Results 181 to 190 of about 3,602,698 (355)

Alternative design of a bacterial transformation chip

, 2022
Traditionally, bacterial transformations are carried in small microcentrifuge tubes. But, there is a possibility that spatial confinement in small microchannels can facilitate plasmid entry into competent cells. This gave rise to the relatively new concept of chip-based transformation where competent cells and assembled plasmids are mixed on a ...
openaire   +1 more source

Bacteria‐Derived Extracellular Vesicle as A “Trojan Horse” for Selective M1 Macrophage‐Targeting in A Multi‐Cellular Entanglement Environment

Advanced Functional Materials, EarlyView.
The temporary transition of macrophages from a pro‐inflammatory phenotype of macrophages (M1) to an anti‐inflammatory phenotype of macrophages (M2) is crucial for tissue repair and regeneration processes. Bacterial outer membrane vesicles (OMVs) are utilized as a “trojan horse” for specific M1 macrophage‐targeting and anti‐inflammatory drug delivery ...
Donglin Cai, Zhelun Li, Wendong Gao, Yuqing Mu, Jiaying Liu, Yufeng Zhang, Xiaohan Mei, Jingjing Quan, Lan Xiao, Yin Xiao   +9 more
wiley   +1 more source

Transmembrane Activation of Catalysis and Protein Refolding in Synthetic Cells by Enzymes and Nanozymes

Advanced Functional Materials, EarlyView.
Synthetic cells are engineered herein to respond to an external chemical messenger by the activation of intracellular catalysis. The chemical messenger molecules are catalytically generated by an extracellular enzyme or a mineral surface, whereas the intracellular catalysis emerges via direct enzyme activation or via protein refolding.
Dante G. Andersen, Andreas B. Pedersen, Mireia Casanovas Montasell, Søren Moesgaard, Anja Bille Bohn, Alexander N. Zelikin   +5 more
wiley   +1 more source

Quantitative Aspects, Engineering and Optimization of Bacterial Sensor Systems [PDF]

arXiv
Bacterial sensor systems can be used for the detection and measurement of molecular signal concentrations. The dynamics of the sensor directly depend on the biological properties of the bacterial sensor cells; manipulation of these features in the wet lab enables the engineering and optimization of the bacterial sensor kinetics.
arxiv  

Competence induction of homologous recombination genes protects pneumococcal cells from genotoxic stress

mBio
Homologous recombination (HR) is a universally conserved mechanism of DNA strand exchange between homologous sequences, driven in bacteria by the RecA recombinase.
David De Lemos, Anne-Lise Soulet, Violette Morales, Mathieu Berge, Patrice Polard, Calum Johnston   +5 more
doaj   +1 more source

Temperature‐Enhanced Supramolecular Polymer Adhesion Provided by Concurrent Utilization of Calix[4]Pyrrole and Crown Ether Molecular Recognition

Advanced Functional Materials, EarlyView.
Simultaneous utilization of calix[4]pyrrole‐ and crown ether‐based molecular recognition allows the construction of a high molecular weight alternating supramolecular polymer. This heat processible polymer can be used as a durable and reusable adhesive on glass and steel with temperature‐enhanced adhesion strength without the need for a solvent ...
Deniz Memis, Abdullah Aydogan
wiley   +1 more source

Bacterial swimming and accumulation on endothelial cell surfaces [PDF]

arXiv
Flagellar-driven locomotion plays a critical role in bacterial attachment and colonization of surfaces, contributing to the risks of contamination and infection. Tremendous attempts to uncover the underlying principles governing bacterial motility near surfaces have relied on idealized assumptions of surrounding inorganic boundaries.
arxiv  

Binding and entry of DNA in bacterial transformation

, 1976
I would like to thank Dr A. Rosenthal for critically reading parts of the manuscript and Mr B. Greenberg for assistance with the references. Unpublished work from the author’s laboratory was carried out under the auspices of the U.S. Atomic Energy Commission and the U.S. Energy and Research Administration.
openaire   +3 more sources

Pneumococcal extracellular vesicles mediate horizontal gene transfer via the transformation machinery

mSphere
Bacterial cells secrete extracellular vesicles (EVs), the function of which is a matter of intense investigation. Here, we show that the EVs secreted by the human pathogen Streptococcus pneumoniae (pneumococcus) are associated with bacterial DNA on their
Sarah Werner Lass, Bailey E. Smith, Shaw Camphire, Rory A. Eutsey, Jojo A. Prentice, Saigopalakrishna S. Yerneni, Ashni Arun, Andrew A. Bridges, Jason W. Rosch, James F. Conway, Phil Campbell, N. Luisa Hiller   +11 more
doaj   +1 more source

Ultrasoft Iontronics: Stretchable Diodes Enabled by Ionically Conductive Bottlebrush Elastomers

Advanced Functional Materials, EarlyView.
This work introduces a solvent‐free, ultrasoft, and stretchable ionic diode based on oppositely charged bottlebrush elastomers (BBEs). The BBE diode exhibits an ultralow Young's modulus (<23 kPa), a high rectification ratio of 46, and stretchability over 400%.
Xia Wu, Pengfei Xu, Zefang Zhang, Qi Yang, Xi Huang, Peng Pan, Xinyu Liu   +6 more
wiley   +1 more source