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RNA Extraction for Transcriptome Analysis

2021
RNA is a representation of the expressed genes of an organism. RNA-Seq based transcriptome analysis has been instrumental in quantifying gene expression or the sequence information of full-length transcripts including alternate spliced-isoforms. A suitable RNA-extraction method is the crucial first step for all projects using transcriptome analysis to ...
Furtado, Agnelo, Henry, Robert J.
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Group A Streptococcus Transcriptome Analysis

2020
Global gene expression analyses in bacteria have undergone a dramatic transformation. Prior to the development of high-throughput sequencing technologies, real-time PCR or microarray studies were the mainstay of assessing differences in gene expression in bacteria. Real-time PCR remains a critical tool for targeted gene expression analyses.
Misú, Sanson, Anthony R, Flores
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Novel algorithm for transcriptome analysis

Physiological Genomics, 2006
A growing body of evidence implicates the oocyte as a key regulator of ovarian folliculogenesis and early embryonic development. We have screened bovine cDNA microarrays (containing expressed sequence tags representing >15,000 unique genes) with Cy3- and Cy5-labeled cDNA derived from bovine oocyte samples collected at two different stages of ...
Peter M, Saama   +4 more
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Analysis of the Plant Mitochondrial Transcriptome

2021
Transcriptome analyses are widely performed, but the analysis of organelle genome expression is often overlooked. In this chapter, we describe three methods to analyse the accumulation, splicing, editing and processing of plant mitochondrial transcript expression: a classical RT-qPCR assay, an RNA-Seq approach with its bioinformatical and statistical ...
Baudry, Kévin   +2 more
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Transcriptomic Analysis of Trypanosomatids

The study of transcriptomics in trypanosomatids is essential for understanding their unique gene expression mechanisms, which are predominantly regulated post-transcriptionally due to polycistronic transcription and a lack of specific promoters. These parasites, responsible for diseases such as Chagas disease and leishmaniasis, pose significant global ...
Carlos Mario, Ospina   +3 more
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Analysis of the wheat endosperm transcriptome

Journal of Applied Genetics, 2006
Among the cereals, wheat is the most widely grown geographically and is part of the staple diet in much of the world. Understanding how the cereal endosperm develops and functions will help generate better tools to manipulate grain qualities important to end-users. We used a genomics approach to identify and characterize genes that are expressed in the
Debbie L, Laudencia-Chingcuanco   +4 more
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Transcriptome Analysis

Transcriptome analysis reveals the relationship between Spring viremia of carp N protein induced Endoplasmic reticulum stress in EPC cells and apoptosis and ...
Dinesh Velayutham   +4 more
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Transcriptome analysis of equine sarcoids

Veterinary and Comparative Oncology, 2016
AbstractEquine sarcoids are the most commonly detected skin tumours in Equidae. In the present research, a comparative transcriptomic analysis was performed which aimed at looking inside a tumour biology and identification of the expression profile as a potential source of cancer specific genes useful as biomarkers.
Semik E   +6 more
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Transcriptome analysis in heart failure

Current Opinion in Cardiology, 2016
Genome-wide analysis of RNA abundances (transcriptome analysis) offers great potential to identify biomarkers of disease and to gain insight into mechanisms underlying the development of heart failure. I will discuss key factors in generating and evaluating transcriptome data, and recent studies that have contributed to the understanding of cardiac ...
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