Results 81 to 90 of about 368,022 (299)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Does depleted mantle form an intrinsic part of the Iceland plume? [PDF]

, 2003
Icelandic basalt ranges in composition from voluminous tholeiite, erupted in the rift zones, to small-volume, mildly alkaline basalt erupted off-axis.
Fitton, J. G., Hardarson, B. S., Kempton, P. D., Saunders, A. D.   +3 more
core   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

Amygdala-hippocampal involvement in human aversive trace conditioning revealed through event-related functional magnetic resonance imaging

, 1999
Previous functional neuroimaging studies have characterized brain systems mediating associative learning using classical delay conditioning paradigms.
Buchel, C, Friston, K., Armony, J., Armony, J, Dolan, R, Dolan, R. ; https://orcid.org/, Buchel, C., Friston, K   +7 more
core  

State-trace analysis: dissociable processes in a connectionist network? [PDF]

, 2014
This is the peer reviewed version of the following article: Yeates, F., Wills, A. J., Jones, F. W. and McLaren, I. P. L. (2015), State-Trace Analysis: Dissociable Processes in a Connectionist Network?.
McLaren, Ian P.L., Wills, A., Jones, FW, Yeates, F., Jones, F., Jones, Fergal W., Yeates, Fayme, Wills, AJ, McLaren, I., Wills, Andy J.   +9 more
core   +1 more source

Organellar microcapture to extract nuclear and plastid DNA from recalcitrant wood specimens and trace evidence. [PDF]

Plant Methods, 2022
Costa A, Giraldo G, Bishell A, He T, Kirker G, Wiedenhoeft AC.   +5 more
europepmc   +1 more source

Odour as trace evidence

Journal of Education Culture and Society, 2020
There are a number of scent classification systems that typically take into account the similarities and differences in human olfactory perception, regardless of what chemical compounds cause a particular sensation. Also there is no objective, physical or chemical basis for the classification of odours, unlike eyesight or hearing.
openaire   +2 more sources

Cyclic azapeptide CD36 ligand attenuates cardiac injury and reduces long‐chain fatty acid accumulation after myocardial ischemia–reperfusion in mice

FEBS Open Bio, EarlyView.
In a murine model of myocardial ischemia and reperfusion (MI/R), the CD36 azapeptide ligand MPE‐298 reduces cardiac injury and transiently lowers left ventricular long‐chain fatty acids (LCFAs) accumulation 3 h after reperfusion, accompanied by a decrease of oxidative stress and inflammation‐associated genes' expression in the heart and adipose tissue.
Jade Gauvin, Naghme Radmannia, David N. Huynh, Liliane Ménard, Caroline Daneault, Maïté Veilleux, Ahsanullah Ahsanullah, André C. Carpentier, William D. Lubell, Matthieu Ruiz, Huy Ong, Sylvie Marleau, Simon‐Pierre Gravel   +12 more
wiley   +1 more source

Evaluation of low trace DNA recovery techniques from ridged surfaces

, 2013
The first step in the DNA profiling process is the recovery of DNA from the crime scene or item. Such surfaces can be varying with absorbent and non-absorbent surfaces.
Williams, Graham, Ahmed, Waseeh, Pandre, Manohar, Omelia, Emma J., Beasley, Emma   +4 more
core   +1 more source