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Mutagenesis Techniques in Plant Tissue Cultures
2003Mutant plant cell cultures can be useful in the study of the physiology and genetics of plants, as well for the improvement of crops. For recent reviews of mutant cell selection from tissue cultures, the reader is referred to Duncan and Widholm (1), Bright et al. (2), Flick (3), and Chapters 39 - 42 , in this vol. Often these mutants have been obtained
J M, Nelshoppen, J M, Widholm
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Electrophysiological Techniques in Dissociated Tissue Culture
1989The application of electrophysiological techniques to tissue culture is still evolving. We have attempted in this chapter to give a practical summary of intracellular recording techniques used in our laboratory, as well as give some examples of new experimental strategies and electrophysiological methods that should provide further information on a ...
G L, Westbrook, P G, Nelson
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Techniques for Mammalian Cell Tissue Culture
Current Protocols in Human Genetics, 1994AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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1982
Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
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Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
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Overview of Cell and Tissue Culture Techniques
Current Protocols in Pharmacology, 1999AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
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Basic Techniques in Mammalian Cell Tissue Culture
Current Protocols in Cell Biology, 2007AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
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Plant tissue culture techniques—Tools in plant micropropagation
Current Opinion in Biotechnology, 2011European Biotechnology Congress -- SEP 28-OCT 01, 2011 -- Istanbul ...
Onay, Ahmet +4 more
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Plant Tissue Culture Techniques for Breeding
2018Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been applied successfully in many species and have been used to create plants that are disease and insect resistant and tolerant to water stress and demonstrate enhanced growth and improved yields and much more.
Mark P. Bridgen +2 more
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Techniques for Selecting Mutants from Plant Tissue Cultures
2003The theory and goals of mutant selection from plant tissue cultures have been reviewed extensively over the past few years (I-5), and will only be dealt with in a cursory manner in this chapter. For more detail, the reader is encouraged to consult these review articles. The ideal plant tissue culture system is useful for mutation selection because it:
D R, Duncan, J M, Widholm
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Clonal propagation of Callistemon by tissue culture techniques
Plant Cell Reports, 1982Callus development in Callistemon viminalis was readily achieved when axillary buds derived from nodal tissue were placed in a medium containing macro- and micro-nutrients, sucrose (0.06 M), inositol (300 μM), nicotinic acid (20 μM), pyridoxine hydrochloride (3 μM), thiamine hydrochloride (2 μM), riboflavin (10 μM), cytokinins (5 μM) and auxins (0.1 μM)
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