Coupling traction force patterns and actomyosin wave dynamics reveals mechanics of cell motion
Molecular Systems Biology, 2021 Motile cells can use and switch between different modes of migration. Here, we use traction force microscopy and fluorescent labeling of actin and myosin to quantify and correlate traction force patterns and cytoskeletal distributions in Dictyostelium ...
Elisabeth Ghabache +5 more
doaj +4 more sources
3D Traction Force Microscopy in Biological Gels: From Single Cells to Multicellular Spheroids. [PDF]
Annu Rev Biomed EngCell traction force plays a critical role in directing cellular functions, such as proliferation, migration, and differentiation. Current understanding of cell traction force is largely derived from 2D measurements where cells are plated on 2D substrates.
Cheung BCH, Abbed RJ, Wu M, Leggett SE.
europepmc +4 more sources
Spatiotemporally Super-Resolved Volumetric Traction Force Microscopy [PDF]
Nano Letters, 2019 Quantification of mechanical forces is a major challenge across biomedical sciences. Yet such measurements are essential to understanding the role of biomechanics in cell regulation and function. Traction force microscopy remains the most broadly applied
Huw Colin-York +13 more
semanticscholar +7 more sources
Combined Traction Force-Atomic Force Microscopy Measurements of Neuronal Cells. [PDF]
Biomimetics (Basel), 2022 In the course of the development of the nervous system, neuronal cells extend (grow) axons, which navigate over distances of the order of many cell diameters to reach target dendrites from other neurons and establish neuronal circuits.
Kumarasinghe U, Fox LN, Staii C.
europepmc +2 more sources
Migration and 3D Traction Force Measurements inside Compliant Microchannels. [PDF]
Nano Lett, 2022 Cells migrate in vivo through channel-like tracks. While polydimethylsiloxane devices emulate such tracks in vitro, their channel walls are impermeable and have supraphysiological stiffness.
Afthinos A +10 more
europepmc +2 more sources
Validation tool for traction force microscopy
Computer Methods in Biomechanics and Biomedical Engineering, 2014 Traction force microscopy (TFM) is commonly used to estimate cells' traction forces from the deformation that they cause on their substrate. The accuracy of TFM highly depends on the computational methods used to measure the deformation of the substrate and estimate the forces, and also on the specifics of the experimental set-up.
Álvaro Jorge-Peñas +3 more
+8 more sources
Traction force microscopy by deep learning [PDF]
Biophysical Journal, 2020 Cells interact mechanically with their surrounding by exerting forces and sensing forces or force-induced displacements. Traction force microscopy (TFM), purported to map cell-generated forces or stresses, represents an important tool that has powered ...
Yu-li Wang, Yun-Chu Lin
semanticscholar +4 more sources
Measuring (biological) materials mechanics with atomic force microscopy. 5. Traction force microscopy (cell traction forces). [PDF]
Microsc Res Tech, 2023 AbstractCells generate traction forces to probe the mechanical properties of the surroundings and maintain a basal equilibrium state of stress. Traction forces are also implicated in cell migration, adhesion and ECM remodeling, and alteration of these forces is often observed in pathologies such as cancer.
Gil-Redondo JC +3 more
europepmc +3 more sources
Rho/ROCK mechanosensor in adipocyte stiffness and traction force generation. [PDF]
Biochem Biophys Res Commun, 2022 It is increasingly recognized that interaction of adipose cells with extracellular mechanophysical milieus may play a role in regulating adipogenesis and differentiated adipocyte function and such interaction can be mediated by the mechanics of adipose ...
Bouzid T +7 more
europepmc +2 more sources
Hip Rotation and Femoral Anteversion and Its Influence on Traction Force of the Pulled Limb in Hip Arthroscopy [PDF]
Orthopaedic Journal of Sports MedicineBackground: Several variables may affect the traction force during hip arthroscopy. Specifically, the degree of hip joint rotation may influence the magnitude of traction force during hip arthroscopy.
Guanying Gao MD +4 more
doaj +2 more sources