Results 101 to 110 of about 411,981 (355)

The Axonal Membrane Protein PRG2 Inhibits PTEN and Directs Growth to Branches

Cell Reports, 2019
Summary: In developing neurons, phosphoinositide 3-kinases (PI3Ks) control axon growth and branching by positively regulating PI3K/PI(3,4,5)P3, but how neurons are able to generate sufficient PI(3,4,5)P3 in the presence of high levels of the antagonizing
Annika Brosig, Joachim Fuchs, Fatih Ipek, Cristina Kroon, Sandra Schrötter, Mayur Vadhvani, Alexandra Polyzou, Julia Ledderose, Michiel van Diepen, Hermann-Georg Holzhütter, Thorsten Trimbuch, Niclas Gimber, Jan Schmoranzer, Ivo Lieberam, Christian Rosenmund, Christian Spahn, Patrick Scheerer, Michal Szczepek, George Leondaritis, Britta J. Eickholt   +19 more
doaj   +1 more source

A quantitative comparison of sRNA-based and protein-based gene regulation [PDF]

, 2008
Small, non-coding RNAs (sRNAs) play important roles as genetic regulators in prokaryotes. sRNAs act post-transcriptionally via complementary pairing with target mRNAs to regulate protein expression.
Berg HC, Ned S Wingreen, Pankaj Mehta, Ptashne M, Sidhartha Goyal, van Kampen NG   +5 more
core   +3 more sources

A 3D Astrocyte Microenvironment Model Enables Rapid Ca2+‐Resolved Analysis and Therapeutic Modulation of Oxidative Neuroinflammation

Advanced Healthcare Materials, EarlyView.
This work presents ARC‐3D, a soft 3D model that recreates how brain support cells, called astrocytes, react to oxidative stress. The system visualizes rapid calcium changes and inflammatory signals, and shows how the drug KDS12025 can protect cells from damage. ARC‐3D offers a simple, reliable way to study early drivers of brain inflammation.
Ju‐Kang Kim, Ye‐Ji Kim, Seyoung Ryu, Daeui Park, Kyoung‐Sik Moon, Yu Bin Lee, Dong Ho Woo   +6 more
wiley   +1 more source

Protocol to investigate G protein-coupled receptor signaling kinetics and concentration-dependent responses using ONE-GO biosensors

STAR Protocols
Summary: ONE vector G protein optical (ONE-GO) biosensors are versatile tools to measure the activity of G protein-coupled receptors (GPCRs) in cells. The availability of ONE-GO biosensors for ten active Gα subunits representative of all four G protein ...
Remi Janicot, Mikel Garcia-Marcos
doaj   +1 more source

AIM for Allostery: Using the Ising Model to Understand Information Processing and Transmission in Allosteric Biomolecular Systems

Entropy, 2015
In performing their biological functions, molecular machines must process and transmit information with high fidelity. Information transmission requires dynamic coupling between the conformations of discrete structural components within the protein ...
Michael V. LeVine, Harel Weinstein
doaj   +1 more source

Nonlinear excitations match correlated motions unveiled by NMR in proteins: a new perspective on allosteric cross-talk

, 2014
In this paper we propose a novel theoretical framework for interpreting long-range dynamical correlations unveiled in proteins through NMR measurements.
Piazza, Francesco
core   +2 more sources

Direct Integration of Micromachined Pipettes in a Flow Channel for Single DNA Molecule Study by Optical Tweezers [PDF]

, 2001
We have developed a micromachined flow cell consisting of a flow channel integrated with micropipettes. The flow cell is used in combination with an optical trap setup (optical tweezers) to study mechanical and structural properties of λ-DNA molecules ...
Bennink, Martin L., Berenschot, J.W., Berg, Albert van den, Boer, Meint J. de, Brugger, Jürgen, Elwenspoek, Miko, Greve, Jan, Grooth, Bart G. de, Jansen, Henri V., Kanger, Johannes S., Oever, Ronny van 't, Rusu, Cristina   +11 more
core   +2 more sources

A New 3D Colon on a Chip to Decipher the Influence of Mechanical Forces on the Physiological Cellular Ecosystem

Advanced Healthcare Materials, EarlyView.
To dissect how mechanical forces influence intestinal physiology, we developed a stretchable 3D colon‐on‐chip that integrates tunable topography, stiffness and peristalsis‐like motion within a physiologically relevant microenvironment. We showed that stretching is a dominant factor governing epithelial behavior, markedly enhancing proliferation and ...
Moencopi Bernheim‐Dennery, Lauriane Gérémie, Julie Brun, Lucas Chassatte, Giacomo Gropplero, Réda Bouras, Jieun Choo, Bertrand Cinquin, Alba Marcellan, Danijela Matic Vignjevic, Stéphanie Descroix   +10 more
wiley   +1 more source

A thermodynamic framework for modelling membrane transporters

, 2018
Membrane transporters contribute to the regulation of the internal environment of cells by translocating substrates across cell membranes. Like all physical systems, the behaviour of membrane transporters is constrained by the laws of thermodynamics ...
Crampin, Edmund J., Cursons, Joseph, Gawthrop, Peter J., Pan, Michael, Tran, Kenneth   +4 more
core   +1 more source

Kelvin Probe Force Microscopy in Bionanotechnology: Current Advances and Future Perspectives

Advanced Materials, EarlyView.
Kelvin probe force microscopy (KPFM) enables the nanoscale mapping of electrostatic surface potentials. While widely applied in materials science, its use in biological systems remains emerging. This review presents recent advances in KPFM applied to biological samples and provides a critical perspective on current limitations and future directions for
Ehsan Rahimi, Mario Palacios‐Corella, Arjan Mol, Salvador Pané, Josep Puigmartí‐Luis   +4 more
wiley   +1 more source