Results 81 to 90 of about 6,801,451 (249)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Open Geosciences, 2015 The aim of this paper is to evaluate the vulnerabilityafter point source contamination and characterizewater circulations in volcanic flows located in theArgnat basin volcanic system (Chaîne des Puys, FrenchMassif Central) using a tracer test performed ...
Bertrand G. +8 more
doaj +1 more source
PCS-Q-G. Parental Caregiving Style Questionnaire – German Version
Der PCS-Q-G dient der retrospektiven Erfassung des elterlichen Fürsorgeverhaltens. In Analogie zum 3-Kategorien-Modell von Bindung unterscheidet das Instrument zwischen den drei Stilen „warm/aufmerksam“, „ambivalent/inkonsistent“ und „kalt/abweisend ...
Rohmann, E., Neumann, E.
core +1 more source
Tracer Dispersion in Rough Open Cracks [PDF]
, 1998 Tracer dispersion is studied in an open crack where the two rough crack faces have been translated with respect to each other. The different dispersion regimes encountered in rough-wall Hele-Shaw cell are first introduced, and the geometric dispersion ...
Plouraboué, Franck +2 more
core +1 more source
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
FEBS Open Bio, EarlyView.Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty +13 more
wiley +1 more source
STARC-5. Fragebogen für Empowerment und Resilienz
STARC-5 eignet sich mit 5 analytisch entwickelten Dimensionen (Self-esteem/ Selbstvertrauen, Trust/Optimismus, Affective resilience/Affektive Resilienz, Reliable problem solving/Problemlösekompetenz, Cognitive strength/Kognitive Stärke) und insgesamt 39 ...
Himmer-Gurdan, S. B.
core +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source