Results 91 to 100 of about 932,327 (388)

Brucella NyxA and NyxB dimerization enhances effector function during infection

FEBS Letters, EarlyView.
Brucella abortus thrives inside cells thanks to the translocation of effector proteins that fine‐tune cellular functions. NyxA and NyxB are two effectors that destabilize the nucleolar localization of their host target, SENP3. We show that the Nyx proteins directly interact with each other and that their dimerization is essential for their function ...
Lison Cancade‐Veyre, Arthur Louche, Francine C. A. Gérard, Laurent Terradot, Suzana P. Salcedo   +4 more
wiley   +1 more source

Graphene‐Based MicroRNA Transfection Blocks Preosteoclast Fusion to Increase Bone Formation and Vascularization

Advancement of science, 2017
The objective of this study is to design a graphene‐based miRNA transfection drug delivery system for antiresorptive therapy. An efficient nonviral gene delivery system is developed using polyethylenimine (PEI) functionalized graphene oxide (GO) complex ...
C. Dou, N. Ding, F. Luo, T. Hou, Z. Cao, Yun Bai, Chuan Liu, Jianzhong Xu, S. Dong   +8 more
semanticscholar   +1 more source

The mechanism of high transfection efficiency of human serum albumin conjugated polyethylenimine in A549 cells

Journal of Medical Sciences, 2015
Background: In our previous study, HSA-PEI demonstrated high pDNA transfection efficiency and low cytotoxicity. Materials and Methods: In this study, the relationship between albumin receptors and the high pDNA transfection efficiency of HSA-PEI was ...
Ssu-Wei Peng, Wei-Hsu Ko, Ming-Kung Yeh, Chiao-Hsi Chiang, Jiin-Long Chen   +4 more
doaj   +1 more source

The thioredoxin‐like and one glutaredoxin domain are required to rescue the iron‐starvation phenotype of HeLa GLRX3 knock out cells

FEBS Letters, EarlyView.
Glutaredoxin (Grx) 3 proteins contain a thioredoxin domain and one to three class II Grx domains. These proteins play a crucial role in iron homeostasis in eukaryotic cells. In human Grx3, at least one of the two Grx domains, together with the thioredoxin domain, is essential for its function in iron metabolism.
Laura Magdalena Jordt, Manuela Gellert, Finja Zelms, Sander Bekeschus, Christopher Horst Lillig   +4 more
wiley   +1 more source

Single Cell Transfection through Precise Microinjection with Quantitatively Controlled Injection Volumes

Scientific Reports, 2016
Cell transfection is a technique wherein foreign genetic molecules are delivered into cells. To elucidate distinct responses during cell genetic modification, methods to achieve transfection at the single-cell level are of great value.
Y. Chow, Shuxun Chen, Ran Wang, Chichi Liu, C. Kong, Ronald A. Li, S. Cheng, Dong Sun   +7 more
semanticscholar   +1 more source

Enhancing HIV-1 Nef Penetration into Mammalian Cells as an Antigen Candidate

Journal of Medical Microbiology and Infectious Diseases, 2019
Introduction: The human immunodeficiency virus type 1 (HIV-1) Nef regulatory protein is known as a candidate for the design of therapeutic HIV DNA and protein vaccines.
Saba Davoodi, Azam Bolhassani, Seyed Mehdi Sadat, Shiva Irani   +3 more
doaj  

Lipsome-mediated uptake of exogenous DNA by Sahiwal cattle spermatozoa

Veterinary World, 2012
Aim: To investigate the influence of lipofection treatment and exogenous DNA uptake on the quality of sahiwal cattle spermatozoa. Materials and Methods: Semen collected from sahiwal bulls (n=7) were evaluated separately for color, volume, mass activity ...
Thomas V. Babu, M. D. Pratheesh, N. Anandlaxmi, K. Amarnath   +3 more
doaj   +1 more source

In vivo evidence for glycyl radical insertion into a catalytically inactive variant of pyruvate formate‐lyase

FEBS Letters, EarlyView.
Dimeric pyruvate formate‐lyase cleaves pyruvate using a radical‐based mechanism. G734 serves as a radical storage location, and the radical is transferred to the catalytic C419 residue. Mutation of the C418‐C419 pair causes loss of enzyme activity, but does not impede radical introduction onto G734. Therefore, cis‐ but not trans‐radical transfer occurs
Michelle Kammel, A. F. Volker Wagner, R. Gary Sawers   +2 more
wiley   +1 more source

Cellular and clinical impact of Haploinsufficiency for genes involved in ATR signaling [PDF]

, 2007
Ataxia telangiectasia and Rad3-related (ATR) protein, a kinase that regulates a DNA damage-response pathway, is mutated in ATR-Seckel syndrome (ATR-SS), a disorder characterized by severe microcephaly and growth delay.
Abraham, Alderton, Alderton, Amenta, Bakhshi, Cardoso, Cardoso, Carr, Chen, Cortez, Cox, Crisponi, De Baere, de Ru, Dierov, Ellison, Fang, Fernandez-Capetillo, Girard, Johanna M. van Hagen, Johnson, Kim, Leventer, Lewis, Liu, Liu, Lupski, Mark O’Driscoll, Noskov, O’Driscoll, O’Driscoll, O’Driscoll, Page, Penny A. Jeggo, Petermann, Redon, Rogers, Seckel, Semmekrot, Shaw-Smith, Sheen, Shiloh, Shizuyo, Stiff, Tassabehji, Tassabehji, Thornburg, Toyo-oka, Wang, William B. Dobyns, Wu, Yao, Yingling, Zachos, Zou, Zou, Zou   +56 more
core   +3 more sources

MET variants with activating N‐lobe mutations identified in hereditary papillary renal cell carcinomas still require ligand stimulation

Molecular Oncology, EarlyView.
MET variants in the N‐lobe of the kinase domain, found in hereditary papillary renal cell carcinoma, require ligand stimulation to promote cell transformation, in contrast to other RTK variants. This suggests that HGF expression in the microenvironment is important for tumor growth in such patients. Their sensitivity to MET inhibitors opens the way for
Célia Guérin, Audrey Vinchent, Marie Fernandes, Isabelle Damour, Agathe Laratte, Rémi Tellier, Gabriella O. Estevam, Jean‐Pascal Meneboo, Céline Villenet, Clotilde Descarpentries, James S. Fraser, Martin Figeac, Alexis B. Cortot, Etienne Rouleau, David Tulasne   +14 more
wiley   +1 more source