Results 71 to 80 of about 38,375 (219)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Rapid Screening for Hazardous Substances with Regulatory Differences in Milk Between Countries Using Ultra-High Performance Liquid Chromatography Ion Mobility Quadrupole Time-of-Flight Mass Spectrometry

Foods
Regulatory heterogeneity on the maximum residue levels (MRLs) of hazardous substances for food is identified as a challenge of trade between countries. To balance the import and export trade of milk, a high-throughput determination method was established
Qiaozhen Guo, Jing Zhang, Bing Shao, Jie Yin, Yunjia Yang, Yi Yang   +5 more
doaj   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Fast Simultaneous Determination of Eight Sudan Dyes in Chili Oil by Ultra-High-Performance Supercritical Fluid Chromatography

Journal of Analytical Methods in Chemistry, 2019
A rapid and simple ultra-high-performance supercritical fluid chromatography (UHPSFC) with photodiode array (PDA) method was developed and validated for simultaneous determination of eight Sudan dyes in chili oil.
Yunjia Yang, Jing Zhang, Jie Yin, Yi Yang   +3 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

AASNet: A Novel Image Instance Segmentation Framework for Fine-Grained Fish Recognition via Linear Correlation Attention and Dynamic Adaptive Focal Loss

Applied Sciences
Smart fisheries, integrating advanced technologies such as the Internet of Things (IoT), artificial intelligence (AI), and image processing, are pivotal in enhancing aquaculture efficiency, sustainability, and resource management by enabling real-time ...
Jianlei Kong, Shunong Tang, Jiameng Feng, Lipo Mo, Xuebo Jin   +4 more
doaj   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

Argonaute combined with isothermal amplification for simultaneous detection of Vibrio parahaemolyticus and the tetracycline resistance gene tetA in water and food samples

Current Research in Food Science
V. parahaemolyticus is one of the most important foodborne pathogens and poses a serious threat to public health because of its high incidence and transferable antimicrobial resistance genes (ARGs).
Haibo Zhou, Jun Yang, Feng Xue, Wei Shen, Yiyu Cheng, Xinmei Liu   +5 more
doaj   +1 more source

Sudden anaerobization in Amphibacillus xylanus increases intracellular labile ferrous iron and inhibits cell growth

FEBS Open Bio, EarlyView.
Abruptly changing from aerobic to anaerobic conditions (sudden anaerobization) induced growth inhibition and a significant increase in intracellular labile ferrous iron in the aerotolerant anaerobe Amphibacillus xylanus. We found that free flavins mediate efficient electron transfer from NADH to ferric iron under anaerobic conditions, suggesting that ...
Shinya Kimata, Yoichi Sakai, Keisuke Tanaka, Etsuro Yoshimura, Ken Kitano, Morio Ishikawa, Tadahiro Nakamoto, Tsutomu Takayama, Hirofumi Inoue, Kaho Nomura, Tomonori Suzuki, Masataka Uchino, Akira Abe, Youichi Niimura   +13 more
wiley   +1 more source

DNA promoter task-oriented dictionary mining and prediction model based on natural language technology

Scientific Reports
Promoters are essential DNA sequences that initiate transcription and regulate gene expression. Precisely identifying promoter sites is crucial for deciphering gene expression patterns and the roles of gene regulatory networks.
Ruolei Zeng, Zihan Li, Jialu Li, Qingchuan Zhang   +3 more
doaj   +1 more source