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Effect of formalin tissue fixation and processing on immunohistochemistry.
American Journal of Surgical Pathology, 2000Although immunohistochemistry is routinely performed by many pathology laboratories, its standardization still lags behind. A major cause of variation in the reproducibility of immunohistochemical staining is induced by tissue fixation and, to a lesser ...
Martin Werner +3 more
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The Fixation of Soluble Antigen by the Tissues
Journal of Infectious Diseases, 1912On the injection of an antigen into a susceptible animal a reaction takes place resulting in the formation of antibodies specific for the antigen injected; therefore a study of the seat of fixation of antigen is of importance in the study of antibody formation.
R. T. Pettit, A. J. Carlson
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Fixation of Drosophila Tissues for Immunofluorescence
Cold Spring Harbor Protocols, 2011INTRODUCTIONThe fruit fly Drosophila melanogaster has long been used to study the genetic factors involved in development, and the ability to localize molecules within the organism that allow genetic manipulation can be quite useful. This article discusses some of the issues relating to fixation of various Drosophila tissues for analysis by ...
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Fixation, Embedding, and Sectioning of Plant Tissues
Cold Spring Harbor Protocols, 2008INTRODUCTIONThis protocol describes the preparation of plant tissues for sectioning and subsequent use in immunohistochemistry or in situ hybridization. Tissues of interest are fixed and embedded in Paraplast before sectioning.
Jane Glazebrook, Detlef Weigel
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CO2 Fixation in the Nervous Tissue
1971Publisher Summary This chapter explains CO 2 fixation in the nervous tissue. The primary CO 2 fixation reaction occurs at the oxaloacetate level. Both pyruvate carboxylase and phosphoenolpyruvate carboxykinase reactions lead to the formation of oxaloacetate, while the reaction of malic enzyme synthesizes malate.
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Journal of Biomaterials Science. Polymer Edition, 1999
A recognized drawback of the currently available chemical cross-linking reagents used to fix bioprostheses is the potential toxic effects a recipient may be exposed to from the fixed tissues and/or the residues.
Hsing-Wen Sung +3 more
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A recognized drawback of the currently available chemical cross-linking reagents used to fix bioprostheses is the potential toxic effects a recipient may be exposed to from the fixed tissues and/or the residues.
Hsing-Wen Sung +3 more
semanticscholar +1 more source
Fixation of Tissue for Histochemistry
1991The nature of the morphological information sought from any tissue dictates the fixation technique which is used. The first decision to be made is whether fixation will inhibit any subsequent technique to be used, for example, the use of some monoclonal antibodies in which case fixation will be postponed.
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Introduction to the Theory and Practice of Fixation of Tissues
Journal of Histotechnology, 2001Many approaches to fixation and types of fixatives have been developed and tested over the last-century.
Russell B. Myers +3 more
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The use of osmium in the fixation and staining of tissues
Proceedings of the Royal Society of London. Series B - Biological Sciences, 1957The use of gallic acid derivatives in the visualization of osmium in tissue sections has been re-investigated. By the use of alkyl esters of gallic acid greatly improved results can be obtained. Fixation with buffered osmium textroxide followed by ethyl gallate affords a simple and reliable method for staining fat droplets, mitochondria, etc. According
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