Results 101 to 110 of about 3,562,641 (311)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Dietary diversity score and the acceleration of biological aging: a population-based study of 88,039 participants

The Journal of Nutrition, Health and Aging
Objectives: Our study aimed to investigate the association of dietary diversity score (DDS), as reflected by five dietary categories, with biological age acceleration. Design: A cross-sectional study.
Ye Liu, Meijuan Kang, Wenming Wei, Jingni Hui, Yifan Gou, Chen Liu, Ruixue Zhou, Bingyi Wang, Panxing Shi, Huan Liu, Bolun Cheng, Yumeng Jia, Yan Wen, Feng Zhang   +13 more
doaj   +1 more source

Cellular responses to T-2 toxin and/or deoxynivalenol that induce cartilage damage are not specific to chondrocytes

Scientific Reports, 2017
The relationship between T-2 toxin and deoxynivalenol (DON) and the risk of Kashin-Beck disease is still controversial since it is poorly known about their selectivity in cartilage damage. We aimed to compare the cytotoxicity of T-2 toxin and DON on cell
Yang Lei, Zhao Guanghui, Wang Xi, Wang Yingting, Lin Xialu, Yu Fangfang, Mary B. Goldring, Guo Xiong, Mikko J. Lammi   +8 more
doaj   +1 more source

Trace elements of botton water samples of cruise SO129

, 2001
Trace elements of botton water samples of cruise ...
Matthias Zabel (6886103), Lars Dittert (6972041), Dittert, Lars, Zabel, Matthias   +3 more
core   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Trace elements of sediment core SO129_MC-12

, 2001
Trace elements of sediment core SO129_MC ...
Matthias Zabel (6886103), Lars Dittert (6972041), Dittert, Lars, Zabel, Matthias   +3 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Association between intake of various sugar subtypes and common mental disorders: A large prospective study

The Journal of Nutrition, Health and Aging
Objective: Sugar is an essential part of the daily diet but poses multiple health risks. Previous studies have neglected the association between specific sugar subtypes and mental health.
Lina Qin, Boyue Zhao, Meijuan Kang, Tingting Mao, Weixuan Da, Yue Che, Hanchi Wang, Yijia Li, Jin Feng, Yifan Gou, Li Liu, Huan Liu, Bolun Cheng, Yumeng Jia, Yan Wen, Feng Zhang   +15 more
doaj   +1 more source

Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank

FEBS Open Bio, EarlyView.
Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer, Pawel Rubach, Zbigniew Dauter, Wojciech Dec, Dariusz Brzezinski, Marta Kulik, Wladek Minor, Mariusz Jaskolski   +7 more
wiley   +1 more source

A minimal cellulosome‐like system in Cellulosilyticum lentocellum

FEBS Open Bio, EarlyView.
Cellulose‐degrading bacteria typically use cellulosomes, large multi‐enzyme complexes on a scaffold protein. In Cellulosilyticum lentocellum, we characterise a far smaller arrangement, a single scaffold bound to one cellulase through a single cohesin‐dockerin interaction.
John Allan, Amias Alstrom‐Moore, Gary W Black   +2 more
wiley   +1 more source