Results 151 to 160 of about 320,395 (368)

Microanatomy of the Human Atherosclerotic Plaque by Single-Cell Transcriptomics

Circulation Research, 2020
Supplemental Digital Content is available in the text. Rationale: Atherosclerotic lesions are known for their cellular heterogeneity, yet the molecular complexity within the cells of human plaques has not been fully assessed. Objective: Using single-cell
M. Depuydt, K. Prange, L. Slenders, T. Örd, D. Elbersen, A. Boltjes, S. D. de Jager, F. Asselbergs, G. D. de Borst, E. Aavik, T. Lönnberg, E. Lutgens, C. Glass, H. D. den Ruijter, M. Kaikkonen, I. Bot, B. Slütter, S. W. van der Laan, S. Yla-Herttuala, M. Mokry, J. Kuiper, M. D. de Winther, G. Pasterkamp   +22 more
semanticscholar   +1 more source

RNA-Seq Mapping Errors When Using Incomplete Reference Transcriptomes of Vertebrates [PDF]

arXiv, 2013
Whole transcriptome sequencing is increasingly being used as a functional genomics tool to study non- model organisms. However, when the reference transcriptome used to calculate differential expression is incomplete, significant error in the inferred expression levels can result. In this study, we use simulated reads generated from real transcriptomes
arxiv  

Evidence of neutral transcriptome evolution in plants [PDF]

, 2008
The transcriptome of an organism is its set of gene transcripts (mRNAs) at a defined spatial and temporal locus. Because gene expression is affected markedly by environmental and developmental perturbations, it is widely assumed that transcriptome ...
Bailey, Beilstein, Brady, Broadley, Ceplitis, Craigon, Felsenstein, Filatov, Gilad, Gould, Graham, H. C. Bowen, Hammond, Hammond, Hampton, Hawes, J. P. Hammond, J. W. McNicol, Johnston, Khaitovich, Khaitovich, Khaitovich, Kimura, Koch, M. R. Broadley, N. S. Graham, Narsai, P. J. White, P. P. M. Iannetta, R. G. Fray, S. T. May, Schmid, Schranz, Soltis, Z. F. Emmerson   +34 more
core   +1 more source

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

Molecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, James S. Fraser, Martin Figeac, Alexis B. Cortot, Etienne Rouleau, David Tulasne   +14 more
wiley   +1 more source

Identification of a rare SEPT9 variant in a family with autosomal dominant Charcot-Marie-Tooth disease

BMC Medical Genetics, 2020
Background Charcot-Marie-Tooth disease (CMT) is one of the most commonly inherited neurological disorders. A growing number of genes, involved in glial and neuronal functions, have been associated with different subtypes of CMT leading to improved ...
Gerrit M. Grosse, Christine Bauer, Bruno Kopp, Christoph Schrader, Alma Osmanovic   +4 more
doaj   +1 more source

A joint model of unpaired data from scRNA-seq and spatial transcriptomics for imputing missing gene expression measurements [PDF]

arXiv, 2019
Spatial studies of transcriptome provide biologists with gene expression maps of heterogeneous and complex tissues. However, most experimental protocols for spatial transcriptomics suffer from the need to select beforehand a small fraction of genes to be quantified over the entire transcriptome.
arxiv  

Response to neoadjuvant chemotherapy in early breast cancers is associated with epithelial–mesenchymal transition and tumor‐infiltrating lymphocytes

Molecular Oncology, EarlyView.
Epithelial–mesenchymal transition (EMT) and tumor‐infiltrating lymphocytes (TILs) are associated with early breast cancer response to neoadjuvant chemotherapy (NAC). This study evaluated EMT and TIL shifts, with immunofluorescence and RNA sequencing, at diagnosis and in residual tumors as potential biomarkers associated with treatment response.
Françoise Derouane, Jérôme Ambroise, Cédric van Marcke, Mieke Van Bockstal, Martine Berlière, Christine Galant, Hélène Dano, Médina Lougué, Elena Benidovskaya, Guy Jerusalem, Vincent Bours, Claire Josse, Jérôme Thiry, Aurélie Daumerie, Caroline Bouzin, Cyril Corbet, François P. Duhoux   +16 more
wiley   +1 more source

A rapid and simple clonality assay for bovine leukemia virus-infected cells by amplified fragment length polymorphism (AFLP) analysis

Microbiology Spectrum
Enzootic bovine leukosis (EBL), although eradicated in some European countries, is still the most common neoplastic disease of cattle, caused by the bovine leukemia virus (BLV).
Tomoko Kobayashi, Sakurako Makimoto, Nagaki Ohnuki, Md Belal Hossain, M. Ishrat Jahan, Misaki Matsuo, Kazuhiko Imakawa, Yorifumi Satou   +7 more
doaj   +1 more source

Molecular and functional profiling unravels targetable vulnerabilities in colorectal cancer

Molecular Oncology, EarlyView.
We used whole exome and RNA‐sequencing to profile divergent genomic and transcriptomic landscapes of microsatellite stable (MSS) and microsatellite instable (MSI) colorectal cancer. Alterations were classified using a computational score for integrative cancer variant annotation and prioritization.
Efstathios‐Iason Vlachavas, Konstantinos Voutetakis, Vivian Kosmidou, Spyridon Tsikalakis, Spyridon Roditis, Konstantinos Pateas, Ryangguk Kim, Kymberleigh Pagel, Stephan Wolf, Gregor Warsow, Antonia Dimitrakopoulou‐Strauss, Georgios N. Zografos, Alexander Pintzas, Johannes Betge, Olga Papadodima, Stefan Wiemann   +15 more
wiley   +1 more source

The plasticity of the mammalian transcriptome

Genomics, 2010
The dogmatic view of RNA as a mere necessity in the transfer of information between DNA and proteins has during recent years come into question. Novel approaches and new technology has revealed an unprecedented level of inherent complexity in the mammalian transcriptome.
Johan Lindberg, Joakim Lundeberg
openaire   +3 more sources