Results 21 to 30 of about 46,086 (263)
A pathway for the biogenesis of trans-acting siRNAs in Arabidopsis [PDF]
Genes & Development, 2005 The Arabidopsis genes, TAS2 and TAS1a, produce structurally similar noncoding transcripts that are transformed into short (21-nucleotide [nt]) and long (24-nt) siRNAs by RNA silencing pathways. Some of these short siRNAs direct the cleavage of protein-coding transcripts, and thus function as trans-acting siRNAs (ta-siRNAs).
Manabu Yoshikawa +3 more
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Ancient trans-Acting siRNAs Confer Robustness and Sensitivity onto the Auxin Response [PDF]
Developmental Cell, 2016 ABSTRACTNovel developmental programs often evolve via cooption of existing genetic networks. To understand this process, we explored cooption of theTAS3tasiRNA pathway in the mossPhyscomitrella patens. We find an ancestral function for this repeatedly redeployed pathway in the spatial regulation of a conserved set of Auxin Response Factors.
Yevgeniy Plavskin +6 more
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The CaMV transactivator/viroplasmin interferes with RDR6-dependent trans-acting and secondary siRNA pathways in Arabidopsis [PDF]
Nucleic Acids Research, 2008 Several RNA silencing pathways in plants restrict viral infections and are suppressed by distinct viral proteins. Here we show that the endogenous trans-acting (ta)siRNA pathway, which depends on Dicer-like (DCL) 4 and RNA-dependent RNA polymerase (RDR) 6, is suppressed by infection of Arabidopsis with Cauliflower mosaic virus (CaMV).
P. V. Shivaprasad +6 more
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Specification of Leaf Polarity in Arabidopsis via the trans-Acting siRNA Pathway [PDF]
Current Biology, 2006 Plants leaves develop proximodistal, dorsoventral (adaxial-abaxial), and mediolateral patterns following initiation. The Myb domain gene PHANTASTICA (PHAN) is required for adaxial fate in many plants , but the Arabidopsis ortholog ASYMMETRIC LEAVES1 (AS1) has milder effects, suggesting that alternate or redundant pathways exist .
Damien Garcia +3 more
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MicroRNAs as master regulators of the plant NB-LRR defense gene family via the production of phased, trans-acting siRNAs [PDF]
Genes & Development, 2011 Legumes and many nonleguminous plants enter symbiotic interactions with microbes, and it is poorly understood how host plants respond to promote beneficial, symbiotic microbial interactions while suppressing those that are deleterious or pathogenic. Trans-acting siRNAs (tasiRNAs) negatively regulate target transcripts and are characterized by siRNAs ...
Jixian Zhai +15 more
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Trans-acting siRNA-mediated repression of ETTIN and ARF4 regulates heteroblasty inArabidopsis [PDF]
Development, 2006 Mutations in the ARGONAUTE gene ZIPPY(ZIP)/AGO7 in Arabidopsis accelerate the juvenile-to-adult transition. A screen for mutations that suppress this precocious phenotype yielded alleles of two auxin-related transcription factors known to be upregulated in zip: ETTIN (ETT)/ARF3 and ARF4.
Christine Hunter +5 more
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The Plant Journal, 2021 SUMMARYTargeted gene silencing using small regulatory RNAs is a widely used technique for genetic studies in plants. Artificial microRNAs are one common approach, as they have the advantage of producing just a single functional small RNA, which can be designed for high target specificity and low off‐target effects.
Alice Lunardon +2 more
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SDE5, the putative homologue of a human mRNA export factor, is required for transgene silencing and accumulation of trans‐acting endogenous siRNA [PDF]
The Plant Journal, 2007 SummaryPost‐transcriptional gene silencing (PTGS) is a sequence‐specific RNA degradation process conserved in fungi, plants and animals. The trigger of the mechanism is double‐stranded RNA derived from transgenic or endogenous loci and formed by intra‐ or inter‐molecular interactions of single‐stranded RNAs or the action of RNA‐dependent RNA ...
Inmaculada Hernández‐Pinzón +5 more
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Assorted Processing of Synthetic Trans-Acting siRNAs and Its Activity in Antiviral Resistance
PLOS ONE, 2015 The use of syn-tasiRNAs has been proposed as an RNA interference technique alternative to those previously described: hairpin based, virus induced gene silencing or artificial miRNAs. In this study we engineered the TAS1c locus to impair Plum pox virus (PPV) infection by replacing the five native siRNAs with two 210-bp fragments from the CP and the 3 ...
Mingmin Zhao +4 more
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Identification of novel drought-responsive microRNAs and trans-acting siRNAs from Sorghum bicolor (L.) Moench by high-throughput sequencing analysis [PDF]
Frontiers in Plant Science, 2015 Small non-coding RNAs (sRNAs) namely microRNAs (miRNAs) and trans-acting small interfering RNAs (tasi-RNAs) play a crucial role in post-transcriptional regulation of gene expression and thus the control plant development and stress responses. In order to identify drought-responsive miRNAs and tasi-RNAs in sorghum, we constructed small RNA libraries ...
Amit Katiyar +5 more
openalex +5 more sources