Results 51 to 60 of about 404,236 (314)

YAMAT-seq: an efficient method for high-throughput sequencing of mature transfer RNAs. [PDF]

open access: yes, 2017
Besides translation, transfer RNAs (tRNAs) play many non-canonical roles in various biological pathways and exhibit highly variable expression profiles.
Honda, Shozo   +5 more
core   +2 more sources

Efficient cloning system for construction of gene silencing vectors in Aspergillus niger [PDF]

open access: yes, 2008
An approach based on Gateway recombination technology to efficiently construct silencing vectors was developed for use in the biotechnologically important fungus Aspergillus niger. The transcription activator of xylanolytic and cellulolytic genes XlnR of
Graaff, L.H., de   +3 more
core   +2 more sources

Mapping Current Studies of tRNA Fragments onto Disease Landscape

open access: yesBiomolecules
Transfer-RNA-derived fragments (tRFs) are a relatively recently discovered class of non-coding RNAs derived from both precursor and mature transfer RNAs (tRNAs).
Sathyanarayanan Vaidhyanathan   +16 more
doaj   +1 more source

Small RNA populations revealed by blocking rRNA fragments in Drosophila melanogaster reproductive tissues. [PDF]

open access: yesPLoS ONE, 2018
RNA interference (RNAi) is a complex and highly conserved regulatory mechanism mediated via small RNAs (sRNAs). Recent technical advances in high throughput sequencing have enabled an increasingly detailed analysis of sRNA abundances and profiles in ...
Emily K Fowler   +4 more
doaj   +1 more source

Tomato: a crop species amenable to improvement by cellular and molecular methods [PDF]

open access: yes, 1989
Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics. Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures.
A. Crossway   +192 more
core   +8 more sources

Detection of microRNAs in color space [PDF]

open access: yes, 2011
MotivationDeep sequencing provides inexpensive opportunities to characterize the transcriptional diversity of known genomes. The AB SOLiD technology generates millions of short sequencing reads in color-space; that is, the raw data is a sequence of ...
Altschul   +29 more
core   +2 more sources

Transfer RNA halves are found as nicked tRNAs in cells: evidence that nicked tRNAs regulate expression of an RNA repair operon

open access: yesRNA: A publication of the RNA Society, 2023
Transfer RNA fragments are proposed to regulate numerous processes in eukaryotes, including translation inhibition, epigenetic inheritance, and cancer.
Xinguo Chen, S. Wolin
semanticscholar   +1 more source

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

open access: yesFEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman   +3 more
wiley   +1 more source

Ultra-fast conductive media for RNA electrophoretic mobility shift assays

open access: yesBioTechniques, 2020
The use of RNA electrophoretic mobility shift assays (REMSAs) for analysis of RNA–protein interactions have been limited to lengthy assay time and qualitative assessment.
Samantha Z Brown   +3 more
doaj   +1 more source

Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral Preparations

open access: yesFrontiers in Cell and Developmental Biology, 2022
Viruses package host RNAs in their virions which are associated with a range of functions in the viral life cycle. Previous transcriptomic profiling of host RNA packaging mostly focused on retroviruses.
Noah Peña   +21 more
doaj   +1 more source

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