Co-culture of Adult Mesenchymal Stem Cells and Nucleus Pulposus Cells in Bilaminar Pellets for Intervertebral Disc Regeneration [PDF]
Background: Our goal is to optimize stem cell-based tissue engineering strategies in the context of the intervertebral disc environment. We explored the benefits of co-culturing nucleus pulposus cells (NPC) and adult mesenchymal stem cells (MSC) using a ...
Allon, AA, Lotz, JC, Schneider, RA
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Bacteria are the causative agents of numerous diseases. Ever increasing number of bacterial infections has generated the need to find new antibiotic materials and new ways to combat bacterial infections.
Gandhimathi Chinnasamy +3 more
doaj +1 more source
Plants regenerated from tissue culture contain stable epigenome changes in rice. [PDF]
Most transgenic crops are produced through tissue culture. The impact of utilizing such methods on the plant epigenome is poorly understood. Here we generated whole-genome, single-nucleotide resolution maps of DNA methylation in several regenerated rice ...
Bellizzi, Maria +8 more
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A miniaturized bioreactor system for the evaluation of cell interaction with designed substrates in perfusion culture [PDF]
In tissue engineering, the chemical and topographical cues within three-dimensional (3D) scaffolds are normally tested using static cell cultures but applied directly to tissue cultures in perfusion bioreactors.
Anderson +31 more
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Endogenous musculoskeletal tissue engineering - a focused perspective [PDF]
Two major difficulties facing widespread clinical implementation of existing Tissue Engineering (TE) strategies for the treatment of musculoskeletal disorders are (1) the cost, space and time required for ex vivo culture of a patient’s autologous cells ...
Adam, Clayton
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Occurrence of spontaneous polyploids in Citrus
Seedlings recovered from underdeveloped seeds of different commercial cultivars of Citrus were studied for their ploidy level. Cytological examination revealed that maximum triploid plants were found in lime Kaghzi (15.5%) followed by grapefruit Foster ...
M. Usman +3 more
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Studies of intercellular invasion in vitro using rabbit peritoneal neutrophil granulocytes (PMNS). I. Role of contact inhibition of locomotion. [PDF]
Intercellular invasion is the active migration of cells on one type into the interiors of tissues composed of cells of dissimilar cell types. Contact paralysis of locomotion is the cessation of forward extension of the pseudopods of a cell as a result of
Armstrong, Peter, Lackie, J
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Optimization of total RNA isolation method from the aromatic medicinal plant Artemisia annua L.
This paper is the first report on the development of a protocol that allows rapid and simplified extraction of total RNA from Artemisia annua L., an aromatic medicinal plant.
Suganthi Appalasamy +5 more
doaj +1 more source
Treatment of Ligament Constructs with Exercise-conditioned Serum: A Translational Tissue Engineering Model. [PDF]
In vitro experiments are essential to understand biological mechanisms; however, the gap between monolayer tissue culture and human physiology is large, and translation of findings is often poor.
Baar, Keith +2 more
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Computational fluid dynamic analysis of bioprinted self-supporting perfused tissue models [PDF]
Natural tissues are incorporated with vasculature, which is further integrated with a cardiovascular system responsible for driving perfusion of nutrient‐rich oxygenated blood through the vasculature to support cell metabolism within most cell‐dense ...
Bonewald, Lynda F. +8 more
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