formation of tissue regeneration and its metabolic variations during culture is still a challenge and mostly limited to bulk volumetric assays. Here, a simple method of adding capsules based optical sensors in cell seeded 3D scaffolds is presented and
arxiv +1 more source
Hollow Fiber Bioreactors for In Vivo-like Mammalian Tissue Culture [PDF]
Tissue culture has been used for over 100 years to study cells and responses ex vivo. The convention of this technique is the growth of anchorage dependent cells on the 2-dimensional surface of tissue culture plastic.
Ellis, MJ+7 more
core +1 more source