Uniportal endoscopic decompression and debridement for infectious diseases of spine with neurological deficits: a retrospective study in China. [PDF]
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Enhanced application potential of alveolar organoids through epithelial and niche cell interactions. [PDF]
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Mammalian Cell Tissue Culture Techniques
Current Protocols in Molecular Biology, 2016AbstractCultured mammalian cells are used extensively in molecular biology studies. A number of special skills are required in order to preserve the structure, function, behavior, and biology of cells in culture. This appendix describes the basic skills required to maintain and preserve cell cultures: maintaining aseptic technique, preparing media with
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Technique for visualizing papovaviruses in tumors and in tissue cultures [PDF]
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A technique for the freeze-fracture of tissue culture
Journal of Cell Science, 1978ABSTRACT Cultures of rat cerebellum grown on glass coverslips coated first with collodion and then collagen are fixed in glutaraldehyde to which ruthenium red has been added. After fixation, the collodion comes off the glass coverslips and the 3 layers: collodion-collagen-culture are handled as one piece. Following immersion in glycerol,
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Tissue Culture Techniques [PDF]
Tissue culture is a technique in which small tissue pieces or organs are removed from a donor plant and cultured aseptically on a nutrient medium. By manipulating the chemical composition of the nutrient medium and other environmental parameters, the growth and development of the tissues in culture can be directed into different channels.
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Techniques for Mammalian Cell Tissue Culture
Current Protocols in Human Genetics, 1994AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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