Results 211 to 220 of about 389,621 (264)
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Fixation of Soft Tissue to Bone
Journal of the American Academy of Orthopaedic Surgeons, 2016A myriad of orthopaedic injuries require surgical fixation of torn connective tissue to an osseous insertion site with either direct repair or reconstruction with a soft-tissue graft. Numerous factors influence the strength of a soft-tissue-to-bone fixation construct, including tissue quality, implant strength, contact area and pressure, and tensioning.
Brian J, Cole +4 more
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Fixation and Staining of Testicular Tissue
Journal of Histotechnology, 1994AbstractThe aim of this study was to determine the optimal conditions for the processing and staining of specimens obtained by fine needle tissue aspiration biopsy (FNTAB) of the testis. We assessed the 5 most commonly cited fixative solutions, the 6 most popular staining schedules, the effects of 6 hour and overnight dehydration and infiltration ...
Con Mallidis +4 more
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Tissue Harvesting and Fixation
2003Fixation is the chemical or physical process that allows tissue sections to be viewed in a close approximation to the living tissue.1 Histological fixation practices have been derived from many other fields, such as the leather tanning industry. Fixation is the single most important factor in achieving a well-prepared section for microscopic analysis ...
Linda L. Jenkins, Karen J. L. Burg
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Fixation, Embedding, and Sectioning of Plant Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
Detlef, Weigel, Jane, Glazebrook
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Microwave fixation of large tissue
Proceedings, annual meeting, Electron Microscopy Society of America, 1990Chemical fixation of large biological tissues in electron microscopy has a disadvantage that fixative doesn’t penetrate well into the deeper part of the tissue. My experience suggests that the poor penetration by chemical fixation can be improved by microwave fixation.
Toshio Sakai, Koji Uchizono
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CO2 Fixation in the Nervous Tissue
1971Publisher Summary This chapter explains CO 2 fixation in the nervous tissue. The primary CO 2 fixation reaction occurs at the oxaloacetate level. Both pyruvate carboxylase and phosphoenolpyruvate carboxykinase reactions lead to the formation of oxaloacetate, while the reaction of malic enzyme synthesizes malate.
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The use of osmium in the fixation and staining of tissues
Proceedings of the Royal Society of London. Series B - Biological Sciences, 1957Abstract The use of gallic acid derivatives in the visualization of osmium in tissue sections has been re-investigated. By the use of alkyl esters of gallic acid greatly improved results can be obtained. Fixation with buffered osmium textroxide followed by ethyl gallate affords a simple and reliable method for staining fat droplets ...
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Fixation of Drosophila Tissues for Immunofluorescence
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe fruit fly Drosophila melanogaster has long been used to study the genetic factors involved in development, and the ability to localize molecules within the organism that allow genetic manipulation can be quite useful. This article discusses some of the issues relating to fixation of various Drosophila tissues for analysis by ...
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Fixation of Tissue for Histochemistry
1991The nature of the morphological information sought from any tissue dictates the fixation technique which is used. The first decision to be made is whether fixation will inhibit any subsequent technique to be used, for example, the use of some monoclonal antibodies in which case fixation will be postponed.
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Fixation and tissue preservation for antibody studies
The Histochemical Journal, 1972The methods of fixation and preparation of lymphoid tissues for the immunoenzyme technique are reviewed. For this technique an enzyme is used first as an antigen and then as a marker to demonstrate its specific antibody. A variety of commonly employed fixatives satisfactorily conserve tissues for the light microscopic detection of antibody but, for ...
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