Results 161 to 170 of about 928,471 (414)

A helium burst biolistic device adapted to penetrate fragile insect tissues

Journal of Insect Science, 2001
To compensate for the extremely low penetration efficiency of the original PDS/1000-He Bio Rad biolisticreg; device and the deleterious blast effect, design modifications have been made to the launching module.
Jean-Luc Thomas, Jérôme Bardou, Sebastien L'hoste, Bernard Mauchamp, Gérard Chavancy   +4 more
doaj  

Mammalian cell transfection: the present and the future

Analytical and Bioanalytical Chemistry, 2010
Transfection is a powerful analytical tool enabling study of the function of genes and gene products in cells. The transfection methods are broadly classified into three groups; biological, chemical, and physical.
T. Kim, J. Eberwine
semanticscholar   +1 more source

Competence for Transfection in Staphylococcus aureus [PDF]

, 1973
Jan-Eric Sjöström, Martin Lindberg, Lennart Philipson   +2 more
openalex   +1 more source

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio, EarlyView.
ICP34.5 is one of the most important antihost response proteins. The saRNA‐encoding HSV‐1 neurovirulence protein ICP34.5 clearly mediated the eukaryotic initiation factor 2 alpha subunit (eIF2α) dephosphorylation and significant suppression of innate immune responses in vitro, leading to enhanced expression of the saRNA‐encoded gene.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
wiley   +1 more source

Continuous Dynamic Photostimulation - inducing in-vivo-like fluctuating conductances with Channelrhodopsins [PDF]

arXiv, 2013
Central neurons operate in a regime of constantly fluctuating conductances, induced by thousands of presynaptic cells. Channelrhodopsins have been almost exclusively used to imprint a fixed spike pattern by sequences of brief depolarizations. Here we introduce continuous dynamic photostimulation (CoDyPs), a novel approach to mimic in-vivo like input ...
arxiv  

Transfection of Escherichia coli and Salmonella typhimurium Spheroplasts: Host-Controlled Restriction of Infective Bacteriophage P22 Deoxyribonucleic Acid [PDF]

, 1971
Rolf Benzinger, Ingrid Kleber
openalex   +1 more source

Iron‐dependent lysosomal LDL oxidation induces the expression of scavenger receptor A in human THP‐1 monocytes

FEBS Open Bio, EarlyView.
In human monocytic cells THP‐1, a limited uptake of native—not oxidized—LDL/VLDL induced expression of scavenger receptor A and cellular adhesion. Induction was inhibited by lysosomotropic (WR‐1065) and lipophilic (BHT) antioxidants and by siRNAs against ferritinophagy.
Martina Čierna, Richard Buchal, Martin Leníček, Amit Shachak, Jan Pláteník   +4 more
wiley   +1 more source

Synthesis and Characterization of Cationic Glycidyl-Based Poly(aminoester)-Folic Acid Targeting Conjugates and Study on Gene Delivery

Molecules, 2012
A new poly(aminoester) (EPAE-FA) containing folic acid and amino groups in the backbone and side chain was synthesized. EPAE-FA self-assembled readily with the plasmid DNA (pCMV-βgal) in HEPES buffer and was characterized by dynamic light scattering ...
Yu Che Hsiao, Kuo Jung Li, Pai Feng Tsai, Wei Yang Chang, Min Da Shau   +4 more
doaj   +1 more source

Ligand-induced oligomerization of the human GPCR neurotensin receptor 1 monitored in living HEK293T cells [PDF]

arXiv, 2019
The human neurotensin receptor 1 (NTSR1) is a G protein-coupled receptor that can be expressed in HEK293T cells by stable transfection. Its ligand is a 13-amino-acid peptide that binds with nanomolar affinity from the extracellular side to NTSR1. Ligand binding induces conformational changes that trigger the intracellular signaling processes.
arxiv  

Source of the Nonlinear Dependence of Bacteriophage SP82 Transfection on Deoxyribonucleic Acid Concentration [PDF]

, 1971
Herman T. Epstein
openalex   +1 more source