Results 31 to 40 of about 1,431,832 (205)
Large-Scale Purification of a Stable Form of Recombinant Tobacco Etch Virus Protease
BioTechniques, 2001 Tobacco etch virus NIa proteinase (NIa-Pro) has become the enzyme of choice for removing tags and fusion domains from recombinant proteins in vitro. We have designed a mutant NIa-Pro that resists autoproteolytic inactivation and present an efficient ...
Louise J. Lucast +2 more
doaj +1 more source
RNA packaging and uncoating in simple single-stranded RNA viruses [PDF]
, 2012 Simple (non-enveloped) small, positive-sense single-stranded RNA viruses infect hosts from all kingdoms of life. However, their assembly and uncoating processes remain poorly understood. For turnip crinkle virus (TCV), 3D reconstructions by cryoelectron
Bakker, Saskia
core +6 more sources
Molecular Plant-Microbe Interactions, 2013 Helper component proteinase (HCpro) is a multifunctional protein of potyviruses (genus Potyvirus). HCpro of Potato virus A (PVA) interacts with the microtubule-associated protein HIP2 in host cells, and depletion of HIP2 reduces virus accumulation.
Tuuli Haikonen +3 more
doaj +1 more source
Studia Universitatis Babes-Bolyai Chemia, 2022 Nowadays, protein purification by the aid of affinity tags can be carried out with high speed and efficiency. However, in several cases, affinity tags can significantly alter the key properties of enzymes, especially activity and/or thermostability ...
Alina FILIP +4 more
doaj +1 more source
Structural Basis for the Substrate Specificity of Tobacco Etch Virus Protease [PDF]
Journal of Biological Chemistry, 2002 Because of its stringent sequence specificity, the 3C-type protease from tobacco etch virus (TEV) is frequently used to remove affinity tags from recombinant proteins. It is unclear, however, exactly how TEV protease recognizes its substrates with such high selectivity.
Jason, Phan +8 more
openaire +2 more sources
Biologia Plantarum, 2013 Viral resistance can be effectively induced in transgenic plants through their silencing machinery. Thus, we designed nine short hairpin RNAs (shRNA) constructs to target nuclear inclusion protein b (NIb), helper component proteinase (HC-Pro ...
L. Zhang +5 more
doaj +1 more source
Virology Journal, 2023 Background To investigate the mechanism of RNA silencing suppression, the genetic transformation of viral suppressors of RNA silencing (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes the VSR variations caused by ...
Syuan-Fei Hong +10 more
doaj +1 more source
A multidirectional non-cell autonomous control and a genetic interaction restricting tobacco etch virus susceptibility in Arabidopsis. [PDF]
PLoS ONE, 2007 Viruses constitute a major class of pathogens that infect a variety of hosts. Understanding the intricacies of signaling during host-virus interactions should aid in designing disease prevention strategies and in understanding mechanistic aspects of host
Suresh Gopalan
doaj +1 more source
Frontiers in Immunology, 2021 We have developed a new binary epitope-presenting CVP platform based on bamboo mosaic virus (BaMV) by using the sortase A (SrtA)-mediated ligation technology. The reconstructed BaMV genome harbors two modifications: 1) a coat protein (CP) with N-terminal
Ming-Hao Yang +11 more
doaj +1 more source
Journal of Ultrastructure Research, 1974 Structural subunits of the crystalline inclusion induced by tobacco etch virus were clearly resolved when inclusions were examined both in situ and in isolated preparations by freeze-etch electron microscopy. Data supported the contention that the crystal substructure is organized into laminae and that these laminae assemble into crystals by stacking
Plant Pathology Department, Plant Virus Laboratory, University of Florida, Gainesville, Florida 32611, USA ( host institution ) +2 more
openaire +3 more sources