Results 51 to 60 of about 155,620 (276)

Ultra-fast conductive media for RNA electrophoretic mobility shift assays

BioTechniques, 2020
The use of RNA electrophoretic mobility shift assays (REMSAs) for analysis of RNA–protein interactions have been limited to lengthy assay time and qualitative assessment.
Samantha Z Brown, Lebaron C Agostini, Henry L Thomsett, Jonathan R Brody   +3 more
doaj   +1 more source

Profiling Selective Packaging of Host RNA and Viral RNA Modification in SARS-CoV-2 Viral Preparations

Frontiers in Cell and Developmental Biology, 2022
Viruses package host RNAs in their virions which are associated with a range of functions in the viral life cycle. Previous transcriptomic profiling of host RNA packaging mostly focused on retroviruses.
Noah Peña, Wen Zhang, Christopher Watkins, Mateusz Halucha, Hala Alshammary, Matthew M. Hernandez, Matthew M. Hernandez, Wen-Chun Liu, Wen-Chun Liu, Randy A. Albrecht, Randy A. Albrecht, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Adolfo Garcia-Sastre, Viviana Simon, Viviana Simon, Viviana Simon, Viviana Simon, Christopher Katanski, Tao Pan, Tao Pan   +21 more
doaj   +1 more source

Multiple ETS family transcription factors bind mutant p53 via distinct interaction regions

FEBS Letters, EarlyView.
Mutant p53 gain‐of‐function is thought to be mediated by interaction with other transcription factors. We identify multiple ETS transcription factors that can bind mutant p53 and found that this interaction can be promoted by a PXXPP motif. ETS proteins that strongly bound mutant p53 were upregulated in ovarian cancer compared to ETS proteins that ...
Stephanie A. Metcalf, Nicholas F. Downing, Kaitlyn M. Mills, Samuel C. Metcalfe, Alexander E. Kritzer, Lindsey D. Mayo, Peter C. Hollenhorst   +6 more
wiley   +1 more source

An upstream open reading frame regulates expression of the mitochondrial protein Slm35 and mitophagy flux

FEBS Letters, EarlyView.
This study reveals how the mitochondrial protein Slm35 is regulated in Saccharomyces cerevisiae. The authors identify stress‐responsive DNA elements and two upstream open reading frames (uORFs) in the 5′ untranslated region of SLM35. One uORF restricts translation, and its mutation increases Slm35 protein levels and mitophagy.
Hernán Romo‐Casanueva, Ariann E. Mendoza‐Martínez, P. Abril Medina‐Flores, Carlos Campero‐Basaldua, Alexander DeLuna, Soledad Funes   +5 more
wiley   +1 more source

tiRNAs: Insights into Their Biogenesis, Functions, and Future Applications in Livestock Research

Non-Coding RNA, 2022
Transfer RNA (tRNA)-derived small RNAs (tsRNAs) belong to a group of transfer ribonucleic acid (tRNA)-derived fragments that have recently gained interest as molecules with specific biological functions.
Fabio Sarais, Alvaro Perdomo-Sabogal, Klaus Wimmers, Siriluck Ponsuksili   +3 more
doaj   +1 more source

Sequence determinants of RNA G‐quadruplex unfolding by Arg‐rich regions

FEBS Letters, EarlyView.
We show that Arg‐rich peptides selectively unfold RNA G‐quadruplexes, but not RNA stem‐loops or DNA/RNA duplexes. This length‐dependent activity is inhibited by acidic residues and is conserved among SR and SR‐related proteins (SRSF1, SRSF3, SRSF9, U1‐70K, and U2AF1).
Naiduwadura Ivon Upekala De Silva, Puspa Kunwar, Md Ibnul Rifat Rahman, Joanna Koryo Kwao, Nathan Lehman, Zihan Zhang, Trenton Paul, Claire Cheng, Nicholas Truex, Hui‐Ting Lee, Jun Zhang   +10 more
wiley   +1 more source

Mapping m⁶A Sites on HIV-1 RNA Using Oligonucleotide LC-MS/MS

Methods and Protocols
The biological significance of chemical modifications to the ribonucleic acid (RNA) of human immunodeficiency virus type-1 (HIV-1) has been recognized.
Alice Baek, Asif Rayhan, Ga-Eun Lee, Sarah Golconda, Hannah Yu, Shihyoung Kim, Patrick A. Limbach, Balasubrahmanyam Addepalli, Sanggu Kim   +8 more
doaj   +1 more source

Characterization of the complete mitochondrial genome of band-rumped Storm-petrel, Hydrobates castro

Mitochondrial DNA. Part B. Resources, 2018
In this study, the complete mitochondrial genome of band-rumped Storm-petrel, Hydrobates castro, was determined through sequencing of PCR fragments. The complete mitochondrial genome of H.
Jin-Qing Jiang, Qing-Hua Wang, Tian-Zeng Gao   +2 more
doaj   +1 more source

Ribozyme activity modulates the physical properties of RNA–peptide coacervates

eLife, 2023
Condensed coacervate phases are now understood to be important features of modern cell biology, as well as valuable protocellular models in origin-of-life studies and synthetic biology.
Kristian Kyle Le Vay, Elia Salibi, Basusree Ghosh, TY Dora Tang, Hannes Mutschler   +4 more
doaj   +1 more source

Cell density–dependent nuclear‐cytoplasmic shuttling of SETDB1 integrates with Hippo signaling to regulate YAP1‐mediated transcription

FEBS Letters, EarlyView.
At low cell density, SETDB1 and YAP1 accumulate in the nucleus. As cell density increases, the Hippo pathway is gradually activated, and SETDB1 is associated with increased YAP1 phosphorylation. At high cell density, phosphorylated YAP1 is sequestered in the cytoplasm, while SETDB1 becomes polyubiquitinated and degraded by the ubiquitin–proteasome ...
Jaemin Eom, Jaewoong Jang, Jung Sun Park, Yong‐Kook Kang   +3 more
wiley   +1 more source