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Roles of target site location and sequence complementarity in trans‐acting siRNA formation in Arabidopsis [PDF]

open access: closedThe Plant Journal, 2011
SummaryIn plants, many mRNAs and non‐coding RNAs are cleaved by RNA‐induced silencing complexes. After cleavage, only a limited number of RNAs are processed into trans‐acting siRNAs (tasiRNAs). One reason is that 22 nt small RNAs, but not the more common 21 nt small RNAs, can efficiently trigger tasiRNA formation.
Changqing Zhang   +3 more
openalex   +4 more sources

Molecular phylogeny of miR390-guided trans-acting siRNA genes (TAS3) in the grass family

open access: closedPlant Systematics and Evolution, 2009
Trans-acting siRNAs (tasiRNAs) are a plant-specific class of 21-nt endogenous siRNAs that function as miRNA-like posttranscriptional negative regulators. Several tasiRNA loci (known as TAS genes) have been characterized to date in rice and Arabidopsis. The TAS3 family is distinguished from other TAS loci by the dual miR390 complementary sites flanking ...
Dan Shen   +5 more
openalex   +3 more sources

The dsRNA-binding protein DRB4 interacts with the Dicer-like protein DCL4 in vivo and functions in the trans-acting siRNA pathway

open access: closedPlant Molecular Biology, 2007
Arabidopsis thaliana encodes four Dicer-like (DCL) proteins and five dsRNA-binding (DRB) proteins. We have previously demonstrated that DCL4 specifically interacts with DRB4 in vitro. Here we describe the interaction between DCL4 and DRB4 in vivo. The phenotype of a mutant with a defect in DCL4 (dcl4-2) was similar to that of a mutant with a defect in ...
Yukihiro Nakazawa   +3 more
openalex   +4 more sources

Characterisation of differentially expressed trans-acting siRNA (tasiRNAs) facing stress conditions in melon

open access: closed, 2018
[ES] Los trans-acting siRNAs (ta-siRNAs) son una clase de pequeños RNAs (20 a 24 nts) reguladores descritos en plantas. Se producen a partir de regiones especí cas del genoma llamadas genes TAS. Los transcritos derivados de estos genes TAS son reconocidos y procesados por un miRNA y por una RNA polimerasa RNA dependiente 6 (RDR6) para generar un RNA de
Cervera Seco, Luís Manuel
openalex   +2 more sources
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Trans-acting siRNAs in soybean

2016
Organizer: Center for Soybean Research of the Partner State Key Laboratory of Agrobiotechnology, CUHK ; Co-organizers: Peacock Program, Shenzhen Research Institute and New Asia College ...
openaire   +1 more source

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