Results 101 to 110 of about 3,602,698 (355)

The atypical KRASQ22K mutation directs TGF‐β response towards partial epithelial‐to‐mesenchymal transition in patient‐derived colorectal cancer tumoroids

Molecular Oncology, EarlyView.
TGF‐β has a complex role in cancer, exhibiting both tumor‐suppressive and tumor‐promoting properties. Using a series of differentiated tumoroids, derived from different stages and mutational background of colorectal cancer patients, we replicate this duality of TGF‐β in vitro. Notably, the atypical but highly aggressive KRASQ22K mutation rendered early‐
Theresia Mair, Philip König, Milena Mijović, Jessica Kalla, Anil Baskan, Loan Tran, Kristina Draganić, Pedro Morata Saldaña, Carlos Uziel Pérez Malla, Janette Pfneissl, Andreas Tiefenbacher, Julijan Kabiljo, Velina S. Atanasova, Lisa Wozelka‐Oltjan, Leonhard Müllauer, Michael Bergmann, Raheleh Sheibani‐Tezerji, Gerda Egger   +17 more
wiley   +1 more source

Model for Polymorphic Transitions in Bacterial Flagella [PDF]

Phys. Rev. E 73, 011902 (2006), 2005
Many bacteria use rotating helical flagellar filaments to swim. The filaments undergo polymorphic transformations in which the helical pitch and radius change abruptly. These transformations arise in response to mechanical loading, changes in solution temperature and ionic strength, and point substitutions in the amino acid sequence of the protein ...
arxiv   +1 more source

Genetic Studies of Sulfadiazine-resistant and Methionine-requiring \u3cem\u3eNeisseria\u3c/em\u3e Isolated From Clinical Material [PDF]

, 1967
Deoxyribonucleate (DNA) preparations were extracted from Neisseria meningitidis (four isolates from spinal fluid and blood) and N. gonorrhoeae strains, all of which were resistant to sulfadiazine upon primary isolation.
BROWN G. M., CATLIN B. W., CATLIN B. W., CATLIN B. W., CATLIN B. W., CAUTHEN S. E., DAVIS B. D., DAVIS B. D., DEMAIN A. L., DEMEREC M., E., DONALD I. J., DU VIGNEAUD V., W., EICKHOFF T. C., GRABOW W., HARRIS J. S., HATCH F. T., HOTCHKISS R. D., KAPLAN M. M., KATZEN H. M., KOHN H. I., LANDY M., LEDERBERG J., MILLAR J. W., OAKBERG E. F., PATO M. L., REYN A., REYN A., ROWBURY R. J., SICARD A. M., SINGER S., G., SMITH D. A., WILLCOX R. R., WINKLER K. C., WOLF B., WOODS D. D., WOODS D. D.   +35 more
core   +2 more sources

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

The Landscape of Realized Homologous Recombination in Pathogenic Bacteria [PDF]

, 2015
Recombination enhances the adaptive potential of organisms by allowing genetic variants to be tested on multiple genomic backgrounds. Its distribution in the genome can provide insight into the evolutionary forces that underlie traits, such as the ...
Didelot, X, Falush, D, Jolley, KA, Kobayashi, I, Maiden, MCJ, Sheppard, SK, Yahara, K   +6 more
core   +2 more sources

Factors affecting genetic transformation via Agrobacterium tumefaciens in Phaseolus vulgaris L.

Biotecnología Vegetal, 2013
Phaseolus genus, as well as other legumes, has proved recalcitrant plant regeneration in vitro and mainly to genetic transformation via Agrobacterium tumefaciens.
Amanda Martirena-Ramírez, Novisel Veitía   +1 more
doaj  

Enhanced discovery of bacterial laccase‐like multicopper oxidase through computer simulation and metagenomic analysis of industrial wastewater

FEBS Open Bio, EarlyView.
We obtained potential bacterial laccase‐like multicopper oxidase (LMCO) sequences through metagenomic sequencing. All sequences exhibited significant differences from known LMCOs in databases. To select the most promising candidates, we performed structure prediction and molecular docking using alphafold2, metal3d and rosetta.
Ting Cui, Ariel Kushmaro, Hana Barak, Anja Poehlein, Rolf Daniel, Hans‐Jürgen Mägert   +5 more
wiley   +1 more source

MOLECULAR CLONING OF OVINE cDNA LEPTIN GENE

Scientific Papers Animal Science and Biotechnologies, 2023
An efficient bacterial transformation system suitable for cloning the coding sequence of the ovine leptin gene in E. coli DH5α host cells using the pGEMT easy vector it is described in this paper.
CLAUDIA TEREZIA SOCOL, A. VLAIC, VIORICA COŞIER   +2 more
doaj  

Electrosprayed chitosan nanoparticles: facile and efficient approach for bacterial transformation

International Nano Letters, 2017
A rapid and efficient procedure for DNA transformation is a key prerequisite for successful cloning and genomic studies. While there are efforts to develop a facile method, so far obtained efficiencies for alternative methods have been unsatisfactory (i ...
M. Abyadeh, Esmaeil Sadroddiny, A. Ebrahimi, F. Esmaeili, F. S. Landi, A. Amani   +5 more
semanticscholar   +1 more source

Comparative study of adenosine 3′‐pyrophosphokinase domains of MuF polymorphic toxins

FEBS Open Bio, EarlyView.
With the ultimate goal of understanding the association of toxin‐immunity modules to temperate phages, we characterized toxins from three prophages and examined cross‐protection from immunity proteins. The toxins exhibit adenosine 3′‐pyrophosphokinase activity and are toxic in Escherichia coli.
Eloïse M. Paulet, Julia Bartoli, Atakan Kabtan, Chloé M. Piras, Audrey C. Tempier, Eric Cascales, Julie P. Viala   +6 more
wiley   +1 more source