Results 211 to 220 of about 393,517 (265)
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Fixation of Tissue for Histochemistry
1991The nature of the morphological information sought from any tissue dictates the fixation technique which is used. The first decision to be made is whether fixation will inhibit any subsequent technique to be used, for example, the use of some monoclonal antibodies in which case fixation will be postponed.
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1960
Electron micrographs of mature plant cells fixed in neutral osmium tetroxide solutions frequently show disrupted cell membranes and an apparently dispersed cytoplasm. In the case of young non-vacuolated plant cells their fixation has been improved by the addition of various agents such as Locke’s medium and dextrin (1) or sucrose (2) to a buffered ...
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Electron micrographs of mature plant cells fixed in neutral osmium tetroxide solutions frequently show disrupted cell membranes and an apparently dispersed cytoplasm. In the case of young non-vacuolated plant cells their fixation has been improved by the addition of various agents such as Locke’s medium and dextrin (1) or sucrose (2) to a buffered ...
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The use of osmium in the fixation and staining of tissues
Proceedings of the Royal Society of London. Series B - Biological Sciences, 1957Abstract The use of gallic acid derivatives in the visualization of osmium in tissue sections has been re-investigated. By the use of alkyl esters of gallic acid greatly improved results can be obtained. Fixation with buffered osmium textroxide followed by ethyl gallate affords a simple and reliable method for staining fat droplets ...
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Fixation of Drosophila Tissues for Immunofluorescence
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe fruit fly Drosophila melanogaster has long been used to study the genetic factors involved in development, and the ability to localize molecules within the organism that allow genetic manipulation can be quite useful. This article discusses some of the issues relating to fixation of various Drosophila tissues for analysis by ...
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Fixation and tissue preservation for antibody studies
The Histochemical Journal, 1972The methods of fixation and preparation of lymphoid tissues for the immunoenzyme technique are reviewed. For this technique an enzyme is used first as an antigen and then as a marker to demonstrate its specific antibody. A variety of commonly employed fixatives satisfactorily conserve tissues for the light microscopic detection of antibody but, for ...
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Cell and tissue fixation, 1972?1982
The Histochemical Journal, 1985There have been changes in the practice of fixation over the past 10 years. There seem to be at least two different pressures working. On the one hand, there is increasing diversity in cell biological techniques which in turn demands more variable fixation procedures. Some of these have been outlined.
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Effect of tricomplex fixation on lung tissue
Journal of Ultrastructure Research, 1972The effect of tricomplex fixation on lungs fixed through vascular perfusion or through instillation of fixatives into the airways has been studied. To test the specificity of electron dense precipitates for phospholipids, some preparations were treated with tricomplex fixatives after lipid extraction with chloroform—methanol in a Soxhlet apparatus. The
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[Fixation of tissue specimens].
Morphologiai es igazsagugyi orvosi szemle, 1981Authors studied the efficacy of various fixatives used in pathohistology. For general purposes and for impregnation techniques sodium-acetate-formalin, for the demonstration of cytoplasmic granula of endocrine cells and for staining of connective tissue elements Ort's solution and formalin with chrome-alum should be used.
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