Results 201 to 210 of about 212,052 (223)
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Traction Force Microscopy for Studying B Lymphocyte Mechanosensing.
Methods in molecular biologyB lymphocytes (B cells) are effector cells in humoral immunity. B cells recognize membrane-bound antigens through B-cell receptors in vivo, triggering signaling cascades and resulting in B-cell activation. During this process, cells exert myosin II-mediated traction to discriminate between different antigen densities and affinities.
Yue Xu +4 more
semanticscholar +3 more sources
Traction force microscopy in physics and biology
Soft Matter, 2014Adherent cells, crawling slugs, peeling paint, sessile liquid drops, bearings and many other living and non-living systems apply forces to solid substrates. Traction force microscopy (TFM) provides spatially-resolved measurements of interfacial forces through the quantification and analysis of the deformation of an elastic substrate.
Robert W, Style +8 more
openaire +2 more sources
Quantifying Immune Cell Force Generation Using Traction Force Microscopy.
Methods in molecular biology, 2023Immune cells rely on the generation of mechanical force to carry out their function. Consequently, there is a pressing need for quantitative methodologies that permit the probing of the spatio-temporal distribution of mechanical forces generated by immune cells. In this chapter, we provide a guide to quantify immune cell force generation using traction
Marcel Issler +2 more
semanticscholar +3 more sources
International Journal of Biochemistry and Cell Biology, 2023
The forces that cells, tissues, and organisms exert on the surface of a soft substrate can be measured using Traction Force Microscopy (TFM), an important and well-established technique in Mechanobiology. The usual TFM technique (two-dimensional, 2D TFM)
H. Delanoë-Ayari +4 more
semanticscholar +1 more source
The forces that cells, tissues, and organisms exert on the surface of a soft substrate can be measured using Traction Force Microscopy (TFM), an important and well-established technique in Mechanobiology. The usual TFM technique (two-dimensional, 2D TFM)
H. Delanoë-Ayari +4 more
semanticscholar +1 more source
bioRxiv
We have recently documented significant compressible behaviors in hydrogels implemented in 3D traction force microscopy (TFM). Therefore, here we have developed a new computational pipeline that accounts for this observation. Additionally, the new method
Gabriel Peery +5 more
semanticscholar +1 more source
We have recently documented significant compressible behaviors in hydrogels implemented in 3D traction force microscopy (TFM). Therefore, here we have developed a new computational pipeline that accounts for this observation. Additionally, the new method
Gabriel Peery +5 more
semanticscholar +1 more source
Measurement of Minute Cellular Forces by Traction Force Microscopy
2022The ability of living cells to exert forces on their surrounding environment, such as the extracellular matrix (ECM) or neighboring cells, plays an important role in numerous biological processes. This chapter describes a simple protocol to measure forces exerted by living cells using traction force microscopy. This approach is based on the measurement
openaire +2 more sources
Traction Force Microscopy in Differentiating Cells
Computational Methods in Applied Sciences (Springer), 2019This chapter provides an overview of the traction force microscopy method used to measure forces applied by cells on substrates under different culture conditions and particularly, as reviewed here, during differentiation. Different relevant mathematical and computational theories for traction measurement are reviewed here, and are being compared based
Amit Gefen +2 more
exaly +2 more sources
Cell Traction Force Microscopy
2007Cell traction forces are vital for many biological processes, including angiogenesis, inflammation, wound healing, and metastasis. The study of cell traction forces enables us to better understand the mechanisms of these biological processes at the cellular and molecular levels.
James H. -C. Wang +2 more
openaire +1 more source
Journal of materials chemistry. B
Over the past few decades, the critical role played by cellular contractility associated mechanotransduction in the regulation of cell functions has been revealed.
Yingjun Yang +12 more
semanticscholar +1 more source
Over the past few decades, the critical role played by cellular contractility associated mechanotransduction in the regulation of cell functions has been revealed.
Yingjun Yang +12 more
semanticscholar +1 more source
Quantitative Analysis of Myofibroblast Contraction by Traction Force Microscopy
2021Myofibroblasts play important roles in physiological processes such as wound healing and tissue repair. While high contractile forces generated by the actomyosin network enable myofibroblasts to physically contract the wound and bring together injured tissue, prolonged and elevated levels of contraction also drive the progression of fibrosis and cancer.
Yang, Shuying +3 more
openaire +2 more sources