Results 21 to 30 of about 34,099 (218)

Voltage-clamp fluorometry to record flow-activated PIEZO1 currents and fluorometric signals

STAR Protocols
Summary: PIEZO channels sense mechanical forces through conformational rearrangements of a mechanosensory domain called blade. To probe these rearrangements in real time, we have inserted conformational-sensitive cyclic-permuted GFP into several ...
Tharaka Wijerathne, Jerome Lacroix
doaj   +1 more source

Using mass spectrometry imaging to visualize age-related subcellular disruption

Frontiers in Molecular Biosciences, 2023
Metabolic homeostasis balances the production and consumption of energetic molecules to maintain active, healthy cells. Cellular stress, which disrupts metabolism and leads to the loss of cellular homeostasis, is important in age-related diseases.
Kelly A. Hogan, Kelly A. Hogan, Kelly A. Hogan, Julianna D. Zeidler, Heather K. Beasley, Abrar I. Alsaadi, Abdulkareem A. Alshaheeb, Yi-Chin Chang, Hua Tian, Hua Tian, Antentor O. Hinton, Melanie R. McReynolds, Melanie R. McReynolds   +12 more
doaj   +1 more source

Cell surface interactome analysis identifies TSPAN4 as a negative regulator of PD‐L1 in melanoma

Molecular Oncology, EarlyView.
Using cell surface proximity biotinylation, we identified tetraspanin TSPAN4 within the PD‐L1 interactome of melanoma cells. TSPAN4 negatively regulates PD‐L1 expression and lateral mobility by limiting its interaction with CMTM6 and promoting PD‐L1 degradation.
Guus A. Franken, Andrea Abel Gutierrez, Imke van Rossum, Cornelia G. Spruijt, Michiel Vermeulen, Guido van Mierlo, Blanca Scheijen, Annemiek B. van Spriel   +7 more
wiley   +1 more source

NKCC1: A key regulator of glioblastoma progression

Molecular Oncology, EarlyView.
Glioblastoma (GBM) progression is driven by disrupted chloride cotransporter homeostasis. NKCC1 is highly expressed in stem‐like, astrocytic, and progenitor cells, correlating with earlier recurrence, while overall survival remains unaffected. NKCC1 serves as a prognostic marker and potential therapeutic target, linking chloride transporter imbalance ...
Anja Thomsen, Diana Freitag, Madlen Haase, Christian Senft, Falko Schwarz, Silke Keiner   +5 more
wiley   +1 more source

Altered Cell Mechanics from the Inside: Dispersed Single Wall Carbon Nanotubes Integrate with and Restructure Actin

Journal of Functional Biomaterials, 2012
With a range of desirable mechanical and optical properties, single wall carbon nanotubes (SWCNTs) are a promising material for nanobiotechnologies. SWCNTs also have potential as biomaterials for modulation of cellular structures.
Mohammad F. Islam, Kris Noel Dahl, Gustavo K. Rohde, Mohammad R. K. Mofrad, Yu-Li Wang, Brian D. Holt, Hengameh Shams, Travis A. Horst, Saurav Basu, Andrew D. Rape   +9 more
doaj   +1 more source

Evaluation of Pharmacological Rescue of Melanocortin-4 Receptor Nonsense Mutations by Aminoglycoside

Life, 2022
The melanocortin-4 receptor (MC4R) is critical for central satiety regulation, therefore presenting a potent target for pharmacological obesity treatment. Melanocortin-4 receptor mutations prevalently cause monogenetic obesity.
Friederike Höpfner, Sarah Paisdzior, Nanina Reininghaus, Iqra Sohail, Patrick Scheerer, Paolo Annibale, Heike Biebermann, Peter Kühnen   +7 more
doaj   +1 more source

A Vascular Microphysiological Model of Lung Fibrosis Reveals That Myofibroblasts and IPF Patient‐Derived Fibroblasts Impair Angiogenesis and Vasculogenesis

Advanced Functional Materials, EarlyView.
A microphysiological lung fibrosis model recapitulates myofibroblast–vascular interactions. Induced myofibroblasts and patient‐derived IPF fibroblasts impair angiogenesis and increase vascular permeability via TGF‐β1–driven signaling. Pharmacological interventions with SB 431542 and VEGF supplementation restore vascular morphology and barrier function.
Elena Cambria, Adriana Blazeski, Eunkyung Clare Ko, Tran Thai, Shania Dantes, David A. Barbie, Sarah E. Shelton, Roger D. Kamm   +7 more
wiley   +1 more source

Aluminum-Induced Entropy in Biological Systems: Implications for Neurological Disease

Journal of Toxicology, 2014
Over the last 200 years, mining, smelting, and refining of aluminum (Al) in various forms have increasingly exposed living species to this naturally abundant metal.
Christopher A. Shaw, Stephanie Seneff, Stephen D. Kette, Lucija Tomljenovic, John W. Oller, Robert M. Davidson   +5 more
doaj   +1 more source

MicroRNA-100-5p and microRNA-298-5p released from apoptotic cortical neurons are endogenous Toll-like receptor 7/8 ligands that contribute to neurodegeneration

Molecular Neurodegeneration, 2021
Background MicroRNA (miRNA) expression in the brain is altered in neurodegenerative diseases. Recent studies demonstrated that selected miRNAs conventionally regulating gene expression at the post-transcriptional level can act extracellularly as ...
Thomas Wallach, Zoé J. Mossmann, Michal Szczepek, Max Wetzel, Rui Machado, Martin Raden, Milad Miladi, Gunnar Kleinau, Christina Krüger, Paul Dembny, Drew Adler, Yuanyuan Zhai, Victor Kumbol, Omar Dzaye, Jutta Schüler, Matthias Futschik, Rolf Backofen, Patrick Scheerer, Seija Lehnardt   +18 more
doaj   +1 more source

An All‐Optical Driven Bio‐Photovoltaic Interface for Active Control of Live Cells

Advanced Functional Materials, EarlyView.
Bio‐photovoltaic Interface (BIO‐PV‐I) for live cell manipulation is presented. BIO‐PV‐I can be activated non‐invasively and remotely to control the spatial motility, adhesion, and morphology of cells adhering to it. BIO‐PV‐I uses a patterned light‐induced electric potential in iron‐doped lithium niobate crystals whose light‐driven and reversible nature,
Lisa Miccio, Martina Mugnano, Valentina Marchesano, Alejandro Calabuig, Zhe Wang, Gennaro Giordano, Francesca Borrelli, Simonetta Grilli, Pietro Ferraro   +8 more
wiley   +1 more source