Results 61 to 70 of about 28,220 (244)
Tobacco Rattle Virus in Tobacco Mesophyll Protoplasts: Infection and Virus Multiplication
Journal of General Virology, 1975 Summary Poly-l-ornithine greatly increased infection of protoplasts by tobacco rattle virus (TRV), and had the largest effect when incubated with virus particles for at least 10 min before inoculation. Using a final concentration of 1 µg/ml TRV particles and 1 to 1.5 µg/ml poly-l-ornithine in 0.025 m-phosphate buffer, pH 6.0, to inoculate mesophyll ...
S. Kubo +3 more
openaire +1 more source
, 2021 Virus-induced gene silencing (VIGS) is a fast and efficient functional genomics tool (Becker and Lange, 2009; Baulcombe, 1999) and a method for rapid silencing of plant genes in order to discover their function (Becker and Lange, 2009; Purkayastha and ...
Gyan Prakash Srivastav Shilpy Singh +2 more
semanticscholar +1 more source
Efficient Protein Expression and Virus-Induced Gene Silencing in Plants Using a Crinivirus-Derived Vector. [PDF]
, 2018 Plant virus-based vectors are valuable tools for recombinant gene expression and functional genomics for both basic and applied research. In this study, Lettuce infectious yellows virus (LIYV) of the genus Crinivirus was engineered into a virus vector ...
Falk, Bryce W, Qiao, Wenjie
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Effect of green manure crops and organic amendments on incidence of nematode-borne tobacco rattle virus [PDF]
Tobacco rattle tobravirus (TRV) may infect several ornamental bulb crops and is transmitted by trichodorid nematodes. Paratrichodorus teres, P. pachydermus and Trichodorus similis are the main vectors in the Netherlands.
Asjes, C.J. +4 more
core +2 more sources
Molecular Plant-Microbe Interactions, 2016 Field-grown tubers of potato were examined for infection by Tobacco rattle virus (TRV) and consequent production of corky ringspot or spraing symptoms. A microarray study identified genes that are differentially expressed in tuber tissue in response to ...
Ghulam Sahi +4 more
doaj +1 more source
BMC Plant Biology, 2021 Background Virus-induced gene silencing (VIGS) is one of the most convenient and powerful methods of reverse genetics. In vitro-inoculation of plant virus is an important method for studying the interactions between viruses and plants.
Qili Liu +16 more
doaj +1 more source
Physical methods for soil disinfestation in intensive agriculture: Old methods and new approaches [PDF]
, 2010 Physical soil disinfestation is worldwide mainly applied in protected cropping systems or in small-scale intensive field crops. Continuous cropping of monocultures or different host plants for the same pest or pathogen often leads to heavily infested ...
Molendijk, L.P.G., Runia, W.T.
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An improved cucumber mosaic virus-based vector for efficient decoying of plant microRNAs. [PDF]
, 2015 We previously devised a cucumber mosaic virus (CMV)-based vector system carrying microRNA target mimic sequences for analysis of microRNA function in Arabidopsis thaliana.
Carr, John P +3 more
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Enhancer Trapping Identifies TRI, an Arabidopsis Gene Up-Regulated by Pathogen Infection
Molecular Plant-Microbe Interactions, 2004 Enhancer trap Arabidopsis thaliana plants were screened for genes up-regulated by virus infection. The plants carried T-DNA insertions comprising a minimal -60-bp Cauliflower mosaic virus 35S promoter fused to the β-glucuronidase (GUS) reporter gene ...
Ingela Fridborg +5 more
doaj +1 more source
Frontiers in Plant Science, 2022 RNA silencing is a common antiviral mechanism in eukaryotic organisms. However, the transcriptional regulatory mechanism that controls the RNA silencing process remains elusive.
Yingru Xu +9 more
doaj +1 more source