Results 81 to 90 of about 585,058 (294)

T14diLys/DOPE Liposomes: An Innovative Option for siRNA-Based Gene Knockdown?

Pharmaceutics
Background/Objectives: Bringing small interfering RNA (siRNA) into the cell cytosol to achieve specific gene silencing is an attractive but also very challenging option for improved therapies.
Sophie Meinhard, Frank Erdmann, Henrike Lucas, Maria Krabbes, Stephanie Krüger, Christian Wölk, Karsten Mäder   +6 more
doaj   +1 more source

Gene transfection with human hepatocyte growth factor complementary DNA plasmids attenuates cardiac remodeling after acute myocardial infarction in goat hearts implanted with ventricular assist devices [PDF]

, 2005
Yukitoshi Shirakawa, Yoshiki Sawa, Yoshiaki Takewa, Eisuke Tatsumi, Yasufumi Kaneda, Yoshiyuki Taenaka, Hikaru Matsuda   +6 more
openalex   +1 more source

The photoswitchable cannabinoid azo‐HU308 enables optical control of Ca2+ dynamics in INS‐1 β‐cells via off‐target effects on TRPC channels

FEBS Open Bio, EarlyView.
Light activation of the photoswitchable cannabinoid ligand azo‐HU308 triggers Ca2+ influx in pancreatic β‐cells through TRPC channels, independent of CB2 cannabinoid receptors. This reveals a non‐GPCR pathway for cannabinoid modulation of β‐cell Ca2+ dynamics and establishes azo‐HU308 as an optical tool to study cannabinoid signaling through TRP ...
Alexander E. G. Viray, James A. Frank
wiley   +1 more source

Naked cuticle is essential for Drosophila wing development beyond Wingless signaling

FEBS Open Bio, EarlyView.
Naked cuticle (Nkd), a Wnt signaling inhibitor, assumes extensive roles in Drosophila wing development. Overexpressing Nkd causes smaller, crumpled wings, while also perturbing multiple signaling pathways and developmental genes. A specific region (R1S) is critical for Nkd's function as a signaling integrator, offering new insights for studying its ...
Rui Wang, Ping Wang
wiley   +1 more source

Corrigendum: Establishment of an efficient protoplast regeneration and transfection protocol for field cress (Lepidium campestre)

Frontiers in Genome Editing, 2023
Sjur Sandgrind, Xueyuan Li, Emelie Ivarson, Annelie Ahlman, Li-Hua Zhu   +4 more
doaj   +1 more source

Association between adeno‐associated virus genomic titers and intracellular plasmid levels

Engineering Reports
The recombinant adeno‐associated viral (rAAV) vector is one of the most effective viral vectors in gene therapy because of its low immunogenicity, high transduction efficiency, broad tissue specificity, and long‐term transgene expression ability. HEK293T
Kyoko Masumi‐Koizumi, Yuzhe Yuan, Kiyoko Higashiyama, Keisuke Yusa, Kazuhisa Uchida   +4 more
doaj   +1 more source

Transient high-level expression of ß-galactosidase after transfection of fibroblasts from GM1 gangliosidosis patients with plasmid DNA [PDF]

, 2008
Raquel Balestrin, Guilherme Baldo, Matheus Barbosa Vieira, Renata Sano, Janice Carneiro Coelho, Roberto Giugliani, Úrsula da Silveira Matte   +6 more
openalex   +1 more source

Autophagosome marker, LC3, is released extracellularly via several distinct pathways

FEBS Open Bio, EarlyView.
This study establishes a novel HiBiT‐tagging system for ultrasensitive detection of LC3, revealing multiple pathways for its extracellular secretion. It demonstrates that LC3 is released via both autophagy‐dependent and ‐independent mechanisms, including a novel route for nonlipidated LC3‐I.
Koki Saito, Masashi Arakawa, Koki Maeda, Eiji Morita   +3 more
wiley   +1 more source

Effects of PSMA1 on the differentiation and lipid deposition of bovine preadipocytes

Revista Brasileira de Zootecnia
In this study, our goal was to clarify the role of proteasomal subunit α-1 (PSMA1) in both the differentiation of preadipocytes and the accumulation of lipids in adipocytes.
Sheng Li, Chengzhen Chen, Hao Jiang, Jiabao Zhang, Qian Zhou, Yan Gao, Bao Yuan, Mingjun Zhang   +7 more
doaj   +1 more source

DDX3X induces mesenchymal transition of endothelial cells by disrupting BMPR2 signaling

FEBS Open Bio, EarlyView.
Elevated DDX3X expression led to downregulation of BMPR2, a key regulator of endothelial homeostasis and function. Our co‐immunoprecipitation assays further demonstrated a molecular interaction between DDX3X and BMPR2. Notably, DDX3X promoted lysosomal degradation of BMPR2, thereby impairing its downstream signaling and facilitating endothelial‐to ...
Yu Zhang, Jing Wang, De‐Hui Qian, Yang‐Fan Lv, Tian‐Le Cheng, Da‐Peng Wang, Jing Zhang, Ye Fan   +7 more
wiley   +1 more source