Results 61 to 70 of about 2,895,452 (235)
Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells
FEBS Open Bio, EarlyView.Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley +1 more source
A PLASTIC MEDIUM: TRANS-HUMAN VISUAL SPECULATIVE FICTION AND TRANS MEN’S GAZE
, 2022 342 pages“A Plastic Medium” considers a trans masculine prosthetic, colloquially called a packer, as both a cultural product and a media object. A packer provides not only a modified way of being in the world, but a way of looking at the world, and is ...
Cole, Joshua
core +1 more source
Characterization of the key membrane protein cytochrome Cyc2 from Ghiorsea bivora
FEBS Open Bio, EarlyView.This study reports the first detailed biochemical analysis of Cyc2TAG1_1, a cytochrome protein involved in microbial iron oxidation. We produced and purified this enzyme in E. coli, then measured its iron‐oxidizing efficiency, and characterized its electrical and structural properties.
Josiane Azizi +6 more
wiley +1 more source
Cell Regeneration, 2015 Background: Currently, direct conversion from somatic cells to neurons requires virus-mediated delivery of at least one transcriptional factor or a combination of several small-molecule compounds.
Songwei He +20 more
doaj +1 more source
Человек и его здоровье, 2023 Low-quality herbal raw materials and the products obtained from them are important sources of various ecotoxicants, especially heavy metals, entering the human body. Aqueous extractions of medicinal plant raw materials - decoctions and infusions - are of a particular interest in this respect - as they are the most accessible and frequently used dosage ...
openaire +1 more source
, 2015 The subdirectory /MD includes five free MD simulation trajectories of GluK2-trans complex described in the manuscript. The subdirectory /isomerization includes five forced switching simulation trajectories from trans-gluazo to cis-gluazo.
Marcus Elstner (394014) +11 more
core +1 more source
FEBS Open Bio, EarlyView.A rapid visual test combines DNA amplification, CRISPR–based target verification, and a lateral flow strip to detect Streptococcus suis. The assay gives results in about 45 min, reduces false‐positive interpretation, and does not require fluorescence equipment.
Liu‐xin Li +7 more
wiley +1 more source
Discovery of the Elusive Carbonic Acid (HOCOOH) in Space
The Astrophysical Journal, 2023 A quarter century after the detection of the last interstellar carboxylic acid, acetic acid (CH _3 COOH), we report the discovery of a new one, the cis-trans form of carbonic acid (HOCOOH), toward the Galactic center molecular cloud G+0.693–0.027. HOCOOH
Miguel Sanz-Novo +14 more
doaj +1 more source
RNA Sequencing Resolves Cryptic Pathogenic Variants in Mitochondrial Disease
Annals of Clinical and Translational Neurology, EarlyView.ABSTRACT Objective Mitochondrial diseases are the most common inherited metabolic disorders, characterized by pronounced clinical and genetic heterogeneity that complicates molecular diagnosis. Although DNA‐based sequencing approaches have become standard in genetic testing, up to half of patients remain without a definitive diagnosis.
Zhimei Liu +21 more
wiley +1 more source
A tethered bilayer assembled on top of immobilized calmodulin to mimic cellular compartmentalization. [PDF]
PLoS ONE, 2011 BackgroundBiomimetic membrane models tethered on solid supports are important tools for membrane protein biochemistry and biotechnology. The supported membrane systems described up to now are composed of a lipid bilayer tethered or not to a surface ...
Claire Rossi +6 more
doaj +1 more source