Results 81 to 90 of about 42,852 (256)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Metrological traceability chain for PCBs: INRIM activity

open access: yes, 2008
Metrology in chemistry has its own features, which distinguish it from classical metrology: due to the lack of primary methods applicable in routine measurements, metrological traceability of measurement results can be achieved by using in a proper way ...
SEGA, MICHELA   +2 more
core   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Buyer-supplier relationships influence on traceability implementation in the vegetable industry [PDF]

open access: yes, 2006
The increasing importance of food safety has made traceability a crucial issue in the agri-business industry. In this article, we have analysed the factors that shape the buyer-supplier relationships, and how they influence the traceability of raw ...
Alfaro, José A.   +3 more
core  

Safety Risk Assessment and Classification of Cadmium in Grain Processing Products

open access: yesFoods
As a staple food for people worldwide, processed grain products, including rice, wheat flour, etc., have been the major source of the toxic element cadmium for human exposure.
Qingchuan Zhang   +4 more
doaj   +1 more source

Fast Simultaneous Determination of Eight Sudan Dyes in Chili Oil by Ultra-High-Performance Supercritical Fluid Chromatography

open access: yesJournal of Analytical Methods in Chemistry, 2019
A rapid and simple ultra-high-performance supercritical fluid chromatography (UHPSFC) with photodiode array (PDA) method was developed and validated for simultaneous determination of eight Sudan dyes in chili oil.
Yunjia Yang   +3 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Traceability

open access: yesChallenging Glass Conference Proceedings
Achieving a circular business model for recovering building materials needs multi-level actions within the construction chain and strengthen the stakeholder engagement. One actual trade back in the reclamation of building materials is the lack of comprehensive data and information regarding their history and features.
Angelica Rota   +2 more
openaire   +3 more sources

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

open access: yesFEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata   +13 more
wiley   +1 more source

Rapid Screening for Hazardous Substances with Regulatory Differences in Milk Between Countries Using Ultra-High Performance Liquid Chromatography Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry

open access: yesFoods
Regulatory heterogeneity on the maximum residue levels (MRLs) of hazardous substances for food is identified as a challenge of trade between countries. To balance the import and export trade of milk, a high-throughput determination method was established
Qiaozhen Guo   +5 more
doaj   +1 more source

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