Results 311 to 320 of about 1,547,111 (374)
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TISSUE CULTURE TECHNIQUES IN THE STUDY OF ANIMAL VIRUSES
The American Journal of the Medical Sciences, 1950John F. Enders, Frederick C. Robbins
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TISSUE CULTURE TECHNIQUES FOR SCREENING OF PROSTHETIC MATERIALS*
Annals of the New York Academy of Sciences, 1968Ruth I. Johnsson, Andrew F. Hegyeli
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A technique for the freeze-fracture of tissue culture
Journal of Cell Science, 1978ABSTRACT Cultures of rat cerebellum grown on glass coverslips coated first with collodion and then collagen are fixed in glutaraldehyde to which ruthenium red has been added. After fixation, the collodion comes off the glass coverslips and the 3 layers: collodion-collagen-culture are handled as one piece. Following immersion in glycerol,
Milton W. Brightman, Lise Prescott
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Techniques for Mammalian Cell Tissue Culture
Current Protocols in Human Genetics, 1994AbstractThis appendix opens with detailed discussions on the latest principles of sterile technique and preparation of culture media. Step‐by‐step protocols describe trypsinizing and subculturing monolayer cultures, passaging suspension cultures, freezing and thawing cells, counting cells using a hemacytometer, and preparing cells for transport.
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Tissue Culture Techniques: Introduction
2021Cell culture techniques were developed as a way to study animal cells in vitro. The behavior of single cells, organ culture and monolayer cells can be studied without the in vivo influences arising from physiological and environmental variations among experimental animals.
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Mutagenesis Techniques in Plant Tissue Cultures
2003Mutant plant cell cultures can be useful in the study of the physiology and genetics of plants, as well for the improvement of crops. For recent reviews of mutant cell selection from tissue cultures, the reader is referred to Duncan and Widholm (1), Bright et al. (2), Flick (3), and Chapters 39 - 42 , in this vol. Often these mutants have been obtained
Joan M. Nelshoppen, Jack M. Widholm
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Clonal propagation of Callistemon by tissue culture techniques [PDF]
Plant Cell Reports, 1982 Callus development in Callistemon viminalis was readily achieved when axillary buds derived from nodal tissue were placed in a medium containing macro- and micro-nutrients, sucrose (0.06 M), inositol (300 μM), nicotinic acid (20 μM), pyridoxine hydrochloride (3 μM), thiamine hydrochloride (2 μM), riboflavin (10 μM), cytokinins (5 μM) and auxins (0.1 μM)
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Electrophysiological Techniques in Dissociated Tissue Culture
1989The application of electrophysiological techniques to tissue culture is still evolving. We have attempted in this chapter to give a practical summary of intracellular recording techniques used in our laboratory, as well as give some examples of new experimental strategies and electrophysiological methods that should provide further information on a ...
Phillip G. Nelson, Gary L. Westbrook
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A novel technique to overcome browning in tissue culture
Plant Cell Reports, 1991Experiments conducted using Dioscorea alata L. revealed that an exudate from the cut end of the explants was responsible for browning of the culture medium. Browning did not affect growth of roots and shoots when explants were cultured in a large volume of medium, but in a small volume it was lethal.
K. P. S. Chandel, S. R. Bhat
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Utilization of tissue culture techniques in a seed potato tuber production scheme
, 2006Otroshy, Mahmoud, 2006. Utilization of tissue culture techniques in a seed potato tuber production scheme. Doctoral thesis, Wageningen University, Wageningen, The Netherlands, 264 pp., English and Dutch summaries.
M. Otroshy
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