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Mammalian Cell Tissue Culture Techniques

Current Protocols in Molecular Biology, 2016
AbstractCultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with
Katy Phelan, Kristin M. May
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Basic Techniques in Mammalian Cell Tissue Culture

Current Protocols in Toxicology, 2015
AbstractCultured mammalian cells are used extensively in cell biology studies. It requires a number of special skills in order to be able to preserve the structure, function, behavior, and biology of the cells in culture. This unit describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media
Katy Phelan, Kristin M. May
semanticscholar   +7 more sources

Tissue Culture Techniques [PDF]

open access: possible, 1982
Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
J. Bonga
openaire   +2 more sources

Plant Tissue Culture Techniques for Breeding

2018
Several tissue culture techniques can be used to generate genetic variation in plants. These in vitro procedures have been applied successfully in many species and have been used to create plants that are disease and insect resistant and tolerant to water stress and demonstrate enhanced growth and improved yields and much more.
Tom Eeckhaut   +2 more
openaire   +3 more sources

Overview of Cell and Tissue Culture Techniques

Current Protocols in Pharmacology, 1999
AbstractCulture preparations vary greatly in complexity, ranging from single isolated cells to three‐dimensional histotypic cell structures. Besides cultures obtained directly from animal tissues (primary cultures), permanent cultures of continuously dividing (immortalized) cells have been established.
P. Honegger
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Application of Tissue Culture Techniques to Horticultural Crops

1989
The first and, to date, the most extensive practical application of tissue culture techniques to horticultural crops involves the multiplication of ornarmental plant species. The early work by Morel (4) with orchids, the development of the Murashige and Skoog medium (5), and the efforts of Murashige (6, 7) to enhance the practical side of tissue ...
K. C. Torres
openaire   +3 more sources

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