Results 191 to 200 of about 11,033,420 (264)

Advances in Unraveling the Structure and Sodium‐Ion Storage Behaviors of Hard Carbons: From Basic to Extended Characterization Techniques

Carbon Energy, EarlyView.
This review is devoted to introducing the structures and sodium storage mechanisms of hard carbon materials and analyzing the correlated advanced characterization techniques. The advanced characterization techniques are categorized based on the classification of signal sources.
Jiechang Gao, Dongqi Zhang, Guanglei Qiu, Ye Han, Haijun Zhang, Yajie Liu, Zhixin Tai   +6 more
wiley   +1 more source

Preparation of filamentous actin for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays. [PDF]

Cold Spring Harb Protoc, 2012
Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE.   +4 more
europepmc   +1 more source

Formic‐Acid In Situ Hydrogen Enables Programmable Ru–Pd–Pt/C Trimetallic Interfaces for Durable Acidic HER

Carbon Energy, EarlyView.
Bamboo diaphragm−derived carbon is used as a support to prepare Ru–Pd–Pt/C catalysts through a stepwise reduction strategy. The Ru–Pd interface promotes formic acid activation and induces Pt deposition. The metallic interface of Ru–Pd–Pt/C enables H* generation, transfer, and recombination, thereby improving Pt utilization, acidic HER activity, and PEM
Liangmeng Ni, Aiyue Huang, Xin Feng, Zhijia Liu   +3 more
wiley   +1 more source

Nanotopology of cell adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy (VA-TIRFM). [PDF]

J Vis Exp, 2012
Wagner M, Weber P, Baumann H, Schneckenburger H.   +3 more
europepmc   +1 more source

Purification, Fluorescent Labeling, and Detyrosination of Mammalian Cell Tubulin for Biochemical Assays

Cytoskeleton, EarlyView.
ABSTRACT Microtubules play essential roles in numerous cellular processes. All microtubules are built from the protein tubulin, yet individual microtubules can differ spatially and temporally due to their tubulin isotype composition and post‐translational modifications (PTMs).
Ezekiel C. Thomas, Yang Yue, Morgan L. Pimm, Takashi Hotta, Ryoma Ohi, Kristen J. Verhey   +5 more
wiley   +1 more source

Protocol for Reconstituting Adaptor‐Mediated Activation of Full‐Length Kinesin‐1

Cytoskeleton, EarlyView.
ABSTRACT Kinesin‐1 is a member of the kinesin superfamily that plays an essential role in intracellular cargo transport. In the absence of cargo, Kinesin‐1 exhibits low motor activity due to autoinhibition. Multiple studies have demonstrated that adaptor proteins, which link cargos to Kinesin‐1, can activate Kinesin‐1 by releasing the autoinhibition ...
Haruka Masumoto, Kyoko Chiba
wiley   +1 more source

Orientation and rotational motions of single molecules by polarized total internal reflection fluorescence microscopy (polTIRFM). [PDF]

Cold Spring Harb Protoc, 2012
Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE.   +4 more
europepmc   +1 more source

Engineering and Characterization of a Fluorescent Fission Yeast Arp2/3 Complex for Single Molecule Mechanistic Investigations

Cytoskeleton, EarlyView.
ABSTRACT Arp2/3 complex is a seven‐component protein complex that facilitates the assembly of branched actin filament networks by binding to pre‐existing “mother” actin filaments and initiating the nucleation of new branched “daughter” filaments. Arp2/3 complex must be activated by a nucleation promoting factor such as the WASP/Scar protein family. The
Caitlin A. Anderson, Meghan E. O'Connell, Kaitlin E. Homa, Cristian Suarez, Michael L. James, Kashmeera D. Baboolall, Vilmos Zsolnay, Vladimir Sirotkin, David R. Kovar   +8 more
wiley   +1 more source

Fluorescent labeling of myosin V for polarized total internal reflection fluorescence microscopy (polTIRFM) motility assays. [PDF]

Cold Spring Harb Protoc, 2012
Beausang JF, Sun Y, Quinlan ME, Forkey JN, Goldman YE.   +4 more
europepmc   +1 more source

Morphological signatures of bacteria‐laden drying droplets: A multimodal analysis across host fluids

Droplet, EarlyView.
Drying patterns of bacteria‐laden droplets encode concentration‐dependent signatures across three host fluids: serum, urine, and nutrient medium. By integrating time‐evolving texture descriptors, image features, and interpretable multimodal machine learning, this study demonstrates a label‐free approach for extracting information on bacterial ...
Anusuya Pal, Amalesh Gope, Piyush Thakur, Gen Honda, Emi Hada, Satoshi Sawai, Miho Yanagisawa   +6 more
wiley   +1 more source