Results 251 to 260 of about 621,620 (285)

Tissue Harvesting and Fixation

2003
Fixation is the chemical or physical process that allows tissue sections to be viewed in a close approximation to the living tissue.1 Histological fixation practices have been derived from many other fields, such as the leather tanning industry. Fixation is the single most important factor in achieving a well-prepared section for microscopic analysis ...
Linda L. Jenkins, Karen J. L. Burg
openaire   +1 more source

Microwave fixation of large tissue

Proceedings, annual meeting, Electron Microscopy Society of America, 1990
Chemical fixation of large biological tissues in electron microscopy has a disadvantage that fixative doesn’t penetrate well into the deeper part of the tissue. My experience suggests that the poor penetration by chemical fixation can be improved by microwave fixation.
Toshio Sakai, Koji Uchizono
openaire   +1 more source

Cell and tissue fixation, 1972?1982

The Histochemical Journal, 1985
There have been changes in the practice of fixation over the past 10 years. There seem to be at least two different pressures working. On the one hand, there is increasing diversity in cell biological techniques which in turn demands more variable fixation procedures. Some of these have been outlined.
openaire   +2 more sources

Fixation of plant tissue

1960
Electron micrographs of mature plant cells fixed in neutral osmium tetroxide solutions frequently show disrupted cell membranes and an apparently dispersed cytoplasm. In the case of young non-vacuolated plant cells their fixation has been improved by the addition of various agents such as Locke’s medium and dextrin (1) or sucrose (2) to a buffered ...
openaire   +1 more source

Fixation and Permeabilization of Cells and Tissues

Cold Spring Harbor Protocols, 2008
INTRODUCTIONFluorescence microscopy is used to visualize specific cellular components in as native a state and organization as possible. This article describes some of the main issues that must be considered when cells and tissues are fixed and permeabilized. To preserve cellular structure, the specimen is fixed chemically to retain the cells or tissue
Fischer, Andrew H., Jacobson, Kenneth A., Rose, Jack, Zeller, Rolf   +3 more
openaire   +2 more sources

Fixation, Embedding, and Sectioning of Plant Tissues

Cold Spring Harbor Protocols, 2008
INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
Detlef, Weigel, Jane, Glazebrook
openaire   +2 more sources

CO2 Fixation in the Nervous Tissue

1971
Publisher Summary This chapter explains CO 2 fixation in the nervous tissue. The primary CO 2 fixation reaction occurs at the oxaloacetate level. Both pyruvate carboxylase and phosphoenolpyruvate carboxykinase reactions lead to the formation of oxaloacetate, while the reaction of malic enzyme synthesizes malate.
openaire   +2 more sources

Tissue Processing: IV. Applied Fixation

1991
Although reactions with proteins are of overriding importance in tissue fixation (cf. Sect. 12.2), an appreciation of the reactions involving other macromolecules and lipids is also important. A selection of these are surveyed in Table 13.1.
Lyon, Hans, van Deurs, Bo, Høyer, Poul Erik, Prentø, Poul, Møller, Morten   +4 more
openaire   +1 more source

STABILIZING BRAIN TISSUE DURING FIXATION

Archives of Neurology And Psychiatry, 1923
In the fixation of tissue it is the aim to hold the structures as nearly as possible in the form they had during life, or at the moment when the fixing reagent acted. Exceptionally, an artefact may have a differentiating value; with this we are not concerned here.
openaire   +1 more source

Fixation and tissue preservation for antibody studies

The Histochemical Journal, 1972
The methods of fixation and preparation of lymphoid tissues for the immunoenzyme technique are reviewed. For this technique an enzyme is used first as an antigen and then as a marker to demonstrate its specific antibody. A variety of commonly employed fixatives satisfactorily conserve tissues for the light microscopic detection of antibody but, for ...
openaire   +2 more sources