Unlike tobacco mosaic virus, which increases the respiration of tobacco leaves within an hour of their being inoculated, a virulent strain of tobacco etch virus did not change respiration rates until leaves showed external symptoms. The respiration rates of inoculated or systemically infected leaves with symptoms rose to 40% above that of healthy ...
Owen, P. C.
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An improved variant of tobacco etch virus (TEV) protease that does not need reducing agents [PDF]
Nikolaus Loening
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Unexpected tobacco etch virus (TEV) protease cleavage of recombinant human proteins [PDF]
Dominic Esposito
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CRISPR/Cas9-mediated editing of eukaryotic elongation factor 1B gamma (eEF1Bγ) reduces Tobacco etch virus accumulation in Nicotiana benthamiana [PDF]
Byoung-Cheorl Kang +2 more
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The P1′ specificity of tobacco etch virus protease [PDF]
Affinity tags have become indispensable tools for protein expression and purification. Yet, because they have the potential to interfere with structural and functional studies, it is usually desirable to remove them from the target protein. The stringent sequence specificity of the tobacco etch virus (TEV) protease has made it a useful reagent for this
Kapust, Rachel B. +3 more
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Turnip Mosaic Virus-Based gRNA Delivery System for Plant Genome Editing. [PDF]
ABSTRACT Plant virus‐based gRNA delivery systems offer a rapid alternative to stable transformation for CRISPR‐mediated genome editing, but potyvirus‐based platforms in Cas9‐expressing plants are still underexplored. Here, we developed a turnip mosaic virus (TuMV)‐based system for gRNA delivery in Cas9‐expressing Nicotiana benthamiana and tested ...
Khwanbua E +3 more
europepmc +2 more sources
Virus Diseases of Vegetable and Melon Crops in the South of the Russian Far East
Aim. The development of an inventory of phytoviruses affecting vegetable and melon crops in the South of the Russian Far East.Discussion. On the basis of many years of original research, carried out on a regular basis by the Laboratory of Virology ...
V. F. Tolkach +7 more
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Optimization of solvent media to solubilize TEV protease using response surface method
Background and purpose: Tobacco etch virus (TEV) protease, a 27 KDa protein, consists of the catalytic domain of nuclear inclusion a (NIa) protein produced by Tobacco etch virus.
Niloufar Mohammadian +5 more
doaj +1 more source
Rapid modification of proteins using a rapamycin-inducible tobacco etch virus protease system. [PDF]
The ability to disrupt the function of a specific protein on a rapid time scale provides a powerful tool for biomedical research. Specific proteases provide a potential method to selectively cleave a chosen protein, but rapid control of protease activity
Damian J Williams +2 more
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Engineering a temperature sensitive tobacco etch virus protease [PDF]
Since tobacco etch virus protease (TEVp) has a high specificity and efficiency in cleaving its target substrates, many groups have attempted to engineer conditional control of its activity. Temperature induction is widely used for modulating gene function because it has fast temporal response, good penetrability and applicability to many model ...
J, Wong, X, Chen, K, Truong
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