Results 171 to 180 of about 3,413,173 (288)

Directed evolution of enzymes at the crossroads of tradition and innovation

FEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova, Andrej Mirossay, Erik Sedlak   +2 more
wiley   +1 more source

Finite-Length Spatiotemporal Modelling for Housing Price Network Spillovers. [PDF]

Entropy (Basel)
Qiu L, Jiao Y, Dong G, Cui G.
europepmc   +1 more source

Small RNA pathways in mammalian oocytes

FEBS Open Bio, EarlyView.
Three distinct small RNA pathways operate in mammalian oocytes: RNAi interference (RNAi), the microRNA (miRNA) pathway, and the PIWI‐associated RNA (piRNA) pathway. These pathways use small RNAs to guide sequence‐specific repression and contribute to oocyte biology by targeting genes and mobile elements or appear insignificant since different ...
Petr Svoboda, Josef Pasulka
wiley   +1 more source

Profitability of Market-based Transfer Price and Cost-based Transfer Price with Cost-reducing Investment [PDF]

In this study, we analyze whether the cost-based transfer price or the market-based transfer price is profitable in divisinalized firms when the upstream division invests to reduce the marginal cost of the products it supplies, without assuming ...
濵村, 純平, ハマムラ, ジュンペイ, 12432, HAMAMURA, Jumpei, 12431   +4 more
core  

Protocol for pH correction of FRET-based fluorescent biosensor imaging in dissociated Drosophila neurons. [PDF]

STAR Protoc
Price MS, Rastegari E, Venkatachalam K.
europepmc   +1 more source

Transcripts enriched in codons that trigger P‐site tRNA‐mediated mRNA decay possess stable mRNA

FEBS Open Bio, EarlyView.
PTMD codons were first described by Mendel et al. as mediators of an mRNA decay pathway dependent on the human protein CNOT3, homologous to yeast Not5. Our findings confirm that PTMD codons destabilize transcripts; however, unlike in yeast, the human pathway specifically targets and slightly destabilizes primarily stable mRNAs.
Rodolfo Lopes Carneiro, Fernando Lucas Palhano   +1 more
wiley   +1 more source

Assessment of noninvasive parameters prior to diagnosis of pulmonary hypertension in patients with fibrotic interstitial lung disease. [PDF]

ERJ Open Res
Herbert S, Dimopoulos K, Jenkin I, Dawes TJW, Bax S, McCabe C, Nashat H, Mukherjee B, Rawal B, Wells AU, Renzoni E, George PM, Jenkins G, Wort SJ, Price LC.   +14 more
europepmc   +1 more source

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

FEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya, Masatake Osawa, Hidefumi Fukumitsu   +2 more
wiley   +1 more source

Enhancing traceability and trust in the sugarcane supply chain using a blockchain-based framework with smart contracts and tokenization. [PDF]

PLoS One
Farooq MS, Qureshi JN, Waheed HA, Khan MA, Farooq U, Shahaab A.   +5 more
europepmc   +1 more source

From energy provision to protein synthesis: Tunnelling nanotubes as mediators of intercellular metabolic cooperation in cancer

FEBS Open Bio, EarlyView.
The cytoskeleton‐mediated transport of mitochondria via tunnelling nanotubes restores respiration, increases ATP production, rescues cells from apoptosis, activates the AKT/mTOR signalling pathway, promotes cell migration and invasiveness, contributes to cancer progression and treatment resistance.
Stanislava Martínková, Jan Trnka
wiley   +1 more source