Results 61 to 70 of about 1,431,832 (205)

Proteins synthesized in tobacco mosaic virus infected protoplasts [PDF]

, 1979
The study described here concerns the proteins, synthesized as a result of tobacco mosaic virus (TMV) multiplication in tobacco protoplasts and in cowpea protoplasts. The identification of proteins involved in the TMV infection, for instance in the virus
Huber, R.
core  

OPTIMIZATION OF METHODS FOR PREPARATION OF RECOMBINANT TOBACCO ETCH VIRUS PROTEASE FROM Escherichia coli cells

Сибирский научный медицинский журнал, 2019
The catalytic domain of the nuclear inclusion protein of the tobacco etch virus protease, TEVp, is used for the cleavage of artificial fusion polypeptides.
A. V. Ryabchenko, M. V. Kotova, R. A. Knyazev, N. V. Trifonova, L. M. Polyakov   +4 more
doaj   +1 more source

AS‐TLC‐Based High‐Throughput Screening Integrated with CuAAC Optimization Strategy Facilitates the Rapid Discovery of YTHDC1 Inhibitor

Advanced Science, EarlyView.
We developed Affinity Selection Thin‐Layer Chromatography (AS‐TLC) as a method for high‐throughput screening (HTS) of compound mixtures. Furthermore, we integrated activity assays with AS‐TLC and identified a YTH domain‐containing protein 1 (YTHDC1) inhibitor. Building on this hit, we applied copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC) click
Mingchen Wang, Shuiping Fu, Ye Xu, Jia Gao, Jiwei Ren, Xu Yang, Hesong Xu, Ge Sun, Yanjie Li, Yanlin Wang, Lu Jin, Peijuan Chen, Ying Xu, Jianhao Li, Hua Lin, Chujiao Hu, Kaixian Chen, Huan Xiong, Cheng Luo, Shijie Chen   +19 more
wiley   +1 more source

Immobilized Triton X-100-assisted refolding of Green Fluorescent Protein-Tobacco Etch Virus protease fusion protein using beta-cyclodextrin as the eluent

, 2009
A new protein refolding technique based on the use of the non-charged detergent Triton X-100 immobilized to the cross-linked agarose gel Sepharose High Performance has been developed.
Janson, Jan-Christer, Su, ZG, Ballagi, Andras,, Li, Jing Jing, Janson, Jan-Christer,, Ballagi, Andras, Wang, Ai-Qing, Su, Zhi-Guo, Liu, Yong-Dong, Li, Jing-Jing, Chen, Jing, Ma, Guang-Hui   +11 more
core   +1 more source

Identification and engineering of highly functional potyviral proteases in cells using co-evolutionary models

Nature Communications
Efficiency and substrate specificity of proteases in the Potyviridae family have not been comprehensively profiled. Here we develop a model that learns co-evolutionary features to accurately predict and experimentally validate protease performance at ...
Medel B. Lim Suan, Cheyenne Ziegler, Zain Syed, Arjun Sai Yedavalli, Jaimahesh Nagineni, Rodrigo Raposo, Ajay Tunikipati, Jaideep Kaur, Faruck Morcos, P. C. Dave P. Dingal   +9 more
doaj   +1 more source

Stability of Tobacco etch virus infectious clones in plasmid vectors

Virus Research, 2010
Tobacco etch virus (TEV) has been traditionally used as a model to research many aspects of the molecular biology of plant RNA virus and, more recently, experimental evolution. However, the only plasmid of this virus species with an infectious clone that has been commonly available to research (pTEV7DA) is rather unstable when propagated in the ...
Leonor C, Bedoya, José-Antonio, Daròs
openaire   +2 more sources

Engineered Transformer Base Editor with Enhanced Editing Efficiency

Advanced Science, EarlyView.
A highly efficient transformer base editor system achieves robust genomic editing in a humanized mouse model. This work establishes a versatile and translatable platform, opening new avenues for precision gene therapy. ABSTRACT Canonical cytosine base editors (CBEs) achieve precise C‐to‐T conversions without inducing DNA double‐strand breaks (DSBs ...
Bowen Chen, Letong Liang, Rui Xu, Tianyi Wang, Hongyan Chen, Daru Lu   +5 more
wiley   +1 more source

Plain Tobacco Packaging: A Systematic Review [PDF]

, 2012
(From the Executive Summary): This systematic review outlines findings from 37 studies that provide evidence of the impacts of plain tobacco packaging.
Hinds, Kate, O’Mara-Eves, Alison, Stead, M, Hastings, Gerard, O'Mara-Eves, Alison, Bauld, Linda, Stead, Martine, Kwan, I, McNeill, Ann, Hinds, K, Thomas, James, Hastings, G, Moodie, C, Bauld, L, Kwan, Irene, Thomas, J, Angus, K, Angus, Kathryn, McNeill, A, Moodie, Crawford, O'Mara-Eves, A   +20 more
core  

Heterologous expression of plant virus genes that suppress post-transcriptional gene silencing results in suppression of RNA interference in Drosophila cells

BMC Biotechnology, 2004
Background RNA interference (RNAi) in animals and post-transcriptional gene silencing (PTGS) in plants are related phenomena whose functions include the developmental regulation of gene expression and protection from transposable elements and viruses ...
Canto Tomas, Dawson Sheila, Reavy Brian, MacFarlane Stuart A   +3 more
doaj   +1 more source

Hybrid Macrocyclic Peptides — Synthetic Strategies to Diversify and Cyclize Peptide Libraries in Phage Display Selections

Chemistry – A European Journal, EarlyView.
Hybrid macrocyclic peptides unite genetically encoded peptide libraries with the structural complexity of synthetic small molecules. This Review critically analyzes phage‐compatible cyclization and diversification chemistries, highlights emerging opportunities beyond traditional cysteine‐based approaches, and outlines how future advances may enable the
Titia Rixt Oppewal, Clemens Mayer
wiley   +1 more source