This protocol is to fix freshly collected plant tissue for serial sectioning. Although this protocol is similar to ones used forin situ mRNA hybridization, it should not be used for that purpose as it is not nearly careful/gently enough. This protocol has been tested with tomato, tobacco, and date fruits with good results.
Alex Rajewski
openaire +2 more sources
Revisiting PFA-mediated tissue fixation chemistry: <i>FixEL</i> enables trapping of small molecules in the brain to visualize their distribution changes. [PDF]
Nonaka H +14 more
europepmc +2 more sources
Outcomes of an All-Soft Tissue Fixation Technique for Reconstruction of the Medial Patellofemoral Complex Using Double-Bundle Quadriceps Tendon Autograft for Recurrent Patellar Dislocation in Skeletally Immature Patients. [PDF]
Zein AMN +4 more
europepmc +3 more sources
Treatment of breast ptosis with submuscular implants and points of attachment of the breast to the pectoralis muscle: technical considerations and outcome assessment [PDF]
Introduction: Mastopexy associated with implant placement is challenging for plastic surgeons. The objective is to describe the placement of a submuscular implant with anatomical detachment in combination with stable fixation of the breast tissue to the ...
Antonio Chiquetti +1 more
doaj +1 more source
Volatile organic chemicals (VOCs) are routinely used for processing biological tissue samples in clinical laboratories. Recognizing their serious health and environmental impacts, a few non-volatile green solvents (choline based ionic liquids, ILs) were ...
P.R. Deepa +3 more
doaj +1 more source
Classification of Molecular Biomarkers
A “biomarker” is any measurable characteristic that indicates the presence or absence of disease or the biological response to a stimulus, typically an exposure or intervention.
Ankeet Shah, Dominic C. Grimberg, Brant A. Inman
doaj +1 more source
A Novel Fixation Method for Variable-Sized Endoscopic Submucosal Dissection Specimens: An In Vitro Animal Experiment. [PDF]
Endoscopic submucosal dissection is considered a curative and minimally invasive treatment for early gastric cancer; however, precise pathologic assessment of resected specimens is required to develop further treatment plans.
Seung Han Kim +11 more
doaj +1 more source
The choice of tissue fixation is critical for preserving the morphology and biochemical information of tissues. Fragile oral tissues with lower tensile strength are challenging to process for histological applications as they are prone to processing ...
Pooja Lahiri +8 more
doaj +1 more source
Extracellular space preservation aids the connectomic analysis of neural circuits
Dense connectomic mapping of neuronal circuits is limited by the time and effort required to analyze 3D electron microscopy (EM) datasets. Algorithms designed to automate image segmentation suffer from substantial error rates and require significant ...
Marta Pallotto +4 more
doaj +1 more source

