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Advancing the 3Rs in bone tissue engineering: emerging <i>in vitro</i>, <i>in silico</i>, and refined <i>in vivo</i> strategies. [PDF]
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Basic Techniques in Mammalian Cell Tissue Culture
Current Protocols in Cell Biology, 2015AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
Katy Phelan
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Mammalian Cell Tissue Culture Techniques
Current Protocols in Molecular Biology, 2016AbstractCultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with
Katy, Phelan, Kristin M, May
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A technique for the freeze-fracture of tissue culture
Journal of Cell Science, 1978ABSTRACT Cultures of rat cerebellum grown on glass coverslips coated first with collodion and then collagen are fixed in glutaraldehyde to which ruthenium red has been added. After fixation, the collodion comes off the glass coverslips and the 3 layers: collodion-collagen-culture are handled as one piece. Following immersion in glycerol,
L, Prescott, M W, Brightman
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Mutagenesis Techniques in Plant Tissue Cultures
2003Mutant plant cell cultures can be useful in the study of the physiology and genetics of plants, as well for the improvement of crops. For recent reviews of mutant cell selection from tissue cultures, the reader is referred to Duncan and Widholm (1), Bright et al. (2), Flick (3), and Chapters 39 - 42 , in this vol. Often these mutants have been obtained
J M, Nelshoppen, J M, Widholm
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Electrophysiological Techniques in Dissociated Tissue Culture
1989The application of electrophysiological techniques to tissue culture is still evolving. We have attempted in this chapter to give a practical summary of intracellular recording techniques used in our laboratory, as well as give some examples of new experimental strategies and electrophysiological methods that should provide further information on a ...
G L, Westbrook, P G, Nelson
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Overview of Cell and Tissue Culture Techniques
Current Protocols in Pharmacology, 1999AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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1982
Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
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Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
openaire +1 more source