Results 81 to 90 of about 643,132 (282)
FEBS Letters, EarlyView.We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee +3 more
wiley +1 more source
FEBS Letters, EarlyView.We identified a systemic, progressive loss of protein S‐glutathionylation—detected by nonreducing western blotting—alongside dysregulation of glutathione‐cycle enzymes in both neuronal and peripheral tissues of Taiwanese SMA mice. These alterations were partially rescued by SMN antisense oligonucleotide therapy, revealing persistent redox imbalance as ...
Sofia Vrettou, Brunhilde Wirth
wiley +1 more source
Evaluation of a semi-automatic isoelectric focusing method for apolipoprotein E phenotyping [PDF]
, 2020 International audienceA qualitative, semi-automatized method for apolipoprotein E (apoE) phenotyping by isoelectric focusing method has been evaluated on 40 serum samples from patients previously genotyped for apoE, especially as regards concordance with
Cherfils, Corinne +7 more
core +1 more source
FEBS Letters, EarlyView.In this study, we found that human cervical‐derived adipocytes maintain intracellular iron level by regulating the expression of iron transport‐related proteins during adrenergic stimulation. Melanotransferrin is predicted to interact with transferrin receptor 1 based on in silico analysis.
Rahaf Alrifai +9 more
wiley +1 more source
e-Journal of Nondestructive TestingThe Total Focusing Method (TFM) technique is an advanced Non-Destructive Testing (NDT) Ultrasonic Testing (UT) technique. It is usually used together with special acquisition configurations such as Full Matrix Capture (FMC) or Plane Wave Imaging (PWI ...
Wang Ke, Weina Ke
doaj +1 more source
Tau acetylation at K331 has limited impact on tau pathology in vivo
FEBS Letters, EarlyView.We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto +3 more
wiley +1 more source
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
e-Journal of Nondestructive TestingThe total focusing method (TFM) is increasingly recognized as a powerful ultrasonic imaging technique for the quality assessment and internal defect detection of carbon fiber-reinforced composites (CFRPs).
Jiancheng Cao +3 more
doaj +1 more source
Rician Beamforming: Despeckle Method via Coarray Projection Stochastic Analysis
Applied Sciences, 2020 Current computation capabilities normal, Delay and Sum (DAS) and Total Focusing Method (TFM) allow new definitions for beamformers. In this paper, a new beamforming method is proposed. It exploits diversity across pixel data after focusing process.
Virginia Yagüe-Jiménez +3 more
doaj +1 more source
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source