Results 11 to 20 of about 4,427,559 (374)

Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining

International Journal of Cell Biology, 2016
Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions.
Kazuo Katoh
doaj   +2 more sources

Optimal Fixation Conditions and DNA Extraction Methods for MLPA Analysis on FFPE Tissue-Derived DNA [PDF]

American Journal of Clinical Pathology, 2016
Objectives: Molecular genetic analysis of formalin-fixed, paraffin-embedded (FFPE) tissues is of great importance both for research and diagnostics. Multiplex ligation-dependent probe amplification (MLPA) is a widely used technique for gene copy number ...
Lilit Atanesyan, Maryvonne Steenkamer, A Horstman, Cathy B. Moelans, Jan P. Schouten, Suvi Savola   +5 more
openalex   +2 more sources

Histological assessment of PAXgene tissue fixation and stabilization reagents.

PLoS ONE, 2011
Within SPIDIA, an EC FP7 project aimed to improve pre analytic procedures, the PAXgene Tissue System (PAXgene), was designed to improve tissue quality for parallel molecular and morphological analysis.
Marcel Kap, Frank Smedts, Wolter Oosterhuis, Rosa Winther, Nanna Christensen, Bilge Reischauer, Christian Viertler, Daniel Groelz, Karl-Friedrich Becker, Kurt Zatloukal, Rupert Langer, Julia Slotta-Huspenina, Koppany Bodo, Bas de Jong, Uwe Oelmuller, Peter Riegman   +15 more
doaj   +2 more sources

Plant Tissue Fixation v1

protocols.io, 2018
This protocol is to fix freshly collected plant tissue for serial sectioning. Although this protocol is similar to ones used forin situ mRNA hybridization, it should not be used for that purpose as it is not nearly careful/gently enough. This protocol has been tested with tomato, tobacco, and date fruits with good results.
A. Rajewski
openaire   +3 more sources

Formalin tissue fixation biases myelin-sensitive MRI. [PDF]

Magn Reson Med, 2019
Purpose Chemical fixatives, such as formalin, form cross-links between proteins and affect the relaxation times and diffusion properties of tissue. These fixation-induced changes likely also affect myelin density measurements produced by quantitative ...
Seifert AC, Umphlett M, Hefti M, Fowkes M, Xu J.   +4 more
europepmc   +2 more sources

Non-reversible tissue fixation retains extracellular vesicles for in situ imaging. [PDF]

Nat Methods, 2019
Gupta MP, Tandalam S, Ostrager S, Lever AS, Fung AR, Hurley DD, Alegre GB, Espinal JE, Remmel HL, Mukherjee S, Levine BM, Robins RP, Molina H, Dill BD, Kenific CM, Tuschl T, Lyden D, D'Amico DJ, Pena JTG.   +18 more
europepmc   +2 more sources

Immunohistochemistry of colorectal cancer biomarker phosphorylation requires controlled tissue fixation. [PDF]

PLoS One, 2014
Phosphorylated signaling molecules are biomarkers of cancer pathophysiology and resistance to therapy, but because phosphoprotein analytes are often labile, poorly controlled clinical laboratory practices could prevent translation of research findings in
Theiss AP, Chafin D, Bauer DR, Grogan TM, Baird GS.   +4 more
europepmc   +2 more sources

Formalin-free fixation and xylene-free tissue processing preserves cell-hydrogel interactions for histological evaluation of 3D calcium alginate tissue engineered constructs

Frontiers in Biomaterials Science, 2023
Histological evaluation of tissue-engineered products, including hydrogels for cellular encapsulation, is a critical and invaluable tool for assessing the product across multiple stages of its lifecycle from manufacture to implantation.
Iran Augusto Da Silva, Iran Augusto Da Silva, Iran Augusto Da Silva, Iran Augusto Da Silva, Nika Gvazava, Nika Gvazava, Nika Gvazava, Nika Gvazava, Indra Putra Wendi, Indra Putra Wendi, Indra Putra Wendi, Indra Putra Wendi, Rodrigo Guinea, Rodrigo Guinea, Rodrigo Guinea, Rodrigo Guinea, Francisco García Giménez, Francisco García Giménez, Francisco García Giménez, Francisco García Giménez, Francisco García Giménez, John Stegmayr, John Stegmayr, John Stegmayr, John Stegmayr, Oxana Klementieva, Oxana Klementieva, Darcy E. Wagner, Darcy E. Wagner, Darcy E. Wagner, Darcy E. Wagner   +30 more
doaj   +1 more source

Fast-tracking Histopathology: Is Microwave the Way Forward? [PDF]

Journal of Clinical and Diagnostic Research, 2019
Introduction: The conventional method of tissue processing involves a long turnaround time. An early diagnosis of tissue biopsies would enable quick decision making and prompt institution of therapy.
Anamika Sinha, Sukumar Santosh Kumar
doaj   +1 more source

Measurement of breast-tissue x-ray attenuation by spectral imaging: fresh and fixed normal and malignant tissue [PDF]

Physics in Medicine & Biology, 63(23) (2018), 2021
Knowledge of x-ray attenuation is essential for developing and evaluating x-ray imaging technologies. In mammography, measurement of breast density, dose estimation, and differentiation between cysts and solid tumours are example applications requiring accurate data on tissue attenuation.
arxiv   +1 more source