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Group A Streptococcus Transcriptome Analysis

2020
Global gene expression analyses in bacteria have undergone a dramatic transformation. Prior to the development of high-throughput sequencing technologies, real-time PCR or microarray studies were the mainstay of assessing differences in gene expression in bacteria. Real-time PCR remains a critical tool for targeted gene expression analyses.
Misú, Sanson, Anthony R, Flores
openaire   +2 more sources

Novel algorithm for transcriptome analysis

Physiological Genomics, 2006
A growing body of evidence implicates the oocyte as a key regulator of ovarian folliculogenesis and early embryonic development. We have screened bovine cDNA microarrays (containing expressed sequence tags representing >15,000 unique genes) with Cy3- and Cy5-labeled cDNA derived from bovine oocyte samples collected at two different stages of ...
Peter M, Saama   +4 more
openaire   +2 more sources

Analysis of the Plant Mitochondrial Transcriptome

2021
Transcriptome analyses are widely performed, but the analysis of organelle genome expression is often overlooked. In this chapter, we describe three methods to analyse the accumulation, splicing, editing and processing of plant mitochondrial transcript expression: a classical RT-qPCR assay, an RNA-Seq approach with its bioinformatical and statistical ...
Baudry, Kévin   +2 more
openaire   +3 more sources

Transcriptomic Analysis of Trypanosomatids

The study of transcriptomics in trypanosomatids is essential for understanding their unique gene expression mechanisms, which are predominantly regulated post-transcriptionally due to polycistronic transcription and a lack of specific promoters. These parasites, responsible for diseases such as Chagas disease and leishmaniasis, pose significant global ...
Carlos Mario, Ospina   +3 more
openaire   +2 more sources

Analysis of the wheat endosperm transcriptome

Journal of Applied Genetics, 2006
Among the cereals, wheat is the most widely grown geographically and is part of the staple diet in much of the world. Understanding how the cereal endosperm develops and functions will help generate better tools to manipulate grain qualities important to end-users. We used a genomics approach to identify and characterize genes that are expressed in the
Debbie L, Laudencia-Chingcuanco   +4 more
openaire   +2 more sources

Transcriptome Analysis

Transcriptome analysis reveals the relationship between Spring viremia of carp N protein induced Endoplasmic reticulum stress in EPC cells and apoptosis and ...
Dinesh Velayutham   +4 more
openaire   +2 more sources

Transcriptome analysis of equine sarcoids

Veterinary and Comparative Oncology, 2016
AbstractEquine sarcoids are the most commonly detected skin tumours in Equidae. In the present research, a comparative transcriptomic analysis was performed which aimed at looking inside a tumour biology and identification of the expression profile as a potential source of cancer specific genes useful as biomarkers.
Semik E   +6 more
openaire   +4 more sources

Transcriptome analysis in heart failure

Current Opinion in Cardiology, 2016
Genome-wide analysis of RNA abundances (transcriptome analysis) offers great potential to identify biomarkers of disease and to gain insight into mechanisms underlying the development of heart failure. I will discuss key factors in generating and evaluating transcriptome data, and recent studies that have contributed to the understanding of cardiac ...
openaire   +2 more sources

Generation and Analysis of Transcriptomics Data

2010
Transcript profiling ("Transcriptomics") is a widely used technique that obtains information on the abundance of multiple mRNA transcripts within a biological sample simultaneously. Therefore, when a number of such samples are analysed, as in a scientific experiment, large and complex data sets are gene-rated.
Philip D, Glaves, Jonathan D, Tugwood
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Large-Scale Transcriptome Analysis

2017
Genomic variants identified to be linked with complex traits such as blood pressure are fewer in coding sequences compared to noncoding sequences. This being the case, there is an increasing need to query the expression of genes at a genome scale to then correlate the cause of alteration in expression to the function of variants regardless of where ...
David, Weaver   +2 more
openaire   +2 more sources

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