Formation and removal of 1,N6-dimethyladenosine in mammalian transfer RNA. [PDF]
Nucleic Acids Res, 2022 You XJ +8 more
europepmc +1 more source
Cloning, expression, purification, and structural modeling of the Chandipura virus matrix protein
FEBS Open Bio, EarlyView.This research protocol offers a guide for the cloning, expression, and purification of the Chandipura virus matrix protein using E. coli. It also includes a step‐by‐step procedure for cloning, expressing, and conducting fluorescence imaging of GFP‐fused Chandipura virus matrix protein in mammalian cell lines.
Mariana Grieben
wiley +1 more source
Prediction of the Association between Transfer RNA and Diseases: A Deep Learning Approach Combining Multi-View Graph Convolution and Attention Mechanisms. [PDF]
ACS OmegaLi X, Yao D, Zhang B, Zhan X, Zhang J.
europepmc +1 more source
Amino acids are not all initially attached to the same position on transfer RNA molecules.
, 1975 Thomas H. Fraser, Alexander Rich
openalex +1 more source
Biosynthetic pathway of ribothymidine in B. subtilis and M. lysodeikticus involving different coenzymes for transfer RNA and ribosomal RNA [PDF]
, 1975 Wolfgang E. Schmidt +2 more
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Homologous expression and purification of human HAX‐1 for structural studies
FEBS Open Bio, EarlyView.This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley +1 more source
Unveiling the hidden world of transfer RNA-derived small RNAs in inflammation. [PDF]
J Inflamm (Lond)Qiu P, Jiang Q, Song H.
europepmc +1 more source
Selective Associations of Hormonal Steroids with Aminoacyl Transfer RNAs and Control of Protein Synthesis [PDF]
, 1971 Ruei-Chen Chin, Chev Kidson
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FEBS Open Bio, EarlyView.The relationship between anabolic and catabolic processes governing lung cancer cell growth is nuanced. We show that ATG4B, an autophagy regulator, is elevated in lung cancer and that high ATG4B is associated with worse patient outcomes. Targeting ATG4B in cells reduces growth, protein synthesis, and mTORC1 activity, demonstrating a new relationship ...
Patrick J. Ryan +6 more
wiley +1 more source