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Transcardiac Perfusion of the Mouse for Brain Tissue Dissection and Fixation.
Bio-protocol, 2021Transcardiac perfusion with saline followed by 4% paraformaldehyde (PFA) is widely used to clear blood and preserve brain for immunostaining or in situ hybridization. PFA breaks into formaldehyde in solution, which cross-link protein and DNA molecules to
Jinyun Wu +5 more
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American Journal of Sports Medicine, 2019
Background: It is controversial whether soft tissue fixation only and bone-plug techniques for medial meniscal allograft transplantation provide equivalent fixation and restoration of load distribution.
L. Ambra +5 more
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Background: It is controversial whether soft tissue fixation only and bone-plug techniques for medial meniscal allograft transplantation provide equivalent fixation and restoration of load distribution.
L. Ambra +5 more
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Lysozyme antigenicity and tissue fixation
Histochemistry, 1978The preservation of lysozyme (LZM) antigenicity was studied in paraffin embedded tissue blocks. The reactivity for LZM varied with the type of tissue studied, the fixative used, the osmolarity and pH of the fixative, fixation time and temperature, and the method of dehydration. In both rat and human tissues aqueous fixatives were superior to nonaqueous
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Fixation of Mouse Embryos and Tissues
Cold Spring Harbor Protocols, 2007INTRODUCTIONThis protocol provides methods for fixation of mouse embryos and tissues with paraformaldehyde, Bouin’s fixative, or methanol/DMSO solution.
Andras, Nagy +3 more
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Tissue Harvesting and Fixation
2003Fixation is the chemical or physical process that allows tissue sections to be viewed in a close approximation to the living tissue.1 Histological fixation practices have been derived from many other fields, such as the leather tanning industry. Fixation is the single most important factor in achieving a well-prepared section for microscopic analysis ...
Linda L. Jenkins, Karen J. L. Burg
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Microwave fixation of large tissue
Proceedings, annual meeting, Electron Microscopy Society of America, 1990Chemical fixation of large biological tissues in electron microscopy has a disadvantage that fixative doesn’t penetrate well into the deeper part of the tissue. My experience suggests that the poor penetration by chemical fixation can be improved by microwave fixation.
Toshio Sakai, Koji Uchizono
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1960
Electron micrographs of mature plant cells fixed in neutral osmium tetroxide solutions frequently show disrupted cell membranes and an apparently dispersed cytoplasm. In the case of young non-vacuolated plant cells their fixation has been improved by the addition of various agents such as Locke’s medium and dextrin (1) or sucrose (2) to a buffered ...
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Electron micrographs of mature plant cells fixed in neutral osmium tetroxide solutions frequently show disrupted cell membranes and an apparently dispersed cytoplasm. In the case of young non-vacuolated plant cells their fixation has been improved by the addition of various agents such as Locke’s medium and dextrin (1) or sucrose (2) to a buffered ...
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Fixation and Permeabilization of Cells and Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONFluorescence microscopy is used to visualize specific cellular components in as native a state and organization as possible. This article describes some of the main issues that must be considered when cells and tissues are fixed and permeabilized. To preserve cellular structure, the specimen is fixed chemically to retain the cells or tissue
Fischer, Andrew H. +3 more
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Fixation, Embedding, and Sectioning of Plant Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
Detlef, Weigel, Jane, Glazebrook
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CO2 Fixation in the Nervous Tissue
1971Publisher Summary This chapter explains CO 2 fixation in the nervous tissue. The primary CO 2 fixation reaction occurs at the oxaloacetate level. Both pyruvate carboxylase and phosphoenolpyruvate carboxykinase reactions lead to the formation of oxaloacetate, while the reaction of malic enzyme synthesizes malate.
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