Results 41 to 50 of about 2,255,345 (306)

Doubled haploid ramets via embryogenesis of haploid tissue cultures [PDF]

, 2010
Tissue culture in the oil palm business is generally concerned with the multiplication (clonal production) of dura, pisifera and tenera palms. These are all normal diploids (2n=2x=36).
Caligari, P. D. S., Dunwell, Jim M., Forster, B. P., Iswandar, H. E., Nelson, S. P. C.   +4 more
core  

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

In vitro and in vivo validation of human and goat chondrocyte labeling by green fluorescent protein lentivirus transduction [PDF]

, 2010
We investigated whether human articular chondrocytes can be labeled efficiently and for long-term with a green fluorescent protein (GFP) lentivirus and whether the viral transduction would influence cell proliferation and tissue-forming capacity.
Acharya, Chitrangada, Barbero, Andrea, Candrian, Christian, Gianni-Barrera, Roberto, Jaquiery, Claude, Juelke, Henriette, Mainil-Varlet, Pierre, Martin, Ivan, Miot, Sylvie, Pelttari, Karoliina   +9 more
core   +1 more source

The newfound relationship between extrachromosomal DNAs and excised signal circles

FEBS Letters, EarlyView.
Extrachromosomal DNAs (ecDNAs) contribute to the progression of many human cancers. In addition, circular DNA by‐products of V(D)J recombination, excised signal circles (ESCs), have roles in cancer progression but have largely been overlooked. In this Review, we explore the roles of ecDNAs and ESCs in cancer development, and highlight why these ...
Dylan Casey, Zeqian Gao, Joan Boyes
wiley   +1 more source

Single ChAdOx1 nCoV‐19 dose elicits stronger immune response in previously infected individuals than in SARS‐CoV2 naive persons

Immunity, Inflammation and Disease
Background Current vaccines against COVID‐19 effectively reduce morbidity and mortality and are vitally important for controlling the pandemic. Between December 2020 and February 2021, adenoviral vector vaccines such as ChAdOx1 (AstraZeneca‐Oxford) were ...
Fawzi Ebrahim, Asma Alboueishi, Inas M. Alhudiri, Salah Al Tabal, Yosra Lamami, Samira Al Dwigen, Sondos Ashleb, Noha Ejenfawi, Mohamed B. Milad, Hayat Rhoumah, Salah Edin El Meshri, Adam Elzagheid   +11 more
doaj   +1 more source

Structural instability impairs function of the UDP‐xylose synthase 1 Ile181Asn variant associated with short‐stature genetic syndrome in humans

FEBS Letters, EarlyView.
The Ile181Asn variant of human UDP‐xylose synthase (hUXS1), associated with a short‐stature genetic syndrome, has previously been reported as inactive. Our findings demonstrate that Ile181Asn‐hUXS1 retains catalytic activity similar to the wild‐type but exhibits reduced stability, a looser oligomeric state, and an increased tendency to precipitate ...
Tuo Li, Pedro A. Sánchez‐Murcia, Bernd Nidetzky   +2 more
wiley   +1 more source

Harnessing in vitro technologies for conservation of Paeonia emodi, a threatened medicinal and ornamental species

Discover Plants
Paeonia emodi Wall. ex Royle, a valuable medicinal and ornamental species native to the north-western Himalayas, is threatened by overharvesting and habitat loss [1, 2].
Munisa Manzoor, Nidha Zahoor Shahri, Irshad Ahmad Bhat, Zahoor A Kaloo   +3 more
doaj   +1 more source

Induction of Polyploidy in Citrus Rootstocks through In Vitro Colchicine Treatment of Seed-Derived Explants

Agronomy, 2023
Polyploidy, frequently observed in citrus species, aids in achieving better adaptation to environmental stresses. In this context, the current work aims to develop stable tetraploids in citrus rootstock cultivars, viz., Rough lemon, Rangpur lime and ...
Vijayakumari Narukulla, Yogesh Lahane, Krutika Fiske, Shashi Pandey, Vasileios Ziogas   +4 more
doaj   +1 more source

Three-dimensional tissue scaffolds from interbonded poly(e-caprolactone) fibrous matrices with controlled porosity

, 2011
In this article, we report on the preparation and cell culture performance of a novel fibrous matrix that has an interbonded fiber architecture, excellent pore interconnectivity, and controlled pore size and porosity.
Kirkland, Mark, Lin, Tong, Sutti, Alessandra, Tang, Yanwei, Wang, Hongxia, Wang, Xungai, Wong, Cynthia   +6 more
core   +1 more source

Organ‐specific redox imbalances in spinal muscular atrophy mice are partially rescued by SMN antisense oligonucleotides

FEBS Letters, EarlyView.
We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley   +1 more source