Results 31 to 40 of about 426,916 (271)

Gene-centric coverage of the human liver transcriptome: QPCR, Illumina, and Oxford Nanopore RNA-Seq

Frontiers in Molecular Biosciences, 2022
It has been shown that the best coverage of the HepG2 cell line transcriptome encoded by genes of a single chromosome, chromosome 18, is achieved by a combination of two sequencing platforms, Illumina RNA-Seq and Oxford Nanopore Technologies (ONT), using
Ekaterina V. Ilgisonis, Elena A. Ponomarenko, Svetlana N. Tarbeeva, Andrey V. Lisitsa, Victor G. Zgoda, Sergey P. Radko, Alexander I. Archakov   +6 more
doaj   +1 more source

A histidine‐rich extension of the mitochondrial F0 subunit ATP6 from the ice worm Mesenchytraeus solifugus increases ATP synthase activity in bacteria

FEBS Letters, EarlyView.
The glacier ice worm Mesenchytraeus solifugus survives year‐round at 0 °C. Its ATP6 subunit, which forms a regulatory component of the proton pore in mitochondrial ATP synthase, has a carboxy‐terminal extension not found in any other organism examined to date. Here, we show that fusion of this extension to the homologous AtpB protein in E. coli results
Truman Dunkley, Daniel H. Shain, Eric A. Klein   +2 more
wiley   +1 more source

Intracellular Staphylococcus aureus persisters upon antibiotic exposure

Nature Communications, 2020
Bacterial persister cells exhibit a transient non-growing state and antibiotic tolerance. Here, Peyrusson et al. provide evidence of metabolically active Staphylococcus aureus persisters within infected host cells exposed to antibiotics and analyse ...
Frédéric Peyrusson, Hugo Varet, Tiep Khac Nguyen, Rachel Legendre, Odile Sismeiro, Jean-Yves Coppée, Christiane Wolz, Tanel Tenson, Françoise Van Bambeke   +8 more
doaj   +1 more source

Characteristics of the Kelch domain containing (KLHDC) subfamily and relationships with diseases

FEBS Letters, EarlyView.
The Kelch protein superfamily includes 63 members, with the KLHDC subfamily having 10 proteins. While their functions are not fully understood, recent advances in KLHDC2's structure and role in protein degradation have highlighted its potential for drug development, especially in PROTAC therapies.
Courtney Pilcher, Paula Armina V. Buco, Jia Q. Truong, Paul A. Ramsland, Monique F. Smeets, Carl R. Walkley, Jessica K. Holien   +6 more
wiley   +1 more source

Unconventional mRNA processing and degradation pathways for the polycistronic yrzI (spyTA) mRNA in Bacillus subtilis

FEBS Letters, EarlyView.
The S1025 peptide is the major antidote to the YrzI toxin, which we renamed here as SpyT (Small Peptide YrzI Toxin) and SpyA (Small Peptide YrzI Antitoxin) (1). Degradation of the toxin–antitoxin spyTA mRNA, either by a translation‐dependent cleavage by the endoribonuclease Rae1 (2) or by direct attack by 3′‐exoribonucleases (3), also contributes to ...
Laetitia Gilet, Magali Leroy, Alexandre Maes, Ciarán Condon, Frédérique Braun   +4 more
wiley   +1 more source

Decryption of sequence, structure, and functional features of SINE repeat elements in SINEUP non-coding RNA-mediated post-transcriptional gene regulation

Nature Communications
RNA structure folding largely influences RNA regulation by providing flexibility and functional diversity. In silico and in vitro analyses are limited in their ability to capture the intricate relationships between dynamic RNA structure and RNA ...
Harshita Sharma, Matthew N. Z. Valentine, Naoko Toki, Hiromi Nishiyori Sueki, Stefano Gustincich, Hazuki Takahashi, Piero Carninci   +6 more
doaj   +1 more source

Imputed genomes of historical horses provide insights into modern breeding

iScience, 2023
Summary: Historical genomes can provide important insights into recent genomic changes in horses, especially the development of modern breeds. In this study, we characterized 8.7 million genomic variants from a panel of 430 horses from 73 breeds ...
Evelyn T. Todd, Aurore Fromentier, Richard Sutcliffe, Yvette Running Horse Collin, Aude Perdereau, Jean-Marc Aury, Camille Èche, Olivier Bouchez, Cécile Donnadieu, Patrick Wincker, Ted Kalbfleisch, Jessica L. Petersen, Ludovic Orlando   +12 more
doaj  

The topology of the transcription regulatory network in the yeast, S. cerevisiae [PDF]

Physica A, vol. 318/3-4 (2003) pp. 601-612, 2002
MOTIVATION: A central goal of postgenomic biology is the elucidation of the regulatory relationships among all cellular constituents that together comprise the 'genetic network' of a cell or microorganism. Experimental manipulation of gene activity coupled with the assessment of perturbed transcriptome (i.
arxiv   +1 more source

Social context prevents heat hormetic effects against mutagens during fish development

FEBS Letters, EarlyView.
This study shows that sublethal heat stress protects fish embryos against ultraviolet radiation, a concept known as ‘hormesis’. However, chemical stress transmission between fish embryos negates this protective effect. By providing evidence for the mechanistic molecular basis of heat stress hormesis and interindividual stress communication, this study ...
Lauric Feugere, Claudio Silva De Freitas, Adam Bates, Kenneth B. Storey, Pedro Beltran‐Alvarez, Katharina C. Wollenberg Valero   +5 more
wiley   +1 more source

Small RNA Deep-Sequencing Analyses Reveal a New Regulator of Virulence in Agrobacterium fabrum C58

Molecular Plant-Microbe Interactions, 2015
Novel ways of regulating Ti plasmid functions were investigated by studying small RNAs (sRNAs) that are known to act as posttranscriptional regulators in plant pathogenic bacteria.
M. Dequivre, B. Diel, C. Villard, O. Sismeiro, M. Durot, J. Y. Coppée, X. Nesme, L. Vial, F. Hommais   +8 more
doaj   +1 more source