Results 71 to 80 of about 1,431,832 (205)

Advancing large-scale production of TEV protease through an innovative NT* tag-based fusion construct

open access: yesCurrent Research in Structural Biology, 2023
Tobacco etch virus Protease (TEVp), a cysteine protease, is renowned for its remarkable specific proteolysis, making it an invaluable tool for removing fusion tags from recombinant proteins.
Pragyan P. Parida   +3 more
doaj   +1 more source

Structural and Biochemical Characterization of the Diaminopimelate Epimerase DapF From Pseudomonas aeruginosa PA14

open access: yesProteins: Structure, Function, and Bioinformatics, EarlyView.
ABSTRACT The emergence of antibiotic resistance in bacteria is a major threat to global public health. To combat increasingly drug‐resistant bacteria like Pseudomonas aeruginosa, new targets for pharmaceuticals need to be explored. One such potential target for P.
Philipp Schneider   +4 more
wiley   +1 more source

Acetylsalicylic-acid (aspirin) induces resistance to tobacco mosaic-virus in tobacco

open access: yes, 1979
Tobacco cv. Xanthi-nc becomes resistant to tobacco mosaic virus (TMV) and produces b proteins when injected with the interferon inducer polyacrylic acid.
White, R. F.
core   +1 more source

Partial purification of inclusions induced by tobacco etch virus and potato virus Y

open access: yesVirology, 1971
Abstract Virus-induced inclusions were partially purified from tobacco plants infected with tobacco etch virus (TEV) or potato virus Y (PVY). Tissue was homogenized in phosphate buffer containing urea. The homogenate was treated with Triton X-100, followed by differential centrifugation, homogenization, filtration, and sucrose density-gradient ...
Department of Plant Pathology, University of Florida, Gainesville, Florida 32601 USA ( host institution )   +4 more
openaire   +3 more sources

Single and dual RPA‐CRISPR/Cas assays for point‐of‐need detection of Stewart's wilt pathogen (Pantoea stewartii subsp. stewartii) of corn and Maize dwarf mosaic virus

open access: yesPest Management Science, Volume 81, Issue 4, Page 1988-1999, April 2025.
Schematic diagram of the single and dual RPA‐CRISPR/Cas12a/13a diagnostic assays for the detection of Pantoea stewartii subsp. stewartii and Maize dwarf mosaic virus. The validated assays provide a useful and sensitive molecular tool for detecting two quarantine pathogens of maize within a minimal resource framework suitable for fast‐tracking the ...
Qian Tian   +6 more
wiley   +1 more source

Obtenção de um biocatalisador da protease TEV para a remoção de caudas de histidinas de proteínas recombinantes [PDF]

open access: yes, 2013
Dissertação (mestrado) - Universidade Federal de Santa Catarina. Programa de Pós-graduação em BiotecnologiaO uso de caudas de afinidade tem facilitado a purificação de proteínas recombinantes para aplicações bioquímicas, terapêuticas e estudos ...
Puhl, Ana Cristina
core  

Mammalian expression vectors for metabolic biotinylation tandem affinity tagging by co-expression in cis of a mammalian codon-optimized BirA biotin ligase

open access: yesBMC Research Notes, 2018
Οbjective To construct mammalian expression vectors for the N- or C-terminal tagging of proteins with a tandem affinity tag comprised of the biotinylatable Avi tag and of a triple FLAG tag.
Marina Ioannou   +3 more
doaj   +1 more source

Kinetics of Bovine leukemia virus aspartic protease reveals its dimerization and conformational change.

open access: yesPLoS ONE, 2022
The retropepsin (PR) of the Bovine leukemia virus (BLV) plays, as in other retroviruses, a crucial role in the transition from the non-infective viral particle to the infective virion by processing the polyprotein Gag.
Martín Fló   +7 more
doaj   +1 more source

Alternative [2Fe‐2S] donor assemblies in the cytosol: GLRX3 and GLRX3‐BOLA2 converge on NUBP1 maturation

open access: yesThe FEBS Journal, EarlyView.
GLRX3 homodimer and GLRX3‐BOLA2 heterotrimer act as alternative [2Fe‐2S] donor assemblies for NUBP1 maturation. Both systems support formation of [4Fe‐4S] cluster‐bound NUBP1, but display distinct kinetic dependence on the physiological electron donor anamorsin, which accelerates GLRX3‐mediated transfer but does not enhance GLRX3‐BOLA2‐mediated ...
Martina Masini   +2 more
wiley   +1 more source

Rescue of pATOM36‐depleted T. brucei by human MTCH1/2 reveals common features of protein insertases

open access: yesThe FEBS Journal, EarlyView.
Human MTCH1 and MTCH2 (with an N‐terminal extension) functionally complement the loss of pATOM36 in T. brucei, indicating that these evolutionarily distant proteins recognize a common set of substrates and share fundamental principles of mitochondrial outer membrane protein insertion. Structural analyses reveal a common hydrophilic cavity, supporting a
Stephan Berger   +4 more
wiley   +1 more source

Home - About - Disclaimer - Privacy